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About

The Biomics Pole is the CITECH structure at Institut Pasteur for Next Generation Sequencing. Biomics is headed by Sean Kennedy. Biomics handles both service and collaborative projects and works closely with the C3BI and DSI to provide data analysis solutions and data storage for researchers. Please contact us for pricing and turnaround times, or for any questions you might have.

Download Project Submission Form

Ask a short or technical question about your project, our services or technology

The Biomics Pole’s mission is to collaborate with the Pasteur Institute’s international network, with diverse research teams and with private industry in order to provide a service to the scientific community. The complementary technologies and synergistic expertise of the different teams at the pole facilitates the investigation of complex biological problems from DNA sequencing to gene expression and epigenetics and through to data analysis.

  • Some examples of what we provide are:
    – De novo and targeted sequencing of viruses, prokaryotes and eukaryotes
    – Variant (SNP, indel, large rearrangements) detection
    – Human and Mouse SNP detection by array
    – Transcriptional analysis (RNA-Seq) for both prokaryotes and eukaryotes
    – 16S and deep-sequencing metagenomic studies (mouse, human, and other environments)
    – Epigenetics (methylation studies)
    – Projects involving two or more techniques (i.e. proteogenomics, single-cell DNA/RNA analysis)

In addition to projects that can be realized directly at the Biomics Pole, we can also provide contacts and expertise for other technologies including Whole Human Genome Sequencing (Illumina X10 or X5), Ion Proton/PGM and PacBio.

Biomics and it staff of experts works hard for the successful and timely processing of all samples and requests. If you are unsatisfied with our service or have a suggestion on how we can improve please do not hesitate to contact us: Reclamation / Suggestions

News

Members

Projects

Transversal Project

Software

Tools

Services

Training on the Agilent Bioanalyzer

A SPOC for using the Agilent Bioanalyzer Training sessions for the use of the Bioanalyzer, organized at the Transcriptome and Epigenome platform of the Biomics pole, are now replaced by a SPOC, available 24 […]

Pushing the Limit: A Complete Solution for Generating Stranded RNA-Seq Libraries from Picogram Inputs of Total Mammalian RNA

With challenging samples, like RNA isolated using laser capture microscopy, it is difficult to obtain 10 ng of starting material; additionally, rRNA depletion leads to sample loss, undermining robust library preparation. The new release surpasses […]

Index compatibility for sequencing

Illumina uses a green laser to sequence G/T and a red laser to sequence A/C. At each cycle at least one of two nucleotides for each color channel need to be read to ensure […]

Protocol for library construction available allowing to perform RNAseq from low input

The SMARTer Ultra Low Input RNA Kit for Sequencing – v3 incorporates Clontech’s patented SMART (Switching Mechanism at 5’ End of RNA Template) technology. This technology uses the template switching activity of reverse transcriptase […]

Single Cell

RNAseq is routinely used for the characterization of bulk transcriptome. Nevertheless, Interest in single-cell whole-transcriptome analysis is growing rapidly, especially for profiling rare or heterogeneous populations of cells. In collaboration with the Centre d’Immunologie […]

CHIPSeq

ChIPSeq is used for the analysis of the interactions between the genome and nucleic acids binding proteins like transcription factors or chromatin associated proteins. Starting from the IPs / inputs, the PF can perform […]

RNAseq

RNAseq is used for gene expression profiling of different populations of coding or non coding RNAs as well as for the annotation of the genome (exhaustive mapping of TSS, identification of splicing isoforms…). Several […]

Equipments

Sequencing Systems • Illumina • MiSeQ

Here is the list of type runs on MiSeq :List_runs_MiSeq  

BioAnalyzer • Agilent • Bioanalyzer 2100

Our Bioanalyzer 2100 is dedicated to the microfluidic  electrophoresis of nucleic acids (DNA, RNA). Depositing the samples is done on specific chips depending on the type of sample. Electrophoresis is fast and provided quantitative […]

Sequencing Systems • Illumina • HiSeq 2000

Illumina sequencing devices which are shared with other platforms of the Biomics Pole

Sequencing Systems • Illumina • Hiseq 2500 (1To)

Illumina sequencing devices which are shared with other platforms of the Biomics Pole

Fundings

Publications