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About

The Biomics Pole is the C2RT structure at Institut Pasteur for Next Generation Sequencing and includes both short and long-read technologies.

Our mission is to facilitate scientific discovery within Pasteur Institute’s network, with diverse research teams and with private industry. Biomics accepts both service and collaborative projects and works closely with the C3BI and DSI to provide data analysis solutions and data storage for researchers. Please contact us for pricing and turnaround times.  Submit a Project 

  • Some examples of what we provide are:
    – De novo and targeted sequencing of viruses, prokaryotes and eukaryotes
    – Variant (SNP, indel, large rearrangements) detection
    – Human and Mouse SNP detection by array
    – Transcriptional analysis (RNA-Seq) for both prokaryotes and eukaryotes
    – 16S and deep-sequencing metagenomic studies (mouse, human, and other environments)
    – Epigenetics (methylation studies)
    – Long-Read PacBio analysis for DNA-Seq and Transcriptomics (Iso-Seq)

In addition to projects that can be realized directly at the Biomics Pole, we can also provide contacts and expertise for other technologies including Whole Human Genome Sequencing (Illumina / Chromium), or Ion Proton/PGM.

Ask a question about techniques, our services or sequencing technology

Biomics and it staff of experts works hard for the successful and timely processing of all samples and requests. If you are unsatisfied with our service or have a suggestion on how we can improve please do not hesitate to contact us: Reclamation / Suggestions

Research group wishing to include significant NGS sequencing/analysis in grant applications, or who require aid in writing are encouraged to contact us prior to submission: Include Biomics in request for funding

Members

Projects

Transversal Project

Software

Tools

Services

Training on the Agilent Bioanalyzer

A SPOC for using the Agilent Bioanalyzer Training sessions for the use of the Bioanalyzer, organized at the Transcriptome and Epigenome platform of the Biomics pole, are now replaced by a SPOC, available 24 […]

Pushing the Limit: A Complete Solution for Generating Stranded RNA-Seq Libraries from Picogram Inputs of Total Mammalian RNA

With challenging samples, like RNA isolated using laser capture microscopy, it is difficult to obtain 10 ng of starting material; additionally, rRNA depletion leads to sample loss, undermining robust library preparation. The new release surpasses […]

Index compatibility for sequencing

Illumina uses a green laser to sequence G/T and a red laser to sequence A/C. At each cycle at least one of two nucleotides for each color channel need to be read to ensure […]

Protocol for library construction available allowing to perform RNAseq from low input

The SMARTer Ultra Low Input RNA Kit for Sequencing – v3 incorporates Clontech’s patented SMART (Switching Mechanism at 5’ End of RNA Template) technology. This technology uses the template switching activity of reverse transcriptase […]

Single Cell

RNAseq is routinely used for the characterization of bulk transcriptome. Nevertheless, Interest in single-cell whole-transcriptome analysis is growing rapidly, especially for profiling rare or heterogeneous populations of cells. In collaboration with the Centre d’Immunologie […]

CHIPSeq

ChIPSeq is used for the analysis of the interactions between the genome and nucleic acids binding proteins like transcription factors or chromatin associated proteins. Starting from the IPs / inputs, the PF can perform […]

RNAseq

RNAseq is used for gene expression profiling of different populations of coding or non coding RNAs as well as for the annotation of the genome (exhaustive mapping of TSS, identification of splicing isoforms…). Several […]

Equipments

Sequencing Systems • Illumina • MiSeQ

Here is the list of type runs on MiSeq :List_runs_MiSeq  

BioAnalyzer • Agilent • Bioanalyzer 2100

Our Bioanalyzer 2100 is dedicated to the microfluidic  electrophoresis of nucleic acids (DNA, RNA). Depositing the samples is done on specific chips depending on the type of sample. Electrophoresis is fast and provided quantitative […]

Sequencing Systems • Illumina • HiSeq 2000

Illumina sequencing devices which are shared with other platforms of the Biomics Pole

Sequencing Systems • Illumina • Hiseq 2500 (1To)

Illumina sequencing devices which are shared with other platforms of the Biomics Pole

Fundings

Publications

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