Clostridium difficile a Gram-positive, anaerobic, spore forming bacterium, is the major causative agent of the antibiotic-associated diarrhoea and is responsible of the majority of the pseudomembranous colitis. It is becoming the main enteropathogen bacterium isolated in nosocomial diarrhoea of adults. C. difficile is acquired through ingestion of spores shed into the environment. Production of two toxins plays a major role in its pathogenicity but many virulence factors seem to be involved during the gut colonization and the process of C. difficile infection (CDI). The main goals of our research are
- To better understand the mechanisms by which the synthesis of C. difficile toxins is regulated and secreted, especially in relation to cellular metabolic pathways;
- To identify the bacterial features that must be considered in the pathogenic process of C. difficile including colonization factors, biofilm and sporulation;
- And to identify still unknown epidemic and emergence characters of recent hypervirulent strains.