The Center for Technological Resources and Research (C2RT) is one of the four components of the Technology Department. C2RT gathers the Technology and Service units (UTechS) and the technological core facilities of the campus, which are described below. C2RT also has a technology laboratory (TechLab) located at the first floor of the François Jacob building. The TechLab allows to host projects of technological research and to test new service activities. The TechLab works on a project basis and is accessible to the Institut Pasteur teams, possibly in collaboration with external academic or industrial teams. The Center for Technological Resources and Research is under the scientific responsibility of Spencer Shorte.
Contact: c2rt@pasteur.fr
Mission
The mission of the C2RT is to support the Institut Pasteur research teams and scientific departments in achieving their research objectives by addressing their present and future technology needs.
C2RT aims at:
- → Providing researchers with state-of-the-art equipment, services and technical expertise to enable the success of their projects
- → Providing training and education on advanced technologies and methods
- Sourcing new and emerging technologies through the technological watch
- Proposing technological and methodological developments to the research teams
- Identifying in close connection with the Scientific Deparments their strategic needs for equipment and expertise
Organized in Technology Service Units and Technological Core Facilities covering a wide range of technological areas, C2RT is a key component of Institut Pasteur’s research ecosystem.
Certified training courses
Working with C2RT
The C2RT entities operate on a project basis. To submit a project application or ask your questions, you can directly contact the entity of your choice for a one-to-one meeting or attend one of the open-desks that are regularly organized.
- C2RT booklet: click here to obtain the booklet
- Where to find the C2RT technology core facilities: Click here to obtain the map
- Guidelines for users and core facility teams: click here to obtain the guide Click here to obtain the guide
You have not found what you were looking for ? Please contact us at: c2rt@pasteur.fr For advice or support in managing your data, please contact the Data Management Core Facility of the Technology Department. Click here for more information.
C2RT Technology Units and Technological Core Facilities
Head: Spencer Shorte, Interim manager and head of the photonic bio-imaging technology platform: Nathalie Aulner UTechS PBI provides expertise and support in optical imaging by supplying the users with a diverse range of equipment (confocal microscopy, super-resolution microscopy, high-throughput and high-content imaging, small animal imaging…). The development activities focus on the study of host-pathogen interactions and high-content imaging.
Ultrastructural Bioimaging Core Facility
Head: Adeline Mallet, UBI provides expertise and support in electronic imaging, with a set of sample preparation techniques and sample visualization in transmission or scanning electron microscopy. The expertise of the UTechS particularly lies in the imaging of pathogens and host-pathogen interactions.
Head: Jean-Yves Tinevez The Image analysis Hub provides an expertise in the use of commercial or Institut Pasteur-developed softwares for the analysis of images produced by using the equipment available in the UTechS PBI and UBI as well as in the research units.
UTechS Mass Spectrometry for Biology
Head: Julia Chamot-Rooke, Head of the Proteomic core facility: Mariette Matondo UTechS MSBio offers research and service activities in the field of biological mass spectrometry and proteomics. The service activities cover a range of approaches ranging from the identification of isolated proteins to the analysis of proteomes.
UTechS Cytometry and Biomarkers
Head: Milena Hasan, Head of the Cytometry core facility: Sophie Novault UTechS Cytometry and Biomarkers has a wide range of equipment to achieve cellular sorting, cell phenotyping, and single cell profiling and sequencing. UTechS cytometry and biomarkers is also attached to the Center for Translational Research
Head: Marc Monot The Biomics core facility conducts nucleic acid sequencing projects (genomes, transcriptomes, epigenomes, metagenomes and metatranscriptomes) using next-generation sequencers (Illumina, PacBio, Miniion). It also has a specific equipment for the genotyping of eukaryotic genomes (man, mouse).
Antibody Engineering Core Facility
Head : Pierre Lafaye The Antibody Engineering core facility is specialized in the identification and production of homodimeric antibodies of camelids (VHH).
Production and Purification of Recombinant Proteins Core Facility
Head: Stéphane Pêtres The Production and Purification of Recombinant Proteins core facility offers services in the field of the production and purification of recombinant proteins in eukaryotic systems (mammal or insect) or prokaryotes (bacteria or yeast).
Molecular Biophysics Core Facility
Head: Patrick England. The Molecular Biophysics core facility offers a set of equipment and technical and methodological expertise for the molecular-scale characterization of biological macromolecules.
Head: Ahmed Haouz The crystallography core facility offers the expertise and the technological environment enabling robotic crystal growth, X-ray diffraction and the analysis of the resulting data.
Head: Inaki Guijarro The NMR in biology core facility has several NMR spectrometers for the study of Biological macromolecules, their dynamics and atomic-scale interactions. The core facility also offers an expertise in HDX mass spectrometry.
Chemogenomic and Biological Screening Core Facility
Head: Fabrice Agou The Chemogenomic and Biological Screening core facility supports the scientists from the development of targeted or phenotypic screens, to the automation and miniaturization of these screens and the identification of molecules or genetic modulators of interest.
Biomaterials and Microfluidics Core Facility
Head: Samy Gobaa The Biomaterials and Microfluidics core facility provides the necessary equipment and expertise in the fields of micro-manufacturing, photo-and soft-lithography, multi-parameter cell screening and automated live cell imaging. It also provides expertise in 3D cell culture and organ-on-chip.
Head: Matthijn Vos The NanoImaging Core facility (NCF) provides high throughput cryoelectro microscopy in order to resolve the structure of isolated proteins in solution as well as imaging at the nanometer scale inside the cellular environement.
High Performance Computing Core Facility
Head: Youssef Ghorbal The High performance Computing Core facility (HPC) main mission evolves around providing and maintaining comprehensive HPC resources and services for the Institut Pasteur’s researchers. At first, the core facility handles setup operation of HPC clusters (systems administration, availability and upgrades). It also provides an end user support ranging from cluster access and on-boarding to efficient resources usage recommendations. The HPC core facility manages a large software collections on the HPC clusters and offers profiling and optimization services. The HPC also develops specific software in collaboration with other core facilities or research units.
