The objective of the platform is to bridge biology and engineering in order to help the development of biomedical projects with a strong technological focus. Practically we provide the campus with essential technological bricks and expertise in microfluidics and biomaterials. Since May 2015 we developed in-house capabilities in the field of microfabrication, photo- and soft-lithography, multifactorial cell screening and automated live cell imaging especially targeted for the better understanding of complex cell-microenvironment interactions. We also provide assistance with the deployment of advanced 3D cell culture and organ-on-chip systems.
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Connections
Members
Former Members
Projects
Services
Advanced 3D cell culture
We provide a library of 3D cell culture scaffolds with tunable biophysical and biochemical properties.
Soft-lithography bench
The pipeline is targeted for the production of microfluidics chips in PDMS (silicone elastomer) . Masters produced by contact photolithography are (negatively) replicated in PDMS under dust-free conditions. The bonding of the chips are performed […]
Contact Photolithography
The photolithography station is dedicated to microfabrication applications. The concept is based on the using 1-UV light, 2-Binary masks and 3-photosensitive resins to produce 3D objects with resolution <1μm.
Equipments
2 • Microfluidic syringe pumps • Cetoni • neMESYS
Computer controlled (programmable) syringe pump system dedicated to microfluidic chip perfusion. One controller plus two low pressure modules and a pressure sensor.
Inverted microscope for live cell imaging • Zeiss • Axiovert
Inverted microscope for live cell imaging capabilities. The microscope is built for speed in order to reduce the interval between time points when acquiring a timelapse experiment. Standard LED fluorescence is available (Colibri system). […]
Fundings
Publications
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2017A tuneable microfluidic system for long duration chemotaxis experiments in a 3D collagen matrix, Lab Chip 2017 Nov;17(22):3851-3861.
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2016Controlled production of sub-millimeter liquid core hydrogel capsules for parallelized 3D cell culture, Lab Chip 2016 12;17(1):110-119.
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2015Paramecium swimming and ciliary beating patterns: a study on four RNA interference mutations, Integr Biol (Camb) 2015 Jan;7(1):90-100.
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2013Cellular capsules as a tool for multicellular spheroid production and for investigating the mechanics of tumor progression in vitro, Proc. Natl. Acad. Sci. U.S.A. 2013 Sep;110(37):14843-8.
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2010Synergistic effects of metal nanoparticles and a phenolic uncoupler using microdroplet-based two-dimensional approach, J Environ Monit 2011 Feb;13(2):410-5.
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2007Micro fluid segment technique for screening and development studies on Danio rerio embryos, Lab Chip 2007 Sep;7(9):1132-8.
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Contact
Phone: +33 1 44 38 92 75
Email: bioengineeringservices@pasteur.fr; samy.gobaa@pasteur.fr
Address: 25-28 Rue du Docteur Roux 75015, Paris France