The objective of the platform is to bridge biology and engineering in order to help the development of biomedical projects with a strong technological focus. Practically we provide the campus with essential technological bricks and expertise in microfluidics and biomaterials. Since May 2015 we developed in-house capabilities in the field of microfabrication, photo- and soft-lithography, multifactorial cell screening and automated live cell imaging especially targeted for the better understanding of complex cell-microenvironment interactions. We also provide assistance with the deployment of advanced 3D cell culture and organ-on-chip systems.
Microfluidic Large-Scale Integration Applied to Molecular Diagnostics. Prof. Sebastian MAERKL (EPFL)
Microfluidics Club at Pasteur Third Seminar: Dr. Gilgueng HWANG
The goals of this project are to build an external bioartificial liver, a liver-on-chip as well as a bioengineered liver. In the present project, bioconstruction of the liver will be performed by the assembly […]
Welcome to the club! Welcome to Microfluidics@Pasteur! Our goal is to serve as the major entry point to all the scientists on the campus (beginners and experts) interested in undertaking projects where microfluidics is involved. Our […]
We provide a library of 3D cell culture scaffolds with tunable biophysical and biochemical properties.
The pipeline is targeted for the production of microfluidics chips in PDMS (silicone elastomer) . Masters produced by contact photolithography are (negatively) replicated in PDMS under dust-free conditions. The bonding of the chips are performed […]
The photolithography station is dedicated to microfabrication applications. The concept is based on the using 1-UV light, 2-Binary masks and 3-photosensitive resins to produce 3D objects with resolution <1μm.
Programmable spincoater dedicated to the production of thin layers of PDMS
Computer controlled (programmable) syringe pump system dedicated to microfluidic chip perfusion. One controller plus two low pressure modules and a pressure sensor.
Inverted microscope for live cell imaging capabilities. The microscope is built for speed in order to reduce the interval between time points when acquiring a timelapse experiment. Standard LED fluorescence is available (Colibri system). […]
Oxygen plasma cleaner. Applications: surface activation, PDMS bonding, cleaning. Controllable vacuum, gas injection, power, treatment time.
Manual mask aligner equipped with a topside microscope and dual objectives. Micrometric screws allow a precise mask-substrate alignment.