The “Molecular Biophysics” facility (french acronym: Plate Forme de Biophysique MoleculaIre, PFBMI) federates an ensemble of state-of-the-art instrumentation for the in vitro molecular-scale characterization of biological macromolecules, together with well established technical and methodological expertise
What we do ?
The mission of the PFBMI is to provide an added value to a great variety of biological projects, enabling the characterization of the intrinsic properties of macromolecules and their assemblies (size, shape, folding, stability auto-association,…) and of the interactions in which they are involved (stoichiometry, thermodynamic and kinetic parameters, …).
PFBMI equipments are available for the entire scientific community, in France and abroad, but a priority is given to teams from Institut Pasteur and the Institut Pasteur International Network.
Pricing : services are charged according to the following downloadable chart
Steering committee: Jean-Baptiste CHARBONNIER (CEA Saclay), Alexandre CHENAL (Institut Pasteur), Katherine STOTT (University of Cambridge), Nicolas WOLFF (Institut Pasteur)
The PFBMI follows the best practice guidelines of C2RT
Fields of expertise of the PFBMI :
- Size and shape of macromolecules and their assemblies
- Stoichiometry of macromolecular assemblies
- Secondary and tertiary structures of macromolecules
- Thermodynamic stability of macromolecules
- Characterisation of molecular interactions
- Quality control (QC) of purified proteins
To submit a project to the PFBMI :
Technologies available at the PFBMI :
- Analytical ultracentrifugation (AUC) XL-I and Optima AUC
- Circular dichroism (CD)
- Dynamic light scattering (DLS) cuvette or plate reader
- Fluorescence spectroscopy (steady state or stopped flow)
- Isothermal Microcalorimetry (ITC)
- Differential scanning calorimetry (DSC)
- Microscale Thermophoresis (MST) LabelFree or NT115
- Real-time biosensing: surface plasmon resonance (SPR: Biacore T200 and biolayer interferometry (BLI: Octet Red 384)
- Static multi-angle light scattering (SLS, MALS)
- Taylor dispersion and viscometry
- Differential Scanning Fluorimetry (NanoDSF)
The PFBMI also provides privileged access to instrumentation installed in other Institut Pasteur research units or in the Synchrotron SOLEIL:
- Electrophoretic mobility
- Infrared spectroscopy (FTIR)
- Small-Angle X-ray Scattering (SAXS): SOLEIL SWING beamline
The PFBMI initiated in 2014 a new pan European network of molecular-scale biophysics core facilities, infrastructures and resource laboratories: ARBRE-MOBIEU (Association of Resources for Biophysical Research in Europe / Molecular Biophysics in Europe). Since 2016, ARBRE-MOBIEU is a COST Action, coordinated by P. England.