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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.

About

The “Molecular Biophysics” facility (french acronym: Plate Forme de Biophysique MoleculaIre, PFBMI) federates an ensemble of state-of-the-art instrumentation for the in vitro molecular-scale characterization of biological macromolecules, together with well established technical and methodological expertise. The mission of the PFBMI is to provide an added value to a great variety of biological projects, enabling the characterization of the intrinsic properties of macromolecules and their assemblies (size, shape, folding, stability auto-association,…) and of the interactions in which they are involved (stoichiometry, thermodynamic and kinetic parameters, …).

PFBMI equipments are available for the entire scientific community, in France and abroad, but a priority is given to teams from Institut Pasteur and the Institut Pasteur International Network.

Fields of expertise of the PFBMI :

Quality control (QC) of purified proteins :

This new service allows you to obtain a full qualitative analysis (QC) of your purified proteins and to help you to identify conditions in which these proteins are best behaved.

The basic analysis will provide you with information about the purity, identity, integrity and homogeneity of your proteins. This will be done using three methods: intact mass spectrometry, UV/Visible spectroscopy and Dynamic Light Scattering.

If needed, we can also provide an electrophoresis profile and a SEC chromatogram of your samples.

Optional supplementary analysis:

  • Buffer optimisation: the stability and the solubility of protein samples can be tested in several buffers with different pH and salt compositions.
  • Sample storage optimization: best buffer conditions for storage at 4 to -20°C or freeze drying….
  • “Lot to lot” consistency

For more information, please send an email to qcprotein@pasteur.fr

Technologies available at the PFBMI :

The PFBMI also provides privileged access to instrumentation installed in other Institut Pasteur research units or in the Synchrotron SOLEIL:

  • Electrophoretic mobility
  • Infrared spectroscopy (FTIR)
  • Small-Angle X-ray Scattering (SAXS): SOLEIL SWING beamline

To submit a project to the PFBMI, please send an e-mail to “biophysique@pasteur.fr” together with a completed submission form. After having discussed with the PFBMI team about the project feasibility and the appropriate experimental strategy, you will be required to complete a “sample information form“.

Pricing : services are charged according to the joined chart

The PFBMI  initiated in 2014 a new pan European network of molecular-scale biophysics core facilities, infrastructures and resource laboratories: ARBRE-MOBIEU (Association of Resources for Biophysical Research in Europe / Molecular Biophysics in Europe). Since 2016, ARBRE-MOBIEU is a COST Action, coordinated by P. England.

Projects

Services

Thermodynamic stability of macromolecules

Circular dichroism (CD) spectroscopy allows the determination of secondary and tertiary structures, the analysis of conformational changes and the measure of thermal and chemical stability of folding. Differential Scanning Calorimetry (DSC) gives access to […]

Thermodynamical stability of macromolecules

Circular dichroism (CD) spectroscopy allows the determination of secondary and tertiary structures, the analyse of conformational changes, the kinetic of folding and the measure of thermal and chemical stability of folding. Differential Scanning Calorimeter (DSC) […]

Secondary and tertiary structures of macromolecules

Circular dichroism (CD), allows the measurement of secondary and tertiary structures, the analyse of conformational changes, the kinetic of folding and the thermal and chemical stability of folding Fluorescence spectroscopy  give access to the analysis […]

Size and Shape of Macromolecules and their assemblies

PFBMI project submission form Dynamic Light Scattering (cuvette or microplaque) allows to quantify the diffusion speed of macromolecules in solution and than to deduce their hydrodynamic radius.   Static Light Scattering and viscosimetry give […]

Stoechiometry of Macromolecular Assembly

Static Light Scattering allow to measure the mass and intrinsic viscosity of macromolecules Analytical Ultracentrifugation give access to the oligomerization state and the stoechiometry of the assemblies  

Equipments

Differential Scanning Fluorimetry NanoDSF • Nanotemper • Prometheus NT 48

NanoDSF measures the thermal denaturation of proteins by following the modification of the  environment of their fluorescent aminoacids Tryptophanes and Tyrosines . Prometheus instrument can perform fast and sensitive simultaneous analyses of 48 distinct […]

Infrared spectroscopy (FTIR) • Jasco • 6100

Fourier Transform Infra Red (FTIR) spectroscopy allows to analyze the secondary structure of macromolecules by measuring absorption frequencies of some specific chemical groups (notably, for proteins, that of the amide bond). Informations given by FTIR are […]

Dynamic Light Scattering (DLS) • Wyatt • Dynapro Plate Reader

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a DynaPro […]

Microscale Thermophoresis (MST) NT115 • Nanotemper • NT115

Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]

Microcalorimetry (DSC: Differential Scanning Calorimetry) • Malvern • CAP-DSC

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. The Capillary Differential scanning calorimeter (CAP-DSC, Malvern) is principally dedicated to the study of the thermal stability of […]

Isothermal Titration Microcalorimetry (ITC) • Malvern • ITC200

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200) are principally dedicated to the study of molecular interactions. By measuring the […]

Analytical ultracentrifugation • Beckman-Coulter • ProteomeLab XL-I

The PFBMI has a ProteomeLab XL-I (Beckman-Coulter) analytical  ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, […]

Fluorescence Spectroscopy – Stopped Flow • TgK Scientific • KinetAsyst SF61-DX2

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Fluorescence Spectroscopy • Photon Technology International • Quantamaster QM4CW

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Microscale Thermophoresis (MST) LabelFree • Nanotemper • Monolith LabelFree

Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]

Surface Plasmon Resonance Proteon XPR36 • Biorad • Proteon XPR36

The PFBMI has two microfluidic SPR instruments (Biacore T200, GE Healthcare, and ProteOn XPR36, BioRad) that allow the label-free analysis of molecular interactions in real time. To that avail, one of the interaction partners […]

Surface Plasmon Resonance Biacore T200 • GE Healthcare • Biacore T200

The PFBMI has two microfluidic SPR instruments (Biacore T200, GE Healthcare, and ProteOn XPR36, BioRad) that allow the label-free analysis of molecular interactions in real time. To that avail, one of the interaction partners […]

Microcalorimetry – ITC • Malvern • VP-ITC

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200), principally dedicated to the study of molecular interactions. By measuring the reaction […]

Static Light Scattering and viscometry • Viscotek • TDA 302

The intensity of light scattered by a solution is directly proportional to the average molecular weight and to the concentration of its components. The PFBMI has a multiple detector (Viscotek TDAmax, Malvern), coupled to […]

Dynamic Light Scattering • Wyatt • Dynapro MS800

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a cuvette […]

Circular Dichroism • AVIV • 215

The PFBMI has an Aviv 215 instrument with accessories for the measurement of total fluorescence and to perform titrations. Studying macromolecules (and in particular proteins) by circular dichroism allows to obtain information about their […]

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