Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share

About

The Mass Spectrometry for Biology Utechs is  a mixed Institut Pasteur/CNRS Unit  (USR 2000) and is headed by Julia Chamot-Rooke (DR1 CNRS). The Unit includes the Pasteur proteomics platform and a research group, dedicated to the development of structural mass spectrometry. The Unit is  part of the C2RT (Center or Technological Research and Ressources) and of the Structural Biology and Chemistry department. It is IBISA labeled since 2012 and ISO 9001:2015 certified since April 2018.

The platform, headed by M. Matondo, provides service and collaboration in bottom-up proteomics. It is open to external users.

The research group develops four main axes:
Top-down proteomics is an emerging technology based on the analysis of intact proteins using very high-resolution mass spectrometry. It provides the highest molecular precision for analyzing primary structures and determining post-translational modifications by examining proteins in their intact state, leading to more straightforward and reliable results than the classical bottom-up approach based on protein enzymatic digestion. Top-down proteomics holds great promise in clinical research and in particular in clinical microbiology, a major research axis of the unit.
Cross-linking combined to mass spectrometry is a powerful approach to address both the structure and composition of protein complexes. To improve the crucial stage of detection and characterization of cross-linked peptides, we have developed a new generation of cross-linking agents based on click-chemistry.
Native MS allows the structural investigation of protein complexes with the sensitivity, speed, unlimited mass range and accuracy of mass spectrometry. The exact mass of the proteins can be obtained, as well as the stoichiometry of the assembly, its conformation and topological arrangement of the subunits within the complex.
HDX (Hydrogen Deuterium Exchange) MS is based on the different exchange rates of hydrogens in proteins. Exchange rates are a function of solvent accessibility and hydrogen bonding. HDX MS can be used to monitor conformational changes in proteins, characterize protein folding pathways or protein-protein interactions. HDX MS is also widely used for epitope mapping.

For more information on these different topics, please contact Julia Chamot-Rooke

 

Projects

Transversal Project

Equipments

Orbitrap Fusion Lumos Mass Spectrometer • Thermo Fisher • Orbitrap Tribrid Fusion Lumos

 An Orbitrap Fusion has been installed in 2013 and upgraded in Fusion Lumos in 2016. The instrument is equipped with ETD and used either with a nanoUPLC Dionex for online fragmentation or with a Nanomate for […]

Synapt Mass Spectrometer • Waters • Synapt G2-Si HDMS

The Synapt G2-Si HDMS (with ETD and Ion Mobility) is equipped with a nanoAcquity UPLC and the HDX manager. It was installed in 2013. In 2015, a SID cell (Surface Induced Dissociation) has been installed […]

Orbitrap Velos Mass Spectrometer • Thermo Fisher • LTQ-Velos Orbitrap

The LTQ-Velos Orbitrap is equipped with a U3000 nanoLC (Dionex) and ETD. It is mainly used for the analysis of simple mixtures of proteins after trypsin digestion or PTM analysis using its ETD capabilities. 

Orbitrap Q-Exactive Mass Spectrometer • Thermo Fisher • Q-Exactive Plus & Q-Exactive HF

The Q-Exactive Plus and Q-Exactive HF are equipped with an easy-nLC (Proxeon) and have been installed in 2014/2015. They are used for: Protein identification (Data Dependent and Data Independent Analyses) Post translational modification (PTM) analysis […]

Fundings

Publications

Download

theme 1

Contact

Phone: +33 1 40 61 38 59 Email: julia.chamot-rooke@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France