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About

The proteomics facility, headed by Mariette Matondo, is part of the Mass Spectrometry for Biology  Utechs (MSBio)  directed by Julia Chamot-Rooke. The overall objective of the platform is to develop innovative bottom-up proteomics strategies to address protein function, regulation and dynamic interactions, in particular in the context of infectious diseases. Our collaborators benefit from the expertise of the staff, as well as state-of-the-art instrumentation, including the latest generation of high speed, high-resolution mass spectrometers (Orbitrap), and bioinformatics tools. The proteomics analyses provided by the platform include:

  • Identification of low abundant proteins in complex biological matrices
  • Advanced quantitative proteomic strategies
  • Characterization of post-translational modifications (PTMs)
  • Analysis and dynamics of protein complexes
  • Top-Down Proteomics to identify proteoforms

Analyses can be performed either as research collaboration or service. Prior to starting the project, an experimental design is defined and the staff provides advice on sample preparation. Results are further provided after a bioinformatic analysis of the data.

User Committee members : Marco Bellinzoni, Serge Bonnefoy, Valérie Bouchez,Filipe Carvalho,Ludovic Deriano , Olivier Dussurget, Marie Flamand, Thierry Fontaine,Magdalena Gil-Tarán , Guilhem Janbon,Pierre Alexandr Kaminski, Pascale Pescher , Cosmin Saveanu,  Timothy Wai.

Steering committee members : Philippe Bastin , Pascale Cossart, Jean-Michel CAMADRO (IJM), Erick DUFOURCQ (CNRS), Ivo Gomperts Boneca, Cosmin Saveanu.

How to work with us / How to apply for support

For project proposals, please complete this form and send it to proteomics@pasteur.fr.

If you have any  suggestion on how we can improve our service,  please do not hesitate to contact us: Reclamation / Suggestions

Open Desk

On Monday at 11.00 am.

Satisfaction Surveys

The following files link to the analysis of the surveys

The proteomic faciltiy is certified ISO 9001

Practical Information

We are part of the C2RT (Center for Technological Resources and Research), a center gathering the Technology and Service units (UTechS) and the technological core facilities of the campus. As such we follow the common guidelines and good practices.

If you did not find what you were looking for, please check out the C2RT booklet

Services

Prices and Form

Prices are indicative and could be subject to change. This prices list  was updated in July 2014. For project proposals, please complete this form and send to proteomics@pasteur.fr    

Equipments

Orbitrap Q-Exactive HF Mass Spectrometer • Thermo Fisher • Orbitrap Q Exactive™ HF

The Orbitrap Q-Exactive HF is equipped with an easy-nLC (Proxeon) and has been installed in 2014/2015. It is used for: Protein identification (Data Dependent and Data Independent Analyses) Post translational modification (PTM) analysis Quantitative […]

Orbitrap Velos Pro Mass Spectrometer • Thermo Fisher • LTQ-Velos Orbitrap

The LTQ-Velos Orbitrap is equipped with a U3000 nanoLC (Dionex) and ETD. It is mainly used for the analysis of simple mixtures of proteins after trypsin digestion or PTM analysis using its ETD capabilities.

Orbitrap Q-Exactive Plus Mass Spectrometer • Thermo Fisher • Q-Exactive Plus

The Q-Exactive Plus is equipped with an easy-nLC (Proxeon) and has been installed in 2014/2015. It is used for: Protein identification (Data Dependent and Data Independent Analyses) Post translational modification (PTM) analysis Quantitative proteomics […]

Former Members

2000
2000
Name
Position
2015
2020
Sahra Ouarti
Technician
2015
2020
Véronique Hourdel
Research Engineer
2015
2020
Nesrine El Omrani
Graduate Student
2015
2020
Maxime Bellami
Graduate Student

Projects

Transversal Project

Fundings

Partners

Publications

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Contact

Phone:+33 1 44389388 Email: proteomics@pasteur.fr Proteomics Platform,  Mass Spectrometry for Biology unit  (MSBio) Institut Pasteur Centre François Jacob – 2nd floor 28 Rue du Docteur Roux 75015, Paris France