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  • Clinical Research Nurse
  • Clinician Researcher
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  • Master Student
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About

The proteomics facility, headed by Mariette Matondo, is part of the Mass Spectrometry for Biology  Utechs (MSBio)  directed by Julia Chamot-Rooke. The overall objective of the platform is to develop innovative bottom-up proteomics strategies to address protein function, regulation and dynamic interactions, in particular in the context of infectious diseases. Our collaborators benefit from the expertise of the staff, as well as state-of-the-art instrumentation, including the latest generation of high speed, high-resolution mass spectrometers (Orbitrap), and bioinformatics tools. The proteomics analyses provided by the platform include:

  • Identification of low abundant proteins in complex biological matrices
  • Advanced quantitative proteomic strategies
  • Characterization of post-translational modifications (PTMs)
  • Analysis and dynamics of protein complexes
  • Top-Down Proteomics to identify proteoforms

Analyses can be performed either as research collaboration or service. Prior to starting the project, an experimental design is defined and the staff provides advice on sample preparation. Results are further provided after a bioinformatic analysis of the data.

Please contact Mariette Matondo : mariette.matondo@pasteur.fr for more information

User Committee members : Marco Bellinzoni, Serge BonnefoyChristel Brou, Alexandre Chenal, Ludovic DerianoOlivier Dussurget , Simonetta Gribaldo, Guilhem Janbon,Pierre Alexandr Kaminski, Pierre-Lê BuryChloé Lescale ,Pascale Pescher , Cosmin Saveanu,  Timothy Wai

Steering committee members : Sandrine SAGAN (CNRS), Cosmin Saveanu, Joëlle VINH (ESPCI)

How to work with us / How to apply for support

For project proposals, please submit your project request here on PPMS (stratocore): for the facility use MSBio Core Facilty Request Form : https://ppms.eu/pasteur/req/?pf=29&project=true&form=68. Additionally, you can complete this form and send it to proteomics@pasteur.fr.

If you have any  suggestion on how we can improve our service,  please do not hesitate to contact us: Reclamation / Suggestions

Open Desk

On Tuesday at 10.30 am. Please contact Mariette Matondo  at proteomics@pasteur.fr

Satisfaction Surveys

The following files link to the analysis of the surveys

The proteomic faciltiy is certified ISO 9001

The proteomics facility is IBisA labelled

Practical Information

We are part of the C2RT (Center for Technological Resources and Research), a center gathering the Technology and Service units (UTechS) and the technological core facilities of the campus. As such we follow the common guidelines and good practices

If you did not find what you were looking for, please check out the DT Brochure

Click here to obtain the map

Services

Project request and Prices

For project proposals, please submit your project request here on PPMS (stratocore): for the facility use MSBio Core Facilty Request Form : https://ppms.eu/pasteur/req/?pf=29&project=true&form=68. Additionally, you can complete this form and send it to proteomics@pasteur.fr. […]

Equipments

Orbitrap Eclipse Tribrid Mass Spectrometer • Thermo Fisher • Orbitrap Eclipse™ Tribrid™ Mass Spectrometer

The Orbitrap Eclipse™ Tribrid™ mass spectrometer is cuppled witrh Neo Vanquish or an easy-nLC (Proxeon) and has been installed in January 2021. It is used for: – TMT quantification – Data Independent Acquisition (DIA) […]

Orbitrap Q-Exactive HF Mass Spectrometer • Thermo Fisher • Orbitrap Q Exactive™ HF

The Orbitrap Q-Exactive HF is equipped with an easy-nLC (Proxeon) and has been installed in 2014/2015. It is used for: Protein identification (Data Dependent and Data Independent Analyses) Post translational modification (PTM) analysis Quantitative […]

Orbitrap Fusion Lumos Mass Spectrometer • Thermo Fisher • Orbitrap Tribrid Fusion Lumos

An Orbitrap Fusion has been installed in 2013 and upgraded in Fusion Lumos in 2016. The instrument is equipped with ETD and used either with a nanoUPLC Dionex for online fragmentation or with a Nanomate for […]

Orbitrap Velos Pro Mass Spectrometer • Thermo Fisher • LTQ-Velos Orbitrap

The LTQ-Velos Orbitrap is equipped with a U3000 nanoLC (Dionex) and ETD. It is mainly used for the analysis of simple mixtures of proteins after trypsin digestion or PTM analysis using its ETD capabilities.

Orbitrap Q-Exactive Plus Mass Spectrometer • Thermo Fisher • Q-Exactive Plus

The Q-Exactive Plus is equipped with an easy-nLC (Proxeon) and has been installed in 2014/2015. It is used for: Protein identification (Data Dependent and Data Independent Analyses) Post translational modification (PTM) analysis Quantitative proteomics […]

Former Members

2000
2000
Name
Position
2015
2020
Sahra Ouarti
Technician
2015
2020
Véronique Hourdel
Research Engineer
2015
2020
Nesrine El Omrani
Graduate Student
2015
2020
Maxime Bellami
Graduate Student
2020
2021
Elias Kotbachi
Master student
2013
2022
Thibault Chaze
Research Engineer
2022
2023
Rita Azevedo
Research Engineer

Projects

Transversal Project

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Contact

Phone: Email: proteomics@pasteur.fr Proteomics Platform, Structural Mass Spectrometry and Proteomics Unit Institut Pasteur Centre François Jacob – 2nd floor 28 Rue du Docteur Roux 75015, Paris France