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Scientific Fields
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About

The proteomics facility, managed by Mariette Matondo, is part of the Structural Mass Spectrometry and Proteomics Unit directed by Julia Chamot-Rooke. The overall objective of the platform is to develop innovative bottom-up proteomics strategies to address protein function, regulation and dynamic interactions, in particular in the context of infectious diseases. Our collaborators benefit from the expertise of the staff, as well as state-of-the-art instrumentation, including the latest generation of high speed, high-resolution mass spectrometers (Orbitrap), and bioinformatics tools. The proteomics analyses provided by the platform include:

  • Identification of low abundant proteins in complex biological matrices
  • Advanced quantitative proteomic strategies
  • Characterization of post-translational modifications (PTMs)
  • Analysis and dynamics of protein complexes

Analyses can be performed either as research collaboration or service. Prior to starting the project, an experimental design is defined and the staff provides advice on sample preparation. Results are further provided after a bioinformatic analysis of the data.

For project proposals, please complete this form and send to proteomics@pasteur.fr

 

Events

Projects

Services

Prices and Form

Prices are indicative and could be subject to change. This prices list  was updated in July 2014.For project proposals, please complete this form and send to proteomics@pasteur.fr  

Equipments

Orbitrap Velos Mass Spectrometer • Thermo Fisher • LTQ-Velos Orbitrap

The LTQ-Velos Orbitrap is equipped with a U3000 nanoLC (Dionex) and ETD. It is mainly used for the analysis of simple mixtures of proteins after trypsin digestion or PTM analysis using its ETD capabilities. 

Orbitrap Q-Exactive Mass Spectrometer • Thermo Fisher • Q-Exactive Plus & Q-Exactive HF

The Q-Exactive Plus and Q-Exactive HF are equipped with an easy-nLC (Proxeon) and have been installed in 2014/2015. They are used for:Protein identification (Data Dependent and Data Independent Analyses)Post translational modification (PTM) analysisQuantitative proteomicsTargeted quantification

Publications