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About

Several systems are available at the imaging Facility at the Hearing institute: Confocals, Spining disk, electronic microscope and fluorescence microscopes. These instruments allows observing the sample at different levels, from the entire organoid to the details of intracellular structures (~150nm of lateral resolution in optical microscopy and ~10nm resolution in electronic microscopy). In collaboration with the imaging facility (Imagopole) and the imaging analysis Hub of Pasteur Institute, we offer support in the imaging acquiring process as well as the treatment, analysis and interpretation of images.

Equipments

Epi-fluorescence Microscope • Zeiss • Axiovert

Epi-fluorescence microscope Axiovert Excitation: 375nm/490nm/550nm/633nm Cubes : DAPI/GFP/RFP/Cy5 CMOS Camera Objectives : 10x/03 PH1 WD :5.1mm (Contraste de phase) 20x/0.75 40x/0.75 63x/1.4 Oil

STED microscope • Aberrior Instruments • Stedycon

Confocal scanning microscope with 4 laser lines equiped with a STED module that allows up to 30 nm lateral spatial resolution at two colors (orange and red). It is really simple to use but […]

Spinning-disk Microscope • Gataca • W1

Scanning microscope that allows the acquisition of images with optical sectioning with an extended field of view at very high speed and low photo-toxicity. Equipped with a super-resolution module it allows acquiring images with […]

Confocal Nikon A1HD25 • Nikon • A1HD25

Scanning microscope that allows the acquisition of images with optical sectioning and an extended field of view. Equipped with resonant scanners it is a high speed and low photo-toxicity microscope. It is the ideal […]

Confocal Zeiss LSM 700 • Zeiss • LSM 700

Scanning microscope that allows the acquisition of images with optical sectioning. Suitable for fixed samples showing low expression. Excitation: 405nm/488nm/561nm/633nm Objectives : 5x N0.16 10x NA0.3 20x NA 0.8 25x 0.8W 63x NA1.4 Oil

Confocal Zeiss LSM 900 Airyscan • Zeiss • LSM 900 Airyscan

Scanning microscope that allows the acquisition of images with optical sectioning. Compatible with fixed and living samples with low levels of expression it uses low excitation intensities to gently imaging your samples in 3D […]

Compact electronic microscope (SEM) • Hitachi • SU1510

System used for the observation of intracellular structures in fixed tissue. Detector SE : 0nm à 30kV (High Vacuum Mode) – 10nm à 3kV (High Vacuum Mode) Detector BSE : 0nm à 30kV (Low Vacuum Mode) […]

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