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About

Electron Microscopy at Ultrastructural BioImaging

The Ultrastructural Bioimaging (UBI) facility at the Institut Pasteur provides state-of-the-art electron microscopy imaging approaches to users from the campus and outside. Samples range from single molecules to whole organisms. They can be observed at the highest resolution possible.

What we do:

Who are we?

13 electron microscopists with expertise covering a broad range of TEM & SEM methodologies.

What we offer:

  • Training
  • Collaboration or Service
  • Implementation & Development of new methods

How to get started:

  • Come to the weekly Open Desk every Monday morning in the hall of F. Jacob building for informal discussion or email us at ubi.all@pasteur.fr
  • Come and tell us more about your project in our weekly team meeting
  • New projects should be requested via our online system PPMS.

All samples with a biosafety level P2/P3 require the submission of a ‘protocol de recherche’ to document the procedure of inactivation.

 

Certifications and networks:

Services

Booking site

UBI is a core facility with a broad mandate that supports a wide community of EM users. UBI offers services and hands-on training across various levels. Autonomous level users are able to book and use facility’s systems independently, while assisted users are followed closely by UBI members. On a basic service status, the facility staff  will prepare, observe samples and provide high quality images. In case of scientific collaborations UBI members will be actively involved to all required steps of the research project, including image processing. Collaborative projects are highly valued by UBI, as, along them, new methods can be implemented and developed in the facility.

Training in Electron Microscopy

UBI offers various hands-on trainings in Electron Microscopy covering a broad range of methods and techniques from sample preparation to imaging and image processing Negative staining (2 sessions x 3h) Sample preparation Ultramicrotomy (conventional […]

Image Processing for EM

  Tomographic reconstruction In electron tomography, the tilt series acquired, by tilting the sample to different angles under the electron beam (usually every 1 or 2 degrees from about −60° to +60°), can then […]

Correlative Light and Electron Microscopy

Correlative Light & Electron Microscopy Correlative Light & Electron Microscopy (CLEM) approaches are methods of choice to image rare or dynamic events at high-resolution by Electron Microscopy (EM).  The functional and dynamic information coming […]

Imaging

    Transmission electron microscopy In Transmission Electron Microscopy (TEM) the image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen.  Transmission electron microscopes […]

Sample preparation

  Resin embedding The embedding of a soft biological sample in a plastic resin hardens it enough for thin sectioning. In this way the sample is ready for TEM, TEM-tomography, and FIB-SEM tomography. Classical […]

Single Particle Electron Microscopy

Negative stain and Cryo-electron microscopy is used to provide structural information of macromolecules and their complexes by imaging non-crystalline speciments (single particles) absorbed in electron microscopy grids. Fédry J, Liu Y, Péhau-Arnaudet G, Pei […]

Equipments

Booking site

High Pressure Freezing • Leica • EM ICE

Cryo-fixation has distinct advantages over chemical fixation. It is achieved instantly (within milliseconds) ensuring simultaneous immobilization of all macromolecules present. High Pressure Freezing Currently is the only method to vitrify thicker samples (up to […]

5 • Image Processing Workstations for Electron Microscopy • - • -

All workstations can be booked through the PPMS system. Monet Intel Core i9-7900X CPU@3.30GHz/ 128GB DDR4 2x GTX 1080 Ti OS: CentOS Linux 7.7 Software: CUDA v9.1, CUDA v8.0, CUDA v7.5, UCSF Chimera v1.12, […]

6 • Ultramicrotomes • Leica •

Ultramicrotomes allow easy preparation of semi- and ultra-fine sections of biological samples for Transmission EM or FIB-SEM observations. UBI is equipped with 3 ultramicrotomes for room temperature sectioning and 3 cryo-ultramicrotomes.  

Focused Ion Beam Scanning Electron Microscope • Zeiss • Auriga

  Focused ion beam scanning EM is typically applied to analyze whole cells in 3D. In 2016 Atlas-5 was installed on our Auriga with the goal to establish the automated imaging of serial sections, […]

Scanning Electron Microscope • Jeol • 6700F

SEM is typically applied to image the surface of samples. The Jeol 6700F is a routine SEM that can readily be handled by our users after training. It is also equipped with a cryo-transfer […]

Transmission Electron Microscope • FEI • Tecnai T12

The Ultrastructural BioImaging unit has two transmission EMs operated at 120kV with Lab6-electron source: T12 tomo and T12 cryo. T12 cryo is equipped with a cryo-box for prescreening of samples under cryogenic conditions. It […]

Transmission Electron Cryo Microscope • FEI • Tecnai F20

  The Tecnai F20 is a 200 kV Transmission Electron Microscope (TEM) specifically designed for high resolution data collection and tomography. The Field Emission Gun (FEG) provides high beam intensity and stability. Further structural […]

Automatic Plunge Freezer • Leica • EM GP

Plunge freezing For samples with thickness < few microns, such as isolated particles including proteins, macromolecular complexes, decorated liposomes, viruses, bacteria, eukaryotic cells…. Samples are deposited or grown directly onto EM grids and cryo-fixed […]

CryoCLEM Microscope • Leica • Leica EM Cryo CLEM

Correlative light and electron microscopy (CLEM) can provide localization and identification solutions of rare events/structures in crowded environments. Electron microscopy grids can be mounted on the Leica EM Cryo CLEM microscope. Full grid fluorescent […]

Private: Transmission electron microscopy • Tecnai-T12-FEI •

Classical EM and Tomography at room temperature  

Members

Former Members

2000
2000
Name
Position
Emmanuelle Quemin
Post-doc
Andrea Napoli
PhD Student
Jacomine Krijnse-Locker
Head of Structure
Marie-Christine Prevost
Head of Structure

Projects

Transversal Project

Fundings

Partners

Publications

Download

Covid-19 projects

-Under construction-

Pictures & Media