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About

The PF3PR facility has been established to provide large amounts of high quality purified proteins for researchers from the Institut Pasteur and other academic or non-academic institutions.
We are equipped for small to large scale cultures of eukaryotic or prokaryotic expression systems, e.g. Sf9/Baculovirus, S2 Schneider cells, HEK 293 mammalian cells and E. coli.
We are also involved in developing improved technologies and time-saving advanced protocols for protein production and purification.
We can provide cells and vectors as well as advice and individual training in protein production and purification.

Expression systems
Workflow

For your service requests, you will find the form to fill below.

Download (PDF, 794KB)

Projects

Equipments

Optima L-80 XP Ultracentrifuge

Ultracentrifuge • Beckman Coulter • Optima L-80 XP

The Beckman Optima™ L-80 XP Ultracentrifuge is designed to speed up the discovery process in research involving proteomics, cytomics, or genomics. Dubbed as “the most intelligent ultracentrifuge ever” by Beckman Coulter, the unit is […]

Virus Counter • Virocyt/Sartorius Stedim Biotech • 3100

A novel approach for determining total virus particle concentration that provides precise results in minutes, not days. Get a quantitative picture of viral growth that’s simple, compliant and cost-effective with: Focus: The first and […]

2 • ÄKTA flux • GE Healthcare • s

ÄKTA flux s is a semi-automated tangential flow filtration/cross flow filtration system for concentration and diafiltration as well as cell harvest and clarification.

Fermentor • Eppendorf • Bioflo® 320

The Eppendorf Bioflo® 320 fermentors are intermediate systems ideal for pilot through production applications. Applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Components : Working volume from 0.6 to 5.5 L. Rushton type impeller. 1 TMFC […]

16L-BioFermentor • New Brunswick Scientific / Eppendorf • Bioflo® 510

The New Brunswick™ Bioflo 510 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria in minimum or rich media.

2 • Fermentor • Eppendorf • Bioflo® 310

The Eppendorf Bioflo® 310 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Production of recombinant proteins in E. coli, M. smegmatis, S. cerevisiae, […]

3 • Bioreactors • GE Healthcare • Wave 20/50 EHT / Wave POD II

The disposable WAVE Bioreactor systems were designed based on a non-invasive rocking motion that provides excellent mixing and gas transfer for cell growth. WAVE bioreactor system is a reliable and intuitive cell culture device for […]

ÄKTA flux • GE Healthcare • 6

ÄKTA flux is a versatile cross flow filtration system for sample concentration and diafiltration as well as cell harvest and clarification.

2 • ÄKTA Avant • GE Healthcare • Avant

chromatography systems designed for preparative chromatography for fast and secure developement and scalable methods and processes.

Automated screening Platform • TECAN • Evo 200

The Tecan automated platform is used for rapid cultures screening in μ-24 well micro plate to select the best strain-vector couples and simultaneous evaluation of several culture parameters, as well for analytical protein purifications […]

2 • BiopodF200 microfermenters unit • Fogale Nanotech • Biopod

The BiopodF200 (developed and patented by the PF3PR) allows processes optimization for E.coli, yeast or different microorganisms in 100 ml parallel and independent micro-fermentors. This scale-down step is essential for the reliability of the […]

Cells distrupter • Constant Cells distrupter- Cell D •

The Constant cells distrupter, is used for automatic and continuous breaking cell suspension up to 200 ml per cycle (E.coli, or yeasts). It’s a very efficient and friendly use system that ensures a strict […]

2 • Protein purification equipment • GE Healthcare • AKTA pure

The AKTA pure systems are used for complex or conventional protein purification protocols (affinity resins, Ions Exchange, Gel-Filtration chromatography). The PF3PR purified both soluble proteins and inclusion bodies involving protein renaturation processes.

Fundings

Featured publications

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Contact

E-mail address: PF3PR@pasteur.fr
Address: 25-28 Rue du Docteur Roux
75015, Paris
France