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© Christophe Thomas, Institut Pasteur
Gel d’électrophorèse lors de la purification d’un échantillon protéique

About

The PF3PR facility has been established to provide large amounts of high quality purified proteins for researchers from the Institut Pasteur and other academic or non-academic institutions.
We are equipped for small to large scale cultures of eukaryotic or prokaryotic expression systems, e.g. Sf9/Baculovirus, S2 Schneider cells, HEK 293 mammalian cells and E. coli.
We are also involved in developing improved technologies and time-saving advanced protocols for protein production and purification.
We can provide cells and vectors as well as advice and individual training in protein production and purification.

Steering committee: Nadia Izadi-Pruneyre, Pierre-Jean Corringer, Jessica Vanhomwegen, Florence Pojer (EPFL)

Expression systems
Workflow

For your service requests, please fill the PF3PR application form

As part of the technological resources and research center (C2RT), the PF3PR ensures to follow guidelines and good practices defined by the technology and scientific programs departement (DTPS) in collaboration with the scientific departments.

To follow the latest news from the Platform, you can subscribe to our twitter account
@PF3PR_Pasteur

If you want to have access to the Platform’s equipment, please book through the PPMS booking site

The PF3PR is ISO 9001 certified

To find out more see the Technology Department brochure here.

Services

Booking site

High-scale protein production in prokaryotic expression systems

Large amounts of biomolecules may be needed in some specific projects. Our range of biofermentors coupled with high density culture media allow us to produce higher quantity of bio mass and proteins.

Protein purification

The Platform has several automated chromatographic systems of AKTA type allowing it to purify proteins. We can purify your biomolecules of interest for further experiments like biochemical, structural studies and so on.

Equipments

Booking site
Robot pipetage Integra Assist Plus

Integra Assist Plus • Integra • Assist Plus

INTEGRA has developed the ASSIST PLUS pipetting robot to optimize usual pipetting tasks. This robot automates routine pipetting, which improves reproducibility and eliminates pipetting errors.

Precellys 24 tissue homogenizer • Bertin • Precellys 24

The Precellys 24 can homogenize many different samples in 30 seconds. It possesses a unique automatic tube locking system, a real push-and-play system that makes the Precellys 24 the easiest homogenizer to handle. The […]

DASbox® Mini Bioreactor system • Eppendorf • DASbox®

The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications. It is designed as a 4-fold system with up to twenty-four parallel operating bioreactors. […]

ambr® 15 • Sartorius • ambr® 15

The ambr® 15 cell culture system enables the rapid evaluation of multiple bioreactor cultures at microscale, increasing productivity in cell line development with significant savings on materials and labour. ambr® 15 has been proven […]

Virus Counter • Virocyt/Sartorius Stedim Biotech • 3100

A novel approach for determining total virus particle concentration that provides precise results in minutes, not days. Get a quantitative picture of viral growth that’s simple, compliant and cost-effective with: Focus: The first and […]

2 • ÄKTA flux • GE Healthcare • s

ÄKTA flux s is a semi-automated tangential flow filtration/cross flow filtration system for concentration and diafiltration as well as cell harvest and clarification.

Fermentor • Eppendorf • Bioflo® 320

The Eppendorf Bioflo® 320 fermentors are intermediate systems ideal for pilot through production applications. Applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Components : Working volume from 0.6 to 5.5 L. Rushton type impeller. 1 TMFC […]

16L-BioFermentor • New Brunswick Scientific / Eppendorf • Bioflo® 510

The New Brunswick™ Bioflo 510 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria in minimum or rich media.

2 • Fermentor • Eppendorf • Bioflo® 310

The Eppendorf Bioflo® 310 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Production of recombinant proteins in E. coli, M. smegmatis, S. cerevisiae, […]

3 • Bioreactors • GE Healthcare • Wave 20/50 EHT / Wave POD II

The disposable WAVE Bioreactor systems were designed based on a non-invasive rocking motion that provides excellent mixing and gas transfer for cell growth. WAVE bioreactor system is a reliable and intuitive cell culture device for […]

ÄKTA flux • GE Healthcare • 6

ÄKTA flux is a versatile cross flow filtration system for sample concentration and diafiltration as well as cell harvest and clarification.

2 • ÄKTA Avant • GE Healthcare • Avant

chromatography systems designed for preparative chromatography for fast and secure developement and scalable methods and processes.

Automated screening Platform • TECAN • Evo 200

The Tecan automated platform is used for rapid cultures screening in μ-24 well micro plate to select the best strain-vector couples and simultaneous evaluation of several culture parameters, as well for analytical protein purifications […]

2 • BiopodF200 microfermenters unit • Fogale Nanotech • Biopod

The BiopodF200 (developed and patented by the PF3PR) allows processes optimization for E.coli, yeast or different microorganisms in 100 ml parallel and independent micro-fermentors. This scale-down step is essential for the reliability of the […]

Cells distrupter • Constant Cells distrupter- Cell D •

The Constant cells distrupter, is used for automatic and continuous breaking cell suspension up to 200 ml per cycle (E.coli, or yeasts). It’s a very efficient and friendly use system that ensures a strict […]

3 • ÄKTA pure • GE Healthcare • ÄKTA pure

The AKTA pure systems are used for complex or conventional protein purification protocols (affinity resins, Ions Exchange, Gel-Filtration chromatography). The PF3PR purified both soluble proteins and inclusion bodies involving protein renaturation processes.

Former Members

2000
2000
Name
Position
2003
2012
Vincent Bondet
Research Engineer
2015
2015
Morgane Ropert
Graduate Student
2015
2016
Marion Gransagne
Post-doc
2008
2016
Fabienne Levi-Acobas
Technician
2010
2017
Christine Girard Blanc
Research Engineer
2002
2017
Emmanuel Frachon
Research Engineer
2016
2017
Fiona Maillard
Technician
2005
2017
Marie-Reine Lauret
Lab assistant
2017
2017
Adélie Belin
Graduate Student
2018
2018
Audrey Chamarande
Graduate Student
2019
2019
Souad Benyoucef
Graduate Student
2019
2020
Maud Maréchal
Graduate Student
2021
2022
Loïc Hellio
Research Engineer
1997
2022
Jacques Bellalou
Research Engineer - Deputy Director
2022
2023
Angélique Dalleau
Research Engineer - in training
2023
Christophe Thomas
Research Technician
2022
2023
Anthony Rodrigues
Research Engineer - in training
2024
Lucie Cappuccio
Research Engineer
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Contact

E-mail address: PF3PR@pasteur.fr
Address: 25-28 Rue du Docteur Roux
75015, Paris
France