In solution conformation of the SigmaS subunit of RNA polymerase
There is no tridimentional structure available for free sigma factors. We have recently probed the conformation of SigmaS in solution by analytical ultracentrifugation, small angle X-ray scattering and Hydrogen-Deuterium exchange coupled with mass spectrometry.
Implementation of automated procedures for high-throughput screening cultures and analytical purification of protein
The availability of recombinant proteins remains a bottleneck for many scientific projects. E.coli is the most widely expression system used, but the production of soluble proteins requires the simultaneous assessment and combination of multiple […]
Development of an immunoassay for the diagnosis of porcine cysticercosis
Neurocysticercosis (NCC) caused by Taenia solium, is one of the most important parasitic infections of the central nervous system. Both human and pigs can act as intermediate hosts carrying the larvae stage, i.e. developing […]
Rod-cone protein interactions and therapeutic perspective of retinal degenerations.
Loss of cone photoreceptors in retinitis pigmentosa (RP) leads to complete blindness. The group of Thierry Leveillard at the “Institut de la Vision” showed that injection of the RdCVF protein is a promising therapy […]
Molecular mechanisms of action of sigmaS in bacterial fitness and adaptation to the environment.
In Escherichia coli and Salmonella enterica serovar Typhimurium, the regulatory protein Crl regulates gene expression by increasing the performance of RpoS/σS sigma factor to modify global gene transcription in stationary phase and under stress […]
The Eppendorf Bioflo® 320 fermentors are intermediate systems ideal for pilot through production applications. Applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Components : Working volume from 0.6 to 5.5 L. Rushton type impeller. 1 TMFC […]
The New Brunswick™ Bioflo 510 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Batch or fed batch of E. coli […]
The Eppendorf Bioflo® 310 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Production of recombinant proteins in E. coli, M. smegmatis, S. cerevisiae, […]
The disposable WAVE Bioreactor systems were designed based on a non-invasive rocking motion that provides excellent mixing and gas transfer for cell growth. WAVE bioreactor system is a reliable and intuitive cell culture device for […]
FlexStand benchtop pilot scale systems is compact, sanitary hollow fiber ultrafiltration/microfiltration systems for laboratory through pilot scale applications. This cross flow system is designed for fast, efficient concentration and/or diafiltration of a wide range of biological […]
ÄKTA flux is a versatile cross flow filtration system for sample concentration and diafiltration as well as cell harvest and clarification.
ÄKTAcrossflow is a fully automated cross flow filtration system for process development and it is suitable for filter screening and process optimization at small-scale as well as small-scale processing.
chromatography systems designed for preparative chromatography for fast and secure developement and scalable methods and processes.
The Tecan automated platform is used for rapid cultures screening in μ-24 well micro plate to select the best strain-vector couples and simultaneous evaluation of several culture parameters, as well for analytical protein purifications […]
The BiopodF200 (developed and patented by the PFPR) allows processes optimization for E.coli, yeast or different microorganisms in 100 ml parallel and independent micro-fermentors. This scale-down step is essential for the reliability of the […]
PFPR is equipped with conventional fermenters of 2-L up to 20-L, for cultures of E. coli or yeast in complex or labeled chemically defined media. We have developed an home-made high-density culture medium for […]
The Constant cells distrupter, is used for automatic and continuous breaking cell suspension up to 200 ml per cycle (E.coli, or yeasts). It’s a very efficient and friendly use system that ensures a strict […]
The AKTA pure system and AKTA Express units are used for complex or conventional protein purification protocols (affinity resins, Ions Exchange, Gel-Filtration chromatography). The PFPR purified both soluble proteins and inclusion bodies involving protein […]