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About

The flow cytometry core facility, integrated into the Cytometry & Biomarker UTechS at Institut Pasteur, provides cutting-edge instrumentation, as well as high-level scientific and technical expertise in flow cytometry in general. The core facility is part of the C2RT (Center for Technological Resources and Research), a center gathering the Technology and Service units (UTechS) and the technological core facilities of the campus.

Technological pipelines & New
   Single cell phenotyping and cell sorting are supported by the Cytometry platform.
The CB UTechS of the Institut Pasteur proposes numerous resources for single cell analysis, open to the entire academic and clinical research community.

    High dimensional cytometry pipeline (conventional and spectral Cytometry):Choosing the best marker/dye-combination can make all the difference to your flow cytometry panels. But that selection can sometimes feel more art than science. Our flow cytometry experts can help you find the right balance between epitope expression and fluorochrome brightness, ensuring you’ve got the very best configuration for your application of interest – whatever it may be.
   ♦ Nanocytometry pipeline : subcellular analysis and nano sorting (Nano FCM, MoFlow Astrios).
   ♦ Unsupervised data analysis (FlowJo plugins)

Service & Support
    The platform provides services on cell sorting, flow cytometry analysis, consulting, data analysis, troubleshooting, and training.
    In our core facility, equipment is completely state-of-the-art with :
       ∗ 7 high-speed cell sorters
       ∗ 5 high-end analyzers : (Conventional cytometry & Spectral cytometry)
       ∗ 1 NanoAnalyser
∗ 2 instruments for cell enrichment by magnetic beads : AutoMACS Pro Separator.

We also gather ressources for our users : Cytometry aids and protocols.
We support two software packages for flow cytometry analysis. Both software packages are available as site licenses through individual internet logins and Virtual Machine ( send an email at FlowJo-VM@pasteur.fr)
FlowJo
Kaluza

Training (see :PFC specific training)
Training courses proposed by the flow cytometry core facility are available for Institut Pasteur researchers, as well as external users (academic and industrial).
First, we give courses on the conceptual aspects of flow cytometry (face-to-face or online), then we give practical training on specific instruments.
We propose FlowJO™ and Kaluza trainings to teach researchers how to analyze flow cytometry data in an efficient way. In FlowJo X, the use of plugins can be used for unsupervised analysis (Bioinformatics support is also available at the CB UTechS).
Every year, we also propose a one week Flow Cytometry Course based on  “Fundamental Cytometry”.

Quality management
As we are involved in quality management (ISO9001), we are attentive to the expectations of users. We do not focus only on the technical aspects of the instrumentation, but we make the link between these technical aspects and the scientific questions.

HOW TO ACCESS : The CB UTechS is an open-access facility that welcomes projects and teams from the Institut Pasteur, from the International Network of the Institut Pasteur, from academia, hospitals and industry. In order to obtain access to our technologies and services, submit your project through our electronic project submission tool. Once your project is submitted you can book an Instrument/training (PPMS).

Services

Booking site

Need training? Someone to look over your data? Panel design? Teaching? Help to plan your experiment? Got a new application or developing a new technique and you’d like to set up a collaboration with us?

Contact us (pfc@pasteur.fr) to discuss all aspects of flow cytometry, from feasibility to execution: theory teaching, instrument training, advice with sample preparation are some of the steps on which the cytometry team can help you. We are available for departmental seminars, advanced user training  and other outreach activities (secondary and undergraduate teaching). Engage the experts and ensure that your flow cytometry experimentation is done right, is reproducible and publishable.
Training for automomous use of the equipment
Assistance for using a cytometer
Advice and technical support
Scientific collaborations
Access to different data analysis software
Protocols and Cytometry Tutorials
Cytometry Glossary
Resources

Training @ Teaching

TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]

Use Fees

Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below : Technology Autonomous Assisted Training* Academic / Industry Cytometers […]

Equipments

Booking site

Twice a week, our morning routine at the flow cytometry core facility starts roughly at 9:30, and we basically have 2 hours to set up all the instruments, do the fluidics cleaning and quality control checks, so that we make sure that the instruments are performing well for the researchers that typically arrive around 11:30 to start their experiments.

Cell Sorters
Cytometry staff are highly experienced in multicolor immune cell sorting of human and mouse cells, bacteria, virus, and others sub-cellular particules. We provide support for RNA Seq applications (sorting in bulk or in 384-well plates).
We propose cell sorting instrumentations in BSL1, BSL2 and BSL2+ environments (see individual equipment description).

Cell Analyzers
The Cytometry platform offers a range of conventional and spectral cytometers covering the multitude of applications requested by our researchers. We propose instrumentations in BSL1, BSL2 and BSL2+ environments (see individual equipment description).
Conventional Flow Cytometry
Today, available instruments are designed to detect up to 50 different bio‐markers on an individual cell. Typically these markers are fluorescent antibodies, molecular sensors, or genetically encoded reporters. Our high-parameter flow cytometers are powerful analytical tools that enable scientists to identify and analyze distinct phenotypes in heterogeneous populations.
Spectral Flow Cytometry
Since 2016, we have developed our expertise in spectral cytometry.
For spectral flow cytometry, the “one detector, one marker” paradigm is not true anymore.
Spectral Cytometry is an emerging technology that revolutionizes the cytometry by identifying specific fluorochrome “signatures”and by eliminating need for compensation. These instruments employs a prism array to disperse the collected light over a 32 channel PMT. The measured spectra from single cells are subsequently unmixed by using reference spectra of all used dyes and autofluorescence spectrum. Least Square Fitting algorithms are used to calculate the most accurate fit for all reference spectra, leading to an accurate determination of which dyes are present on each cell and at which intensity. These capabilities allow the detection of really close emission spectra dyes to achieve greater flexibility for panel design and more accurate visualization of results. The sensitivity is increased by removal of auto-fluorescence.

 

 

 

Show the location of equipments Hide location

NanoFCM • NanoFCM Co. Ltd •

The NanoAnalyzer  is a dedicated flow cytometer built specifically for analyzing nano-sized particles such as extracellular vesicles (EVs), viruses, nanoparticles, based on light scattering and fluorescence for the  detection of single virions or EVs […]

2 • Spectral Analysers • Sony •

ID 7000 6 lasers (355nm, 405nm, 488nm, 561nm and 637nm) which allow for full spectral detection of the resulting emission spectral. BSL2+ : P2+ pathogens and blood ID 7000 Optical Configuration SP 6800 3 […]

CytoFlex S – Cytometer • Beckman Coulter •

4  lasers (405nm, 488nm, 561nm, 638nm) and 13 fluorescent detectors. This analyser is under a P2 PSM : P2 pathogens and Blood plate analysis Software : CytExpert CytoFlex S Optical Configuration   

LSR Fortessa – Cytometer • BD •

5 laser (355nm, 405nm, 488nm, 561nm and 640nm) and 18 fluorescent detectors No pathogens – No Blood Software : Diva 8 LSR Fortessa Optical Configuration      BD LSR Fortessa cytometer technical poster showing optical […]

Symphony A5 – Cytometer • BD •

5 laser (355nm, 405nm, 488nm, 561nm and 637nm) and 31 fluorescent detectors No pathogens – No Blood Software : Diva 8 Web site BD FACSymphony : Symphony A5 Flow Cytometer FACSymphony A5 Optical Configuration    […]

Symphony S6 – Cell sorter • BD •

5 lasers (355nm, 405nm, 488nm, 561nm, and 637nm) and 29 fluorescent detectors BSL2+ : P2+ pathogens and blood Nozzle sizes : 70um; 85um; 100um; 130um Sorting : 1 to 6 way for tubes and […]

FACSAria Fusion – Cell sorters • BD •

  The BD FACSAria™ Fusion Flow Cytometer provides sensitivity for multicolor and sorting applications. The fusion of safety, performance and sorting The BD FACSAria™ Fusion Flow Cytometer combines sorting capabilities with best-in-class biosafety expertise […]

3 • FACSAria III – Cell sorters • BD •

BD FACSAriaIII™ sorters Family Those instruments can be used by independent researchers or as an assisted service. We propose access to 3 FACSAria III cell sorters for bulk or single-cell sorting. BD web site : Technical […]

2 • Astrios High Speed Sorter • Beckman Coulter • MoFlo EQ

The MoFlo Astrios is created by Beckman Coulter’s compagny. This is a jet-in-the-air sorter and has 5 or 7 lasers (UV, violet, red, blue and yellow-green) and two light scatter parameter can be measured […]

2 • AutoMACS Pro • Miltenyi Biotec •

Rapid magnetic enrichment or depletion of cell populations Easy cell separation  with the autoMACS® Pro Separator. Reduce your manual handling steps, save time, and count on greater reliability, reproducibility, and standardization. The autoMACS® Pro […]

Software

Transversal Project

Tools

We offer you various tools to optimize your cytometry experiments:
Cytometry Memory Aid poster
Several cytometry protocols (antibody titration, apoptosis, cell cycle, isolation of PBMCs, calcium analysis, membrane permeability, etc…)

Publications

Download

PFC specific training

The flow cytometry facility organized various courses in flow cytometry every year. We offer basic training to users for a range of instruments, assays, and software. Our courses involve the fundamental aspects of flow cytometry and its applications and there are practical sessions. The facility also offers tailor-made training courses.

Registered users can see all training request types in PPMS: https://ppms.eu/pasteur/req/?pf=10&training=true&form=6

Basics of cytometry TRAINING
We provide training for operating cytometers within the Cytometry pf/CBUTechS.
Details: instrument training provided upon request.
Prerequisite: NA
Duration: 2hr of theoretical session, 2 hr of hands on session.
Location: Flow Cytometry pf/ CBUTechS (FJ level 1).
Register: facility users can request training using the PPMS training request form.

 

 CytoFlex S TRAINING
We provide training for operating our 4-laser 13-parameter cytometers.
Details: instrument training provided upon request.
Prerequisite: basics of cytometry training.
Duration: 4hr of theoretical/ hands on session.
Location: Flow Cytometry pf/ CBUTechS (FJ level 1).
Register: facility users can request training using the PPMS training request form.

 

 BD Fortessa/ Symphony A5 TRAINING
We provide training for operating our 5-laser 18 or 30-parameter cytometers.
Details: instrument training provided upon request.
Prerequisite: basics of cytometry training.
Duration: 2hr of theoretical session, 1 day of hands on session.
Location: Flow Cytometry pf/ CBUTechS (FJ level 1).
Register: facility users can request training using the PPMS training request form.

 

 BD AriaIII/ Fusion/Symphony S6 TRAINING
We provide training for operating our 5-laser 18 or 30-parameters cell sorters.
Details: instrument training provided upon request.
Prerequisite: basics of flow cytometry, DIVA software, PMTs & compensation settings, Panel design, experiment set up.
Duration: 2hr of theoretical session, 1 day of hands on session.
Location: Flow Cytometry pf/ CBUTechS (FJ level 1).
Register: facility users can request training using the PPMS training request form.

 

 FlowJO basic TRAINING
We provide training for various kinds of analysis software, including FlowJo analysis of flow cytometry data.
Prerequisite: FlowJo tutorials and online webinars.
Duration: 1 day of hands on session.
Location: Pasteur n°20.
Register: facility users can request training using the PPMS training request form.

 

 FlowJO plugins TRAINING
We provide training for unsupervised analysis by using R packages
Details: instrument training provided upon request.
Prerequisite: flowJO basics.
Duration: 1 day of hands on session.
Location: Pasteur n°20.
Register: facility users can request training using the PPMS training request form.

 

 Kaluza TRAINING
We provide training for various kinds of analysis software, including FlowJo analysis of flow cytometry data.
Prerequisite: FlowJo tutorials and online webinars.
Duration: 1 day of hands on session.
Location: Flow Cytometry pf/ CBUTechS (FJ level 1).
Register: facility users can request training using the PPMS training request form.

 

Contact

Phone: +33 (0)1 45 68 82 31 or +33 (0)1 45 68 85 38

Email: pfc@pasteur.fr

Address: 25-28 Rue du Docteur Roux, 75015 Paris, France