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About

The flow cytometry core facility, integrated into the Cytometry & Biomarker UTechS at Institut Pasteur, provides cutting-edge instrumentation, as well as high-level scientific and technical expertise in flow cytometry in general. The core facility is part of the C2RT (Center for Technological Resources and Research), a center gathering the Technology and Service units (UTechS) and the technological core facilities of the campus.

The C2RT of the Institut Pasteur proposes numerous resources for single cell analysis, open to the entire academic and clinical research community. Single cell phenotyping (flow cytometry) and cell sorting are supported by the Cytometry platform of the CB UTechS .

The services that we provide here go from cell sorting analysis, consulting, data analysis, troubleshooting, and training.

In our core facility, equipment is completely state-of-the-art with : 

Training
Training in the flow cytometry core facility : first, we give courses on the conceptual aspects of flow cytometry (face-to-face or online), then secondly we give practical training on the instruments.

  • The training courses are for Pasteur researchers, but also for external users (academic and industrial).
  • We propose FlowJO™ and Kaluza trainings to teach researchers how to analyze flow cytometry data in an efficient way using Flowjo X, the world’s nr.1 analysis software tool for flow cytometry data, including the use of plugins for unsupervised analysis. Bioinformatics support is also available at the CB UTechS.
  • We gather ressources for our users : Cytometry aids and protocols

Quality management
As we are involved in quality management (ISO9001), we are attentive to the expectations of users. We do not focus only on the technical aspects of the instrumentation, but we make the link between these technical aspects with the scientific questions.
Whenever somebody has a problem with an instrument, we perform diagnostic to help them in an  emergency and we are in close contact with technical service of the different companies for curative interventions.
The flow cytometry core facility/ CBUTechS is here to support the scientists. And it plays a crucial role in scientific research at Institut Pasteur.

HOW TO ACCESS: The CB UTechS an open-access facility that welcomes projects and teams from the Institut Pasteur, from the International Network of the Institut Pasteur, from academia, hospitals and industry. In order to obtain access to our technologies and services, submit your project through our electronic project submission tool. Once your project is submitted you can book an Instrument/training (PPMS).

Services

Booking site

Need training? Someone to look over your data? Panel design? Teaching? Plan your experiment? Got a new application or developing a new technique and you’d like to set up a collaboration with us?

Contact us (pfc@pasteur.fr) to discuss all aspects of flow cytometry, from feasibility to execution, instrument training and theory teaching and advice with sample preparation. We are available for Departmental seminars, advanced user training  and other outreach activities (secondary and undergraduate teaching). Engage the experts and ensure that your flow cytometry experimentation is done right, is reproducible and publishable.

Training for automomous use to the equipment

Assistance in using an cytometer

Advice and technical support

Scientific collaborations

Access to different data analysis software

Protocols and Cytometry Tutorials

Cytometry Glossary

Resources

Training @ Teaching

TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]

Use Fees

Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below: Technology Autonomous Assisted Training* Academic / Industry Cytometers & […]

Equipments

Booking site

Twice a week, our morning routine at the flow cytometry core facility starts roughly, at 9:30, and we basically have 2 hours to set up all the instruments, do the fluidics cleaning and quality control checks, so that we make sure that the instruments are performing well for the researchers that typically arrive around 11:30 and start their experiments.

Cell sorting

Cytometry staff are highly experienced in multicolor immune cell sorting of human and mouse cells, bacteria, virus, and others sub cellular particules. We provide upport for for RNA Seq applications (sorting in bulk or in P384).
We propose cell sorting instrumentation in BSL1, BSL2 and BSL2+ environments (see individual equipment description)

Cell Analysers

The Cytometry platform offer a range of conventional and spectral cytometers catering to the multitude of applications demanded by our researchers. We instrumentation in BSL1, BSL2 and BSL2+ environments (see individual equipment description)

  • Conventional Flow Cytometry
    Today, instruments are available designed to detect up to 50 different bio‐markers on an individual cell. Typically these markers are fluorescently tagged antibodies, molecular sensors, as well as genetically encoded reporters. Our high parameters flow cytometers are a powerful analytical tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations.
  • Spectral Flow cytometry
    Since 2016, we have developed our expertise in spectral cytometry.
    For spectral flow cytometry, the “one detector, one marker” paradigm is changed.
    Emerging technology that revolutionizes the cytometry by identifying specific fluorochrome “signatures”and by eliminating need for compensation. These instruments employs a prism array to disperse the collected light over a 32 channel PMT. The measured spectra from single cells are subsequently unmixed by using reference spectra of all used dyes and the autofluorescence spectrum. Least Square Fitting algorithms are used to calculate the most accurate fit for all reference spectra, leading to an accurate determination of which dyes are present on each cell and at which intensity. These capabilities allow the detection of really close emission spectra dyes to achieve greater flexibility for panel design and more accurate visualization of results. The sensitivity is increased by removal of auto-fluorescence.

 

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2 • Spectral Analysers • Sony •

ID 7000 6 lasers (355nm, 405nm, 488nm, 561nm and 637nm) which allow for full spectral detection of the resulting emission spectral. BSL2+ : P2+ pathogens and blood ID 7000 Optical Configuration SP 6800 3 […]

CytoFlex S – Cytometer • Beckman Coulter •

4  lasers (405nm, 488 nm, 561 nm, 638 nm) and 13 fluorescent detectors. This analyser is under a P2 PSM : P2 pathogens and Blood plate analysis Software : Summit CytoFlex S Optical Configuration  […]

LSR Fortessa – Cytometer • BD •

5 laser (355nm 405nm, 488nm, 561nm and 640nm) and 18 fluorescent detectors No pathogens – No Blood Software : Diva 8 LSR Fortessa Optical Configuration      BD LSR Fortessa cytometer technical poster showing optical […]

Symphony A5 – Cytometer • BD •

5 laser (355nm 405nm, 488nm, 561nm and 637nm) and 31 fluorescent detectors No pathogens – No Blood Software : Diva 8 Web site BD FACSymphony : Symphony A5 Flow Cytometer FACSymphony A5 Optical Configuration    […]

Symphony S6 – Cell sorter • BD •

5 lasers (355nm, 405nm, 488nm, 561, and 637nm) and 28 fluorescent detectors BSL2+ : P2+ pathogens and blood Nozzle sizes : 70um; 85um; 100um; 130um Sorting : 1 to 6 way for tubes and […]

2 • FACSAria Fusion – Cell sorters • BD •

  The BD FACSAria™ Fusion Flow Cytometer provides sensitivity for multicolor and sorting applications. The fusion of safety, performance and sorting The BD FACSAria™ Fusion Flow Cytometer combines sorting capabilities with best-in-class biosafety expertise […]

3 • FACSAria III – Cell sorters • BD •

BD FACSAriaIII™ sorters Family Those instruments can be used by independent researchers or as an assisted service. We propose access to 3 FACSAria III cell sorters for bulk or single-cell sorting. BD web site : Technical […]

2 • Astrios High Speed Sorter • Beckman Coulter • MoFlo EQ

The MoFlo Astrios is created by Beckman Coulter’s compagny. This is a jet-in-the-air sorter and has 5 or 7 lasers (UV, violet, red, blue and yellow-green) and two light scatter parameter can be measured […]

SP6800 – Spectral cytometer • Sony •

Spectral analyzer SP6800 – Sony Emerging technology that revolutionizes the cytometry by identifying specific fluorochrome “signatures”and by eliminating need for compensation. Equipped by 3 lasers (488nm, 635nm and 405nm), SP6800 collects the entire fluorescence spectrum […]

2 • AutoMACS Pro • Miltenyi Biotec •

Rapid magnetic enrichment or depletion of cell populations Easy cell separation  with the autoMACS® Pro Separator. Reduce your manual handling steps, save time, and count on greater reliability, reproducibility, and standardization. The autoMACS® Pro […]

Transversal Project

Tools

We offer you various tools to optimize your cytometry experiments :

  • cytometry memory aid poster
  • different cytometry protocols (antibody titration, apoptosis, cell cycle, isolation of PBMCs, calcium analysis, membrane permeability, etc.)

Publications

Download

Contact

Phone: 00 33 (0)1 45 68 82 31 or 0 33 (0)1 45 68 85 38

Email: pfc@pasteur.fr

Address 25-28 Rue du Docteur Roux 75015, Paris France