Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share
© Biologie structurale et chimie
Structure du domaine en doigt de zinc de la protéine NEMO, déterminée par Résonance magnétique nucléaire (RMN). Cette protéine jouant un rôle dans des maladies (cancer, inflammation), les connaissances acquises sur sa structure offrent de précieuses informations sur sa fonction.

About

Chemogenomic and biological screening are important steps in precision medicine as well as in the discovery of new medicines but also in dissecting molecular pathways and biological networks.

PF-CCB supports researchers in improving the efficiency of their target-based (HTS) and phenotypic (HCS) screening bioassays, in identifying active substances and genetic modulators of function (siRNA, miRNA, gRNA) and in using core technologies typically applied to miniaturization and automation of bioassays.

We also support drug repositioning via an unique updated FDA-approved library and develop innovative methods (i) to screen for agents that alter a subtle change of phenotype in a single cell (2D) or organ (3D), (ii) to isolate protein-protein interaction inhibitors using label free technology, (iii) to carry out reverse and normal transfection of genetic modulators using acoustic liquid dispensing technology, and (iv) to identify protein targets of your hits (target deconvolution).

What can we do for you ?

We assist research teams with the development of robust target-based (HTS) and phenotypic (HCS) assays, and provide high quality Hits (small molecules, peptides and natural compounds). For HCS, phenotypic analysis can be carried out either with a conventional automated microscope (IX83, Olympus) or the new microscope Stellarvision that uses structured illumination and Synthetic Aperture Optics (SAO). For HTS screening assay technologies, we have the ability to support cellular and biochemical assays using absorbance, fluorescence anisotropy, time-resolved fluorescence, time-resolved fluorescence resonance energy transfer, AlphaScreen and luminescence. We make also available the Octet HTX instrument, which enables high throughput label-free detection via Biolayer interferometry technology (fortebio, Pall life sciences). With our liquid acoustic dispenser (Echo550, labcyte), we can very easily reformate multiwell plates for you (384 and 1536 wells), offer cherry-picking service and prepare any daughter plates from various biological and chemical collections. Finally, we also provide strong supports for developing drug candidates (hit to lead and lead optimization), especially for some promising projects with a high therapeutic index.

Our expertise includes:

  • Microscopy/phenotypic-based screening assay with high resolution imaging (HCS)
  • Target-based screening assay (HTS)
  • Protein-protein and RNA-protein interaction inhibitors
  • Medicinal chemistry
  • Target deconvolution
  • Various functional and chemical libraries including small molecules, peptide, antibody-like protein and siRNA (ubiquitome)

How can you apply for support ?

A request for support starts by sending an email to pfccb@pasteur.fr. You will be asked to give us an overview of your project at first and then meet in person to give us any details so we can figure out together the best way to develop your HTS or HCS bioassay. We can assist you to become autonomous on some automated equipment, help you during every steps of the screening process and can perform for you a medium and large screening campaign.

Transversal Project

Equipments

Show the location of equipments Hide location

Array

Echo Liquid Handler System • Labcyte • Echo 550

The Echo 550 instrument enables non-contact liquid handling using sound energy, high speed, contamination-free transfer of cDNA, siRNA vectors, proteins and crystallography reagents – in nanoliter increments. Echo Plate Reformat software offers a variety […]

Octet Instrument • ForteBio • Octet HTX System

The Octet HTX instrument monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed. The system’s ability to read 8, 16, 32, 48 or 96 wells […]

Microscope • Olympus • IX83

The fully motorized IX83 is the most advanced member of the IX3 series of inverted imaging systems and offers a new level of application flexibility. Choose from the one-deck system with a low, ergonomic […]

Microscope • Optical Biosystems • StellarVision

Target-based, cell-based and phenotypic/microscopy screening assay have numerous advantages and disadvantages and an important challenge in the near future will be to effectively incorporate new screening technologies into phenotypic screening approaches since the traditional […]

Plate Reader • Wyatt Technology • DynaPro Plate Reader II

For small molecules, the DynaPro Plate Reader II can easily detect compound aggregates and aggregation inducers which often result from High-Throughput Screening (HTS) campaign. These primary Hits are often considered as false-positives with poor […]

Fundings

Publications

Download

Contact

Phone: +33 (0)1 40 61 38 10 Email: pfccb@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France