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  • PhD Student
  • Physician
  • Post-doc
  • Prize
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  • Research Associate
  • Research Engineer
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  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
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  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
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© Biologie structurale et chimie
Structure du domaine en doigt de zinc de la protéine NEMO, déterminée par Résonance magnétique nucléaire (RMN). Cette protéine jouant un rôle dans des maladies (cancer, inflammation), les connaissances acquises sur sa structure offrent de précieuses informations sur sa fonction.

About

Chemogenomic and biological screening are important steps in the discovery of new medicines as well as in precision medicine but also in dissecting molecular pathways and biological networks.

What we do:
Target-based (High Throughput Screening, HTS) and phenotypic (High Content Screening, HCS) screening assays are powerful tools to identify active chemical substances and genetic modulators of function (siRNA, miRNA, gRNA) providing starting points for drug design or for understanding biological processes. Using liquid handling devices, sensitive detectors and data processing software, HTS/HCS allow a researcher to rapidly test a large number of samples.

Typical screening cascade:


Our expertise:
• We assist research teams with the development of robust bioassays compatible with
HTS/HCS standard from the assay optimization to the miniaturization and automation.
We also provide guidance for the design of orthogonal (secondary selectivity) assays.
• We conduct the screening campaigns of libraries of interest, upload and analyze the data, using our carefully annotated database (Collaborative Drug Discovery), cherry-pick hits for re-testing in dose response experiment (EC50 determination) in order to provide you with a high-quality hit list.
• We provide strong support for hit to lead and lead optimization processes (medicinal
chemistry, ADME/Tox studies, MOA studies, aggregation/solubility test), especially
for some promising projects with high therapeutic index.

Technologies we support:
• Biophysical techniques using absorbance, fluorescence, luminescence such as timeresolved fluorescence resonance  energy transfer (TR-FRET), AlphaScreen, fluorescence polarization allow for the miniaturization of assays which measure enzyme, receptors, protein:protein/DNA/RNA interactions. Biolayer interferometry technology enables for high-throughput label-free detection using the Octet HTX instrument (ForteBio, Molecular Devices).

• Automated microscopy and reporter gene assays allow for measurement of phenotypic changes and signal transduction processes. For HCS, phenotypic analysis in single cell (2D) or organoids (3D) can be carried out with a conventional automated microscope (IX83, Olympus) or the high-resolution microscope StellarVision (Optical Biosystems) that uses structured illumination and Synthetic Aperture Optics (SAO).

• With our liquid acoustic dispenser (Echo550, Labcyte), we can easily reformate multiwell plates (96, 384, 1536 wells), offer cherry picking service and prepare any daughter plates from various biological and chemical collections.

How to work with us:
A request for support starts by sending an email to pfccb@pasteur.fr. You will be asked to give us an overview of your project at first and then meet in person to give us any details so we can figure out together the best way to develop your HTS or HCS bioassay. We can assist you to become autonomous on some automated equipment, help you during every steps of the screening process and can perform for you a medium and large screening campaign.

 

Libraries :


Fundings:
   

Equipments

Booking site
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Array

Tycho • Nanotemper • NT.6

Tycho verifies protein quality by looking at the structural integrity (or foldedness) of a protein. As a thermal ramp is applied, the changes in the intrinsic fluorescence, detected at both 350 nm and 330 […]

Grating Coupled Interferometry • Malvern • Creoptix WAVEcore

The Creoptix® WAVEsystem combines crude sample robustness, high sensitivity, and sophisticated software, to create a novel optical biosensor platform for real-time label-free binding kinetics. Engineered around innovative microfluidics and the Grating-Coupled Interferometry (GCI) technology, […]

qPCR instrument • Applied Biosystems • Quantstudio 6 Pro

The Applied Biosystems QuantStudio 6 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint. With facial authentication, voice commands, Smart Help, multiplex capability, motorized […]

qPCR instrument • BioRad • CFX96

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to […]

Viaflo • Integra Biosciences • Viaflo 96 & 384

VIAFLO 96 and VIAFLO 384 enable transfers of 24, 96 and 384 samples in a single step, thus increasing pipetting  throughput and reproducibility significantly. The hands-free automatic mode enables the instrument to move and […]

Multimodal Plate Reader • Tecan • Spark

The Spark multimode microplate reader offers solutions for virtually any life science research or drug discovery  applications. The unique Fusion Optics allows any combination of filters or monochromators on both the excitation AND emission […]

Plate washer and dispenser • Biotek • EL406

The BioTek EL406 washer dispenser is a compact instrument offering fast, full plate washing along with three reagent dispensers. The EL406 capabilities include washing and dispensing for standard assays, plus biomagnetic separation and vacuum […]

Echo Liquid Handler System • Labcyte • Echo 550

The Echo 550 instrument enables non-contact liquid handling using sound energy, high speed, contamination-free transfer of cDNA, siRNA vectors, proteins and crystallography reagents – in nanoliter increments. Echo Plate Reformat software offers a variety […]

Octet Instrument • ForteBio • Octet HTX System

The Octet HTX instrument monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed. The system’s ability to read 8, 16, 32, 48 or 96 wells […]

Microscope • Olympus • IX83

The fully motorized IX83 is the most advanced member of the IX3 series of inverted imaging systems and offers a new level of application flexibility. Choose from the one-deck system with a low, ergonomic […]

Microscope • Optical Biosystems • StellarVision

Target-based, cell-based and phenotypic/microscopy screening assay have numerous advantages and disadvantages and an important challenge in the near future will be to effectively incorporate new screening technologies into phenotypic screening approaches since the traditional […]

Plate Reader • Wyatt Technology • DynaPro Plate Reader II

For small molecules, the DynaPro Plate Reader II can easily detect compound aggregates and aggregation inducers which often result from High-Throughput Screening (HTS) campaign. These primary Hits are often considered as false-positives with poor […]

Members

Former Members

(FR)

Name Year Position Linkedin
Sharona Raveh 1997-2001 PhD student linkedin.com/in/sharona-raveh-a3130020
Emilie Vinolo 2003-2006 PhD student https://www.linkedin.com/in/evinoloprofile/
Emmanuel Wyler 2004-2005 Master student linkedin.com/in/emanuel-wyler-6130b034
Samuel Levy 2011 PhD student (MD) linkedin.com/in/samuel-levy-543b001
Emmanuel Laplantine 2010-2011 Post-Doc linkedin.com/in/emmanuel-laplantine-92789a1b0
Ricardo Henriques 2008-2011 PhD student linkedin.com/in/ricardo-henriques-lab
Virginie Dubosclard 2010-2013 Post-Doc linkedin.com/in/virginie-dubosclard-b3020781
Flora Ngadjeua Tchouatieu 2009-2013 PhD student linkedin.com/in/flora-ngadjeua-phd-5a480852
Lauriane Davignon 2016 Post Doc linkedin.com/in/lauriannedavignon
Marine Charpentier 2017 Post Doc linkedin.com/in/marine-charpentier-671b575b
Afolake Arowolo 2018 Research officer linkedin.com/in/afolake-arowolo-11a22627
Keis Nabhane Said Halidi 2014-2017 PhD student linkedin.com/in/keïs-nabhane-said-halidi-phd-36a590180
Sara Consalvi 2017-2018 Post Doc linkedin.com/in/sara-consalvi-99322473
Olivier Borkowski 2018-2019 Post-Doc linkedin.com/in/olivier-borkowski-025b8a49

Transversal Project

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Contact

Email: pfccb@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France