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© Biologie structurale et chimie
Structure du domaine en doigt de zinc de la protéine NEMO, déterminée par Résonance magnétique nucléaire (RMN). Cette protéine jouant un rôle dans des maladies (cancer, inflammation), les connaissances acquises sur sa structure offrent de précieuses informations sur sa fonction.

About

Chemogenomic and biological screening are important steps in precision medicine as well as in the discovery of new medicines but also in dissecting molecular pathways and biological networks.

PF-CCB supports researchers in improving the efficiency of their target-based (HTS) and phenotypic (HCS) screening bioassays, in identifying active substances and genetic modulators of function (siRNA, miRNA, gRNA) and in using core technologies typically applied to miniaturization and automation of bioassays.

We also support drug repositioning via an unique updated FDA-approved library and develop innovative methods (i) to screen for agents that alter a subtle change of phenotype in a single cell (2D) or organ (3D), (ii) to isolate protein-protein interaction inhibitors using label free technology, (iii) to carry out reverse and normal transfection of genetic modulators using acoustic liquid dispensing technology, and (iv) to identify protein targets of your hits (target deconvolution).

What can we do for you ?

We assist research teams with the development of robust target-based (HTS) and phenotypic (HCS) assays, and provide high quality Hits (small molecules, peptides and natural compounds). For HCS, phenotypic analysis can be carried out either with a conventional automated microscope (IX83, Olympus) or the new microscope Stellarvision that uses structured illumination and Synthetic Aperture Optics (SAO). For HTS screening assay technologies, we have the ability to support cellular and biochemical assays using absorbance, fluorescence anisotropy, time-resolved fluorescence, time-resolved fluorescence resonance energy transfer, AlphaScreen and luminescence. We make also available the Octet HTX instrument, which enables high throughput label-free detection via Biolayer interferometry technology (fortebio, Pall life sciences). With our liquid acoustic dispenser (Echo550, labcyte), we can very easily reformate multiwell plates for you (384 and 1536 wells), offer cherry-picking service and prepare any daughter plates from various biological and chemical collections. Finally, we also provide strong supports for developing drug candidates (hit to lead and lead optimization), especially for some promising projects with a high therapeutic index.

Our expertise includes:

  • Microscopy/phenotypic-based screening assay with high resolution imaging (HCS)
  • Target-based screening assay (HTS)
  • Protein-protein and RNA-protein interaction inhibitors
  • Medicinal chemistry
  • Target deconvolution
  • Various functional and chemical libraries including small molecules, peptide, antibody-like protein and siRNA (ubiquitome)

How can you apply for support ?

A request for support starts by sending an email to pfccb@pasteur.fr. You will be asked to give us an overview of your project at first and then meet in person to give us any details so we can figure out together the best way to develop your HTS or HCS bioassay. We can assist you to become autonomous on some automated equipment, help you during every steps of the screening process and can perform for you a medium and large screening campaign.

Events

Scientific Day on Genome Editing Technology : CRISPR-Cas9 Seminar

Building: Monod (66) – Room: Amphithéâtre – Address: Institut Pasteur, Paris, France
2016-02-11 10:00:00 2016-02-11 00:00:00 Europe/Paris Scientific Day on Genome Editing Technology : CRISPR-Cas9 Seminar 10:00 – 11:00 : Fundamental and innovations in CRISPR-Cas9 gene editing James Goldmeyer, PhD, Product Manager GE Healthcare Dharmacon 11:00 – 11:30 : iCRISPR: inducible editing in stem cells and their derivatives Janine Scholefield, […] Institut Pasteur, Paris, France

Advancing scientific innovations with ECHO Acoustic Liquid Handling

Building: Lwoff – Room: RDC-14/15 – Address: Institut Pasteur, Paris, France
2016-04-15 09:30:00 2016-04-15 16:00:00 Europe/Paris Advancing scientific innovations with ECHO Acoustic Liquid Handling 9,45 – 10,30 : Acoustic Dispensing Usage for Drug Discovery in the CRUK Cancer Therapetics Unit. Dr Rosemary Burke, Senior Researcher Hit Discovery and Structural Design Team, CRUK London 10,45 – 11,30 : Single […] Institut Pasteur, Paris, France

The power of CRISPR-Cas9 arrayed screen using synthetic crRNA libraries

Building: Monod (66) – Room: Amphithéâtre – Address: Institut Pasteur, Paris, France
2017-03-15 10:00:00 2017-03-15 00:00:00 Europe/Paris The power of CRISPR-Cas9 arrayed screen using synthetic crRNA libraries CRISPR-Cas9 is a powerful tool for loss-of-function analysis, and to date has been adopted for large-scale functional screening primarily in the form of pooled sgRNA libraries. However, pooled screening is limited to enrichment or […] Institut Pasteur, Paris, France

Transversal Project

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Phone: +33 (0)1 40 61 38 10
Email: pfccb@pasteur.fr

Address
25-28 Rue du Docteur Roux
75015, Paris
France