Photonic BioImaging is a Unit of Technology and Service (UTechS) providing optical imaging expertise in life sciences and especially their application in studies on infectious biology.

Our activities include service rendering, training, technology-driven research and technology development. They are highly multi-disciplined, and collaborative, with the mission goal focused on the use of quantitative imaging and analysis to understand the processes of cell/tissue-biology, and their usurpation by infection and disease. The R&D is founded upon the need to develop optical imaging methods that bring new understanding of host-pathogen interactions and in situ high-content imaging techniques and their application to infection, cell biology, cellular microbiology, and microbiology. We work on novel techniques extrapolating quantitative information on spatiotemporal dynamics in situ and we push the limits of existing approaches aiming to enhance their performance thereby broadening their experimental utility.

Practical Information

Instrument booking, training/project request :

PPMS Booking
(intranet access only)

Open Desk :

The Photonic BioImaging team (Imagopole) offers a bi-weekly open-desk.The goal of the open-desk is to make the PBI engineers available for informal discussions about your forthcoming projects, or any imaging-related question you might have. There is no need to schedule an appointment, just come with your questions. Open-desks are planned to happen every two weeks, same place same time (2:00pm to 3:30 pm , in the Centre François Jacob atrium).

Planned Opendesk/initial training schedule

April 15^th Initial Theorie Training
April 22^nd OpenDesk cancelled (Holliday)
April 29th Initial Theorie Training
May 6^th OpenDesk
May 13^th Initial Theorie Training (to be confirmed)
May 20^th OpenDesk
May 27^thInitial Theorie Training
June 3^rd OpenDesk
June 10^th Initial Theorie Training cancelled (Holliday)
June 17th OpenDesk
June 24^th Initial Theorie Training
July 1^st OpenDesk

pbi.contact@pasteur.fr

Feedback / Comments

pbi.feedback@pasteur.fr

Satisfaction surveys.

PBI runs a satisfaction survey at least every 2 years, addressed to its past 2 years users. The following files link to the analysis of the surveys.

Equipments

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Microscope spinning-disk Ti2E • Nikon • Ti2E - Yokagawa CSU W1

Spinning-disk Ti2E Inverted microscope, equipped with a Yokagawa CSU-W1 spinning disk with 2 pinhole size, and two cameras: a more sensitive and another more resolutive. It allows the fast acquisition of optically sectioned slices at […]

Light Sheet microscope – Lavision Biotech • Lavision Biotech • UltraMicroscope II

imaging in aqueous buffers and organic solvent optical zoom from 1.26x to 12.6x large sample uptake up to 30 mm x 30 mm x 15 mm dynamic horizontal focusing for better Z resolution 6 […]

FMT 4000 Perkin Elmer • Perkin Elmer • FMT 4000

Small animal pre-clinical in vivo imaging system dedicated to 3D imaging of fluorescence signal (Fluorescence Molecular Tomography). The system is equipped with 4 lasers in the Near Infra Red and is calibrated for quantifying […]

IVIS Spectrum CT Perkin Elmer • Perkin Elmer • IVIS Spectrum CT

Small animal pre-clinical in vivo imaging systems dedicated to 2D and 3D imaging of bioluminescence and fluorescence signals. The system is equipped with 10 excitation filters and 18 emission filters, and a high sensitive […]

IVIS Spectrum Perkin Elmer • Perkin Elmer • IVIS Spectrum

Small animal pre-clinical in vivo imaging systems dedicated to 2D and 3D imaging of bioluminescence and fluorescence signals. Each system is equipped with 10 excitation filters and 18 emission filters, and a high sensitive […]

CellVoyager CV1000 • Yokagawa • CV1000

Presentation. The Yokagawa CellVoyager CV1000 is an automated microscope based on the spinning-disk technology. It excels at multi-scale microscopy, when locating rare events in a large sample is needed. Applications typically consist in […]

BioAxial CODIM100 • BioAxial • CODIM100

Presentation. The CODIM 100 is a super-resolution microscope that specialized in imaging live-cell. It is the fruit of a collaboration between the Imagopole and the BioAxial company, on the development of an entirely new approach […]

Microscope confocal SP5 HyD inverted • Leica • SP5 HyD resonnant scanner Matrix screener

Inverted microscope fully motorized, equipped with a laser scanning confocal head. It allows the acquisition in spectral mode, multi-positions, multi-mosaics, and autofocus control with sensitive detectors. Features : Available light sources 405 nm […]

Microscope confocal SP8 inverted • Leica • SP8

Inverted microscope fully motorized, equipped with a laser scanning confocal head. It allows the acquisition in spectral mode, multi-positions, multi-mosaics. Features : Available light sources 405 nm White laser 470-670 nm Mercury […]

Microscope spinning-disk UltraView VOX • Perkin-Elmer • UltraView VOX

Spinning-disk UltraView VOX Inverted microscope, equipped with a Yokagawa CSUX1 spinning- disk, and two cameras to do dual-color simultaneous imaging. It allows the fast acquisition of optically sectioned slices at very high speed, and is […]

Microscope confocal LSM780 ELYRA PS.1 • ZEISS • LSM 780 - Elyra PS.1

Inverted confocal microscope (780 head) coupled with two techniques of super resolution SIM and PALM STORM (Elyra PS.1 head) SR SIM : Super resolution structured illumination PALM/STORM : Photoactivated Localization Microscopy / Stochastic Optical […]