Julien Fernandes

EDUCATION 2006-2010, Doctorate of Philosophy degree in cell biology. Cergy-Pontoise University. 2005-2006,  Master Degree (2^nd year) in Biochemistry and Biophysics. Cergy-Pontoise University. 2004-2005, Master Degree (1^st year) in Molecular and Cellular Biochemistry. Cergy-Pontoise University. 2000-2004, Bachelor of Science in Biochemistry. Cergy-Pontoise University.   WORK EXPERIENCE March 2016, Research Engineer – Centre d’Innovation et Recherche Technologique – Photonic BioImaging , PASTEUR Institute

• Practical and theorical training on multi-photon, Confocal and epifluorescence microscopy
• Project research
• Maintenance and metrology

April 2012- Feb 2016, Post-doctoral researcher – Physiopathology and epidemiology of respiratory diseases lab, Paris Diderot University and Aspergillus Lab, Institut PASTEUR. « Interactions between Aspergillus fumigatus and respiratory epithelial cells ». Design and study of a new model of infection in the context of aspergillosis. Advisor: Dr Marina Pretolani and Pr Jean-Paul Latgé. Funding: AVIESAN and Leg Poix  Sept 2010- March 2012, Post-doctoral researcher – Pathogenesis of Helicobacter Unit – PASTEUR institute. «Study of mitochondrial alteration during infection by Helicobacter pylori and uses as a biomarker for gastric cancerogenesis ». Design of a new biomarker for studying the gastric cancerogenesis leading to the deposit of an International patent (WO 2015/049372 A1). Advisor: Dr Eliette Touati. Funding: Fondation Odyssey Re. March 2007 – Aug 2010, Ph.D. thesis – ERRMECe lab – Cergy-Pontoise University. « Study the multicellular aggregates formation of ovarian carcinomas and remodeling of the tumor microenvironment». Advisor: Pr Franck Carreiras. Funding: Ligue contre le Cancer. Oct 2006-March 2007, Feasibility associate – PAREXEL International, Paris.

• Creating feasibility survey before clinical trials
• Collecting all the data from international collaborators
• Writing reports for the clients

TECHNICAL SKILLS Microscopy Epifluorescence and confocal microscopy 2 photon microscopy Time-lapse microscopy Transmission and scanning electron microscopy Focused ion beam microscopy Cellular Biology Cell lines and primary cells Suspension cell culture Air-liquid interface cell culture Molecular Biology RNA and DNA extraction RNA and DNA quantification Quantitative real-time PCR, Reverse Transcriptase PCR Primer design Microbiology, Infectiology Fungi (Aspergillus fumigatus), bacteria (Helicobacter pylori) Biochemistry Immunocytochemistry Immunohistochemistry Western-Blot, Zymography Multiplex, ELISA Animal experimentation Genotyping TEACHING AND SUPERVISING EXPERIENCE 2009 – 2011, Teaching assistant – Biology department – Cergy-Pontoise University. Cell Biology basis, organization of cells into tissues, cell culture, host-pathogen interaction. 2008-2014, Internship supervisor

• Physiopathology and epidemiology of respiratory diseases lab: 1 PhD and 1 Master students
• ERRMECe lab: 3 Master students

LINGUISTIC AND COMPUTER SKILLS

• French, English (Fluent)
• Microsoft Office (Word, Excel, and Power Point)
• Microscopy software: ZEN, LAS, Bitplane (Imaris), ImageJ, Metamorph, Huygens Software
• Data analysis: Graphpad

SCIENTIFIC COMMUNICATION  Oral communication in international symposium

1. « Interaction between fumigatus and the respiratory epithelium », 2014, Filamentous fungal pathogens: current trends and future perspectives, Madurai, India.

 Publications in international journals

1.  Fernandes, V. Michel, M. Camorlinga‐Ponce, A. Gomez, C. Maldonado, H. De Reuse, J. Torres and E. Touati, 2014, « Circulating mitochondrial DNA level, a non‐invasive biomarker for the early detection of gastric cancer », Cancer Epidemiology, Biomarkers and Prevention.
2. Fernandes & S. Kellouche, J. Leroy-Dudal, O. Gallet, S. Dutoit, L. Poulain, F. Carreiras, 2010, « Initial formation of IGROV1 ovarian cancer multicellular aggregates involves vitronectin», Tumor Biology.
3. Heyman, J. Leroy-Dudal, J. Fernandes, D. Seyer, S. Dutoit, F. Carreiras, 2010, «Mesothelialvitronectin stimulates migration of ovarian cancer cells », Cell Biology International.
4. Heyman, S. Kellouche, J. Fernandes, S. Dutoit, L. Poulain and F. Carreiras. 2008 «Vitronectin and its receptors partly mediate adhesion of ovarian cancer cells to peritoneal mesothelium in vitro », Tumor Biology.

 Patent

1. Touati, J. Fernandes, V. Michel and J. Torres Lopez, « Method for in vitro investigating mitochondrial DNA levels in a biological sample kits and uses thereof ». International publication number : WO 2015/049372 A1

Published acts of conferences

1. C. Alvarez, J. Fernandes, E. Touati and M. Lima Ribeiro, 2011, «Modulation of GATA 5 by Helicobacter pylori infection, in vitro and in vivo», XXIVth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation, Dublin, Irlande.
2. Fernandes, S. Kellouche, L. Heyman, L. Carduner, O. Gallet, J. Leroy‐Dudal and F. Carreiras, 2009, «Involvement of microenvironment vitronectin and fibronectin in human ovarian cancer cell dissemination: cell aggregates formation and extracellular matrix remodeling», 5^èmeconference Internationale sur le microenvironement tumoral: Progrès, Thérapie et Prévention, Versailles, France.
3. Fernandes, L. Heyman, J. Leroy‐Dudal, L. Poulain, R. Agniel et F. Carreiras, 2006, «Invasion de MEC endothéliale et mésothéliale par les cellules d’adénocarcinomes ovariens humains: cas particulier du remodelage de la fibronectine », XXVème Forum de cancérologie, EUROCANCER, Paris, France.

 16 posters presentation in international and national congresses