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© Research
Equipment

CellVoyager CV1000

Scientific Fields
Diseases
Organisms
Applications
Technique

Yokagawa • CV1000

Presentation.

The Yokagawa CellVoyager CV1000 is an automated microscope based on the spinning-disk technology. 

9315f7847e

 

It excels at multi-scale microscopy, when locating rare events in a large sample is needed. Applications typically consist in scanning the sample at low magnification to locate events, then switching to a high magnification objective to acquire high-resolution, 3D images of the event. 

The microscope is not limited to fixed samples, as it ships a full environmental control to do live cell experiments. 

It is a spinning-disk confocal microscope, and hereby offers the ability to acquire quickly and with a high sensitivity 3D optically-sectioned slices of the sample. 

Specifications.

  • Objectives installed: 10x air (0.4 NA); 20x air (0.7 NA), 40x air (0.95 NA), 40x oil (1.3 NA), 60x water (1.2 NA) and 60x oil (1.4 NA).
  • Three laser lines: 405nm, 488nm, 561nm.
  • Emission filters:
    • BP1: 417 – 477 nm (DAPI and co.)
    • BP2: 500-525 nm (AF488 and co.)
    • BP3: 600-633 nm (AF568 and co.)
  • Transmitted light.
  • High-sensitivity camera (EM-CCD, 92% nominal QE).
  • Autofocus mechanism, both in software mode (classical) and in hardware mode (finds the surface of the coverslips by itself).
  • Full environmental control: CO2 and temperature. 
  • High precision XY stage to build large areas of imaging thanks to tiling.
  • Intuitive software.
  • Carriers for microscopy slide, 3.5cm ø glass-bottom petri dishes and microplates (92 well plates and 384 well plates).

Resources.

Example images:

MAX_3_3_8_2_1-1

1_7_6_1_5-1

(blue: DAPI; green: Shigella Flexneri; red: actin-phalloidin)

Taken from: 

Bioimage analysis of Shigella infection reveals targeting of colonic crypts ET Arena, FX Campbell-Valois, JY Tinevez, G Nigro, M Sachse, M. Moya-Nilges, K Nothelfer, B Marteyn, SL Shorte, P Sansonetti – PNAS, 2015