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  • Undergraduate Student
  • Veterinary
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  • Deputy Director of Center
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  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
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© Research
Equipment

Microscope Super resolution SIM/SMLM Elyra 7

Scientific Fields
Diseases
Organisms
Applications
Technique

ZEISS • Elyra 7

This particular system is equipped with two of the major super resolution modalities Lattice SIM (Structured Illumination Microscopy) and 3D SMLM (Single Molecule Localisation Microscopy) allowing the observation of fixed and living biological phenomena at spatial and temporal resolution scales not accessible with conventional microscopes. ( Achievement of a lateral precision of localisation of 10-20nm  using SMLM, and using the other modality Lattice SIM a temporal resolution up to 19 images/sec). The microscope is also equipped with 2 cameras, an incubator, a CO2 controller and a drift correction system offering ideal conditions for live imaging.

Single Molecule Localisation Microscopy Left image : U2OS cell line labelled with Nup96 to visualise the nucleopore structure. The image acquired and processed in 3D SMLM have been color coded in depth and you can see in the inset the ultrastructure of a nucleopore with a size of 10-20 nm for each of the 8 nucleoporins 96. Right image : Hela cells were labelled with phalloidin to visualise actin meshwork. The image have been acquired and processed in 2D SMLM TIRF.

 

Features :

 Available laser lines for excitation

  • 405 nm – 50 mW
  • 488 nm – 500 mW
  • 561 nm – 500mW
  • 642 nm – 500 mW
  • Fluorescent Lamp HXP 120

Objectives

  • 10X/0.3 : EC Plan Neofluar
  • 20X/0.8 : Plan Apochromat
  • 40X/1.4 : Plan Apochromat
  • 63X/1.4 : Plan Apochromat
  • 63X/1.46 : Plan Apochromat

Filters for observation and acquisition  :

  • Observation : Multiband Filter 25 HE
  • Acquisition :
    • Quad band filter
    • BP 420-480
    • BP 495-590
    • LP 570

Detectors-

  • 2 cameras PCO Edge 4.2 QE 82%
  • XY Piezo scanning stage
  • z-piezo stage
  • Focus control by Definite focus 2
  • temperature and CO2 control
  • Stand: Zeiss Axio observer 7 with touch screen
  • Acquisition software : ZEN

 

Capabilities:

  •  Lattice SIM
  • Apotome
  • dual Camera
  • TIRF
  • SMLM 3D
The Super Resolution microscope, “Elyra 7” from Carl Zeiss Gmbh (Jena, Germany) have been partially financed by Institut Pasteur, Labex IBEID and DIM1HEALTH