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© Research
Equipment

Microscope confocal LSM780 ELYRA PS.1

Scientific Fields
Diseases
Organisms
Applications
Technique

ZEISS • LSM 780 - Elyra PS.1

ELYRA

Inverted confocal microscope (780 head) coupled with two techniques of super resolution SIM and PALM STORM (Elyra PS.1 head)

  • SR SIM : Super resolution structured illumination
  • PALM/STORM : Photoactivated Localization Microscopy / Stochastic Optical Reconstruction Microscopy

It contains also a highly sensitive spectral detector GaAsp of 32 channels.

Features :

 Available laser lines for excitation

  • Confocal
    • 405 nm (30 mW) – Solid
    • 458, 488, 512 nm (25 mW) -Argon laser
    • 561 nm (20 mW) – Solid
    • 594 nm (2 mW) – HeNe
    • 633 nm (5 mW) – HeNe
  • ELYRA
    • 405 nm (50 mW) HR (High Resolution) – Solid
    • 488 nm (100 mW) HR -Solid
    • 561 nm (100 mW) HR -Solid
    • 642 nm (100 mW) HR -Solid
  • Halogen lamp X-cite

 

Objectives

  • 10X : EC PlnN 10X/0.3 DIC I
  • 40X W : C Apo 40X/1.2 W DIC III
  • 63X oil : Pln Apo 63X/1.4 oil DIC III
  • 100X oil : alpha Pln Apo 100X/1.46
  • 100X oil : alpha Pln Apo 100X/1.57

 

Filters for observation and acquisition :

  • Observation
    • One triple band pass filter
  • Acquisition
    • BP 420-480 + LP 750
    • BP 495-575 + LP 750
    • BP 570-650 + LP 750
    • LP 655

 

Detectors and cameras

  • 2 Photomultipliers (PMT)
  • Photomultiplier for transmission (T-PMT)
  • Spectral 32 channels detector GaAsp
  • EMCCD Andor Ixon 897 0.5K : PALM/STORM
  • EMCCD Andor Ixon 887 1K : SIM

 

  • motorized XY stage MCU 2008
  • z-piezo PI
  • Environmental chamber with temperature control
  • Focus control by Definite focus
  • Stand: Zeiss Axio observer Z1 HR  with touch screen
  • Acquisition software : ZEN

 

Capabilities:

  •  Confocal imaging
  • Spectral confocal imaging
  • Widefield
  • SR SIM
  • PALM/STORM
  • TIRF
  • FCS
  • RICS
  • FRAP
  • Multi point and tiled images

 Sample preparation

SIM

20140213_protocole_SIM

 Guide for SIM acquisition and process

Elyra-SIM-Guide-2013-V2

More information

SIM