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  • program_project
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  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
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  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
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© Research
Equipment

Microscope widefield Definite Focus

Scientific Fields
Diseases
Organisms
Applications
Technique

ZEISS • Definite focus

Definite_focus

Inverted widefield Microscope equipped with LED colibris  and mercury lamp allowing acquisition on live fluorescent samples with control of the focus using the ‘Definite Focus’ module.

 

Features :

Available light sources

  • Mercury Lamp HXP120
  • LED colibri
    • 365 nm
    • 470 nm
    • 555 nm
    • 590 nm

Available filters

Carousel # Example fluorophores Excitation Dichroic Emission Commercial name
#1 DAPI, Hoechst, Alexa Fluor 405, DyLight 405 BP 359-371 FT 395 BP 421-479 Zeiss FS01, modified emission
#2 eGFP BP 450-490 FT 495 BP 500-550 Zeiss FS38 HE
#3 Cy3, TRITC, Alexa Fluor 546, Alexa Fluor 555, DsRed BP 540-552 FT 550 BP 565-595 Omega XF37
#4 Cy5, Alex Fluor 633, Alex Fluor 647, DRAQ5 BP 605-645 FT 650 BP 668-723 Omega XF110-2
#5 Interferential Contrast       DIC
#6 HcRed, TexasRed BP 576-596  FT 605 BP 668-723  Semrock HcRed

Objectives

  • 10X : PlnN 10X/0.3 Ph 1
  • 20X : LD Achroplan 20X/0.4 Ph 2
  • 25X : PlnN  25X/0.8  Oil-W-GlycPh 2
  • 40X : Pln-Apo 40X/1.3 oil Ph3
  • 63X : Pln-Apo 63X/1.4 oil Ph3
  • 100X : PlnN 100X/1.4 oil

Detectors

  • Camera Axiocam MRm
  • Zeiss Definite Focus as hardware focus stabilization.
  • motorized XY stage MAC 5000
  • Stand: Zeiss Axio Observer Z1 with touch screen
  • Environmental chamber with temperature, CO2 and O2 control
  • Acquisition software : ZEN blue 2012

Capabilities:

  • Multi Channel
  • Measurement
  • Z-Stack
  • Time series
  • Tiles
  • Panorama
  • Experiment Designer
  • Software Autofocus