I’m a trained biophysicist and develop integrated approaches combining fluorescence imaging, computerized image analysis, and mathematical modeling. Equipped with these skills, I want to contribute to a better quantitative understanding of biological processes. My current focus is on gene expression, however, this integrated approach can be readily applied to other biological questions where imaging and modeling form an integral part to obtain a deeper quantitative understanding.
Fluorescence microscopy is a defining technology in cellular biology but the diffraction of light limits the resolution of standard microscopes to ~200 nm, thus preventing detailed analyses of molecular structures. We are actively interested […]
Nuclear architecture and function
The one-dimensional sequence of the genome is carried by long polymers (chromosomes), which fold in the three dimensional volume of the nucleus. How this folding occurs and what it implies for genome function remains […]
FISH-quant: automatically counting transcripts in RNA-FISH images
A free Matlab-based toolbox to detect and count individual mRNA transcripts in single cells by automatic analysis of 3D RNA-FISH images. Software is available freely together with extensive instructions at : https://code.google.com/p/fish-quant/. The […]
- Graz University of Technology, Austria and National Institutes of Health (NIH), Bethesda; 2005 – 2008
- Thesis: Quantitative analysis of the dynamic properties of chromatin associated proteins by FRAP experiments
- Advisors: Dr. James G. McNally, Dr. Zlatko Trajanoski
- Computational Physics, Universität Graz, Austria, 1999 – 2005
- Thesis: Numerical Simulations of FRAP Experiments
- Advisors: Dr. Max Lippitsch, Dr. Zlatko Trajanoski
2020A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting., Dev. Cell 2020 Aug; (): .
2020mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage., Development 2020 Jun; (): .
2020Remodeling of the Core Leads HIV-1 Preintegration Complex into the Nucleus of Human Lymphocytes., J. Virol. 2020 May;94(11):e00135-20.
2019ImJoy: an open-source computational platform for the deep learning era, Nat. Methods 2019 Dec;16(12):1199-1200.
2019A Deep Learning Approach To Identify MRNA Localization Patterns, IEEE 16th International Symposium on Biomedical Imaging, 2019; 1386 - 1390.
2019Co-translational assembly of mammalian nuclear multisubunit complexes, Nat Commun 2019 04;10(1):1740.
2019The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells, Nat Commun 2019 03;10(1):1109.
2018A computational framework to study sub-cellular RNA localization, Nat Commun 2018 Nov;9(1):4584.
2018A Growing Toolbox to Image Gene Expression in Single Cells: Sensitive Approaches for Demanding Challenges, Mol. Cell 2018 Aug;71(3):468-480.
2018Visualization of Arenavirus RNA Species in Individual Cells by Single-Molecule Fluorescence Hybridization Suggests a Model of Cyclical Infection and Clearance during Persistence, J. Virol. 2018 06;92(12).
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