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© Research
Equipment

Microscope spinning disk BC43 – Benchtop confocal 43

Scientific Fields
Diseases
Organisms
Applications
Technique

Andor • BC43

This system is an automated microscope equipped with two fluorescent imaging modalities: widefield and confocal (Borealis™-illuminated multipoint). This system is easy to use, offers different support, suitable for a wide variety of sample types in fixed or live condition (temperature, humidity, and oxygen control), from subcellular and cellular experiments to large tissue samples and organoids requiring deeper imaging.

Image from Institut Pasteur, François Gardy

Features :

 Available excitation wavelengths

  • Transmitted light (LED): brightfield and DPC
  • Widefield (lasers – centre λ/bandwidth):
    • 405 (445/20 nm)
    • 488 (529/24 nm)
    • 561 (595/31 nm)
    • 638 (708/75 nm)
  • Confocal (lasers – centre λ/bandwidth) :
    • 405 (445/20 nm)
    • 488 (529/24 nm)
    • 561 (595/31 nm)
    • 638 (708/75 nm)

Objectives

  • 2x/0.06  : Plan UW, working distance 7,5 mm
  • 10x/0.45: Plan Apochromat, WD 5 mm
  • 20x/0.8 : Plan Apochromat, WD 0,8 mm
  • 40x/0.95: Plan Apochromat, WD 0,25 mm
  • 60x/1.42 : oil immersion Plan Apochromat, WD 0,15 mm, with objective heater

Detectors

  • sCMOS 4.1 Mpixels, 6.5µm pixel, 16-bit, QE 82%
  • Incubation chamber
  • Temperature, humidity and CO2 control
  • Acquisition software : Fusion Benchtop 1.1.0.1
  • Image analysis software with AI tools: Imaris 10.0.1

Capabilities:

  • Widefield
  • Confocal 
  • 3D Deconvolution
  • Focus stabilisation

More informations

https://andor.oxinst.com/products/bc43-benchtop-confocal-microscope