Mariette Matondo

Intracellular bacterial pathogens enter host cells to form a bacterial containing vacuole (BCV). Then, the BCV matures or ruptures leading to two distinct pathogen-dependent intracellular niches; one vacuolar and one cytosolic. The specific intracellular […]

A common phenomenon in most microbial infections is the attenuation of the host’s immediate immune response, allowing for long-term colonization. We and others have shown that many pathogens achieve this by manipulating the epigenetic […]

Free-living bacteria commonly cope with and adapt to changing environments. We have shown that various families of antibiotics are capable, at concentrations as low as 1% of the MIC, to activate stress responses in […]

This project is a part of the international Infect-Era program, AMOEBAC: Entamoeba histolytica-bacterium interaction and the role of this interaction in intestinal pathogenesis (http://www.infect-era.eu/2nd-call-2014) The aim of this investigation is to understand the induced […]

The European Proteomics Infrastructure Consortium providing access (EPIC-XS) consists of a unified network of experienced access providers and research groups who share a common goal; to facilitate the development and sustainability of proteomics exploration […]

CNF1 is a toxin that catalyzes the permanent activation of host RhoA, Rac1 and Cdc42 small GTPases by selective deamidation of a glutamine residue into a glutamic acid. This 1 dalton post-translational modification can […]

Cilia and flagella are essential eukaryotic organelles composed of an evolutionary conserved structure of 9 doublet microtubules that are similar but not equivalent. We propose that a tubulin post-translational modification called glutamylation could act […]

Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]

Our PTR project investigates at the complex interface between the intracellular pathogen Leishmania and its macrophage host cell. We will apply systems-wide analyses to elucidate the molecular mechanisms underlying the reciprocal metabolic relationship between […]

Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most […]

Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a […]

Within cells, proteins are the real effectors of all activities. The expression of certain proteins changes upon cell stimulation or stress (with virus, inhibitors or bacteria), or when cells differentiate or turn into a […]

ProteoCombiner is a high-performance software integrating shotgun with top-down proteomic data. The tool can deal with multiple experiments and search engines. It enables rapid and easy visualization, manual validation, and comparison of the identified […]

Successful extension of mass spectrometry-based (MS) proteomics to single cells is very challenging, mainly due to low analysis throughput and insufficient proteome coverage in single cells. To address these challenges, new pipelines have emerged […]