I have been the manager of the Pasteur Proteomics facility since September 2013. I am responsible for the operations of the proteomics platform and the coordination of the different ongoing projects. I did my PhD research in the group of Dr. Bernard Monsarrat and Dr. Odile Schiltz at the Institute of Pharmacology and Structural Biology (IPBS, University Paul Sabatier, UPS, also known as Toulouse III, France). As part of my PhD work, I used high-resolution mass spectrometers, including the LTQ-Orbitrap, in combination with various quantitative proteomic approaches. My focus was on stable isotope labelling strategies (SILAC and ICAT) to study the proteasome inhibitor effect in acute myeloid leukaemia cells. I then moved to the Institute of Molecular Systems Biology in Eidgenössische Technische Hochschule (ETH Zürich) as a post-doctoral fellow in the group of Professor Ruedi Aebersold. I worked on multiple projects using mass spectrometry to assess changes in protein abundance and posttranslational modifications, e.g., ubiquitin, SUMO, and chlorination of proteins using discovery-driven and targeted mass spectrometry. During this time, I utilised the new data independent acquisition mode developed in Ruedi Aebersold Lab, namely SWATH-MS.
Role of tubulin glutamylation in flagellum construction (TUBGLUFLA)
Cilia and flagella are essential eukaryotic organelles composed of an evolutionary conserved structure of 9 doublet microtubules that are similar but not equivalent. We propose that a tubulin post-translational modification called glutamylation could act […]
Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]
PTR 539 – A multilevel systems approach to elucidate the host-Leishmania interactome and to identify host targets for anti-leishmanial drug discovery
Our PTR project investigates at the complex interface between the intracellular pathogen Leishmania and its macrophage host cell. We will apply systems-wide analyses to elucidate the molecular mechanisms underlying the reciprocal metabolic relationship between […]
Post translational modifications (PTM)
Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most […]
Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a […]
2018Infection Reveals a Modification of SIRT2 Critical for Chromatin Association, Cell Reports 2018, Volume 23, Issue 4:1124-1137.
2018Proteomic analysis of plasma extracellular vesicles reveals mitochondrial stress upon HTLV-1 infection, Sci Rep 2018 Mar;8(1):5170.
2018MUB Binds to Lactoferrin and Stands as a Specific Neutrophil Marker, Cell Chem Biol 2018 Apr;25(4):483-493.e9.
2017Regulation of NF-κB by the p105-ABIN2-TPL2 complex and RelAp43 during rabies virus infection, PLoS Pathog. 2017 Oct;13(10):e1006697.
2017Characterization of Post-Translational Modifications and Cytotoxic Properties of the Adenylate-Cyclase Hemolysin Produced by Various Bordetella pertussis and Bordetella parapertussis Isolates, Toxins (Basel) 2017 Sep;9(10).
2017Outer Membrane Proteome of Veillonella parvula: A Diderm Firmicute of the Human Microbiome, Front. Microbiol., 30 June 2017, 8:6979-17.
2017Outer Membrane Proteome of A Diderm Firmicute of the Human Microbiome, Front Microbiol 2017;8:1215.
2017Proteome remodelling by the stress sigma factor RpoS/σ in Salmonella: identification of small proteins and evidence for post-transcriptional regulation, Sci Rep 2017 May;7(1):2127.
2017Large-Scale SRM Screen of Urothelial Bladder Cancer Candidate Biomarkers in Urine, J. Proteome Res. 2017 04;16(4):1617-1631.
2016Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3, Cell Death Dis 2016 Oct;7(10):e2411.
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