I have been the manager of the Pasteur Proteomics facility since September 2013. I am responsible for the operations of the proteomics platform and the coordination of the different ongoing projects. I did my PhD research in the group of Dr. Bernard Monsarrat and Dr. Odile Schiltz at the Institute of Pharmacology and Structural Biology (IPBS, University Paul Sabatier, UPS, also known as Toulouse III, France). As part of my PhD work, I used high-resolution mass spectrometers, including the LTQ-Orbitrap, in combination with various quantitative proteomic approaches. My focus was on stable isotope labelling strategies (SILAC and ICAT) to study the proteasome inhibitor effect in acute myeloid leukaemia cells. I then moved to the Institute of Molecular Systems Biology in Eidgenössische Technische Hochschule (ETH Zürich) as a post-doctoral fellow in the group of Professor Ruedi Aebersold. I worked on multiple projects using mass spectrometry to assess changes in protein abundance and posttranslational modifications, e.g., ubiquitin, SUMO, and chlorination of proteins using discovery-driven and targeted mass spectrometry. During this time, I utilised the new data independent acquisition mode developed in Ruedi Aebersold Lab, namely SWATH-MS.
Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]
PTR 539 – A multilevel systems approach to elucidate the host-Leishmania interactome and to identify host targets for anti-leishmanial drug discovery
Our PTR project investigates at the complex interface between the intracellular pathogen Leishmania and its macrophage host cell. We will apply systems-wide analyses to elucidate the molecular mechanisms underlying the reciprocal metabolic relationship between […]
Post translational modifications (PTM)
Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most […]
Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a […]
Within cells, proteins are the real effectors of all activities. The expression of certain proteins changes upon cell stimulation or stress (with virus, inhibitors or bacteria), or when cells differentiate or turn into a […]
2017Outer Membrane Proteome of Veillonella parvula: A Diderm Firmicute of the Human Microbiome, Front. Microbiol., 30 June 2017, 8:6979-17.
2016Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3, Cell Death Dis 2016 Oct;7(10):e2411.
2016Determination of differentially regulated proteins upon proteasome inhibition in AML cell lines by the combination of large-scale and targeted quantitative proteomics, Proteomics 2016 Oct;.
2016Protection against malaria in mice is induced by blood stage-arresting histamine-releasing factor (HRF)-deficient parasites, J. Exp. Med. 2016 Jul;.
2016The Oxygen Sensor PHD2 Controls Dendritic Spines and Synapses via Modification of Filamin A, Cell Rep 2016 Mar;14(11):2653-67.
2015Prediction of colorectal cancer diagnosis based on circulating plasma proteins, EMBO Mol Med 2015 Sep;7(9):1166-78.
2015The COPII complex and lysosomal VAMP7 determine intracellular Salmonella localization and growth, Cell. Microbiol. 2015 Dec;17(12):1699-720.
2014Quantitative ADME proteomics – CYP and UGT enzymes in the Beagle dog liver and intestine, Pharm. Res. 2015 Jan;32(1):74-90.
2014A repository of assays to quantify 10,000 human proteins by SWATH-MS., Scientific Data 1 (Nature) Article number: 140031 (2014).
2013Statistical approach to protein quantification, Mol. Cell Proteomics 2014 Feb;13(2):666-77.
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