I have been the manager of the Pasteur Proteomics facility since September 2013. I am responsible for the operations of the proteomics platform and the coordination of the different ongoing projects. I did my PhD research in the group of Dr. Bernard Monsarrat and Dr. Odile Schiltz at the Institute of Pharmacology and Structural Biology (IPBS, University Paul Sabatier, UPS, also known as Toulouse III, France). As part of my PhD work, I used high-resolution mass spectrometers, including the LTQ-Orbitrap, in combination with various quantitative proteomic approaches. My focus was on stable isotope labelling strategies (SILAC and ICAT) to study the proteasome inhibitor effect in acute myeloid leukaemia cells. I then moved to the Institute of Molecular Systems Biology in Eidgenössische Technische Hochschule (ETH Zürich) as a post-doctoral fellow in the group of Professor Ruedi Aebersold. I worked on multiple projects using mass spectrometry to assess changes in protein abundance and posttranslational modifications, e.g., ubiquitin, SUMO, and chlorination of proteins using discovery-driven and targeted mass spectrometry. During this time, I utilised the new data independent acquisition mode developed in Ruedi Aebersold Lab, namely SWATH-MS.
Landscape of protein ubiquitylation during infection by CNF1-producing uropathogenic E. coli
CNF1 is a toxin that catalyzes the permanent activation of host RhoA, Rac1 and Cdc42 small GTPases by selective deamidation of a glutamine residue into a glutamic acid. This 1 dalton post-translational modification can […]
Role of tubulin glutamylation in flagellum construction (TUBGLUFLA)
Cilia and flagella are essential eukaryotic organelles composed of an evolutionary conserved structure of 9 doublet microtubules that are similar but not equivalent. We propose that a tubulin post-translational modification called glutamylation could act […]
Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]
PTR 539 – A multilevel systems approach to elucidate the host-Leishmania interactome and to identify host targets for anti-leishmanial drug discovery
Our PTR project investigates at the complex interface between the intracellular pathogen Leishmania and its macrophage host cell. We will apply systems-wide analyses to elucidate the molecular mechanisms underlying the reciprocal metabolic relationship between […]
Post translational modifications (PTM)
Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most […]
Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a […]
Single Cell Proteomics
Successful extension of mass spectrometry-based (MS) proteomics to single cells is very challenging, mainly due to low analysis throughput and insufficient proteome coverage in single cells. To address these challenges, new pipelines have emerged […]
2020Insights into amebiasis using a human 3D-intestinal model, Cell. Microbiol. 2020 Mar;:e13203.
2020Listeria monocytogenes Exploits Mitochondrial Contact Site and Cristae Organizing System Complex Subunit Mic10 To Promote Mitochondrial Fragmentation and Cellular Infection, mBio 2020 Feb;11(1).
2020Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects, eLife 2020;9:e51998.
2020Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects, Elife 2020 Jan;9.
2019Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche, Cell Rep 2019 Dec;29(12):3958-3973.e7.
2019Assembly and disassembly of Aspergillus fumigatus conidial rodlets, The Cell Surface.
2019Cell-wall synthases contribute to bacterial cell-envelope integrity by actively repairing defects, bioRxiv.
2019Cryptococcus neoformans resists to drastic conditions by switching to viable but non-culturable cell phenotype, PLoS Pathog. 2019 Jul;15(7):e1007945.
2019The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile, Infect. Immun. 2019 08;87(8).
2019Post-translational acylation controls the folding and functions of the CyaA RTX toxin, FASEB J. 2019 Jun;:fj201802442RR.
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