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  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Clinical Research Assistant
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
09
Feb 2015
Status
Ongoing
Members
5
Structures
1

About

Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a bait specific antibody or an affinity reagent specific for an epitope tag fused to the bait protein. Like in co-immunoprecipitation (co-IP) western blot experiments, AP-MS relies on the principle that under native lysis conditions, protein complexes remain largely intact and associated proteins (prey) purify together with the bait protein. The digested peptides of purified complexes are analyzed by shotgun LC-MS/MS and the compiled data of all purification experiments can be assembled to identify the specific interactors.

Scheme: Quantitative mass spectrometry in interaction proteomics
Scheme: Quantitative mass spectrometry in interaction proteomics