I received my Ph.D. in Molecular Chemistry from the Ecole Polytechnique (Palaiseau, France) in 2010 under the supervision of Julia Chamot-Rooke. During this time, I was involved in the development of a top-down mass spectrometry approach combined with hydrogen/deuterium exchange (HDX) for the structural analysis of protein complexes. After my PhD, I joined the proteomics core facility of the Institut Pasteur to work on the Orbitrap Velos ETD coupled with a liquid chromatography. I also manage the high-throughput bottom-up proteomics analysis. I divide my time between the core facility and the Structural Mass Spectrometry and Proteomics Unit headed by Julia Chamot-Rooke, where I am involved in the development of top-down proteomics using high resolution mass spectrometers (Orbitraps).
Cross-linking Mass Spectrometry
Covalent cross-linking combined to mass spectrometry is a powerful approach to address both the structure and composition of protein complexes. To improve the crucial stage of detection and characterization of cross-linked peptides, we have […]
Top-down strategies, based on the study of entire proteins, hold great promise for structural protein analysis. Whilst the classical, bottom-up approach can be useful for locating proteins already deposited in the databases, only a […]
Post translational modifications (PTM)
Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most […]
Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a […]
ProteoCombiner is a high-performance software integrating shotgun with top-down proteomic data. The tool can deal with multiple experiments and search engines. It enables rapid and easy visualization, manual validation, and comparison of the identified […]
2023The cell wall lipoprotein CD1687 acts as a DNA binding protein during deoxycholate-induced biofilm formation in Clostridioides difficile., NPJ Biofilms Microbiomes 2023 May; 9(1): 24.
2023Hemisynthetic alkaloids derived from trilobine are antimalarials with sustained activity in multidrug-resistant Plasmodium falciparum., iScience 2023 Feb; 26(2): 105940.
2023Widespread amyloidogenicity potential of multiple myeloma patient-derived immunoglobulin light chains., BMC Biol 2023 Feb; 21(1): 21.
2022Bacterial Membrane Vesicles as a Novel Strategy for Extrusion of Antimicrobial Bismuth Drug in Helicobacter pylori., mBio 2022 Oct; 13(5): e0163322.
2022A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment., Nat Commun 2022 Jul; 13(1): 4104.
2022NAD kinase promotes Staphylococcus aureus pathogenesis by supporting production of virulence factors and protective enzymes., Elife 2022 Jun; 11(): .
2022A novel screening strategy to identify histone methyltransferase inhibitors reveals a crosstalk between DOT1L and CARM1., RSC Chem Biol 2022 Apr; 3(4): 456-467.
2021Purification of infection-associated macropinosomes by magnetic isolation for proteomic characterization, Nature Protocols, 2021, 16 (11), pp.5220-5249. ⟨10.1038/s41596-021-00610-5⟩.
2021De Novo Sequencing of Antibody Light Chain Proteoforms from Patients with Multiple Myeloma., Anal Chem 2021 08; 93(30): 10627-10634.
2021Advanced In Vivo Cross-Linking Mass Spectrometry Platform to Characterize Proteome-Wide Protein Interactions., Anal Chem 2021 Feb; (): .
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