I received my Ph.D. in Molecular Chemistry from the Ecole Polytechnique (Palaiseau, France) in 2010 under the supervision of Julia Chamot-Rooke. During this time, I was involved in the development of a top-down mass spectrometry approach combined with hydrogen/deuterium exchange (HDX) for the structural analysis of protein complexes. After my PhD, I joined the proteomics core facility of the Institut Pasteur to work on the Orbitrap Velos ETD coupled with a liquid chromatography. I also manage the high-throughput bottom-up proteomics analysis. I divide my time between the core facility and the Structural Mass Spectrometry and Proteomics Unit headed by Julia Chamot-Rooke, where I am involved in the development of top-down proteomics using high resolution mass spectrometers (Orbitraps).
Cross-linking Mass Spectrometry
Covalent cross-linking combined to mass spectrometry is a powerful approach to address both the structure and composition of protein complexes. To improve the crucial stage of detection and characterization of cross-linked peptides, we have […]
Top-down strategies, based on the study of entire proteins, hold great promise for structural protein analysis. Whilst the classical, bottom-up approach can be useful for locating proteins already deposited in the databases, only a […]
Post translational modifications (PTM)
Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most […]
Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a […]
ProteoCombiner is a high-performance software integrating shotgun with top-down proteomic data. The tool can deal with multiple experiments and search engines. It enables rapid and easy visualization, manual validation, and comparison of the identified […]
2021De Novo Sequencing of Antibody Light Chain Proteoforms from Patients with Multiple Myeloma., Anal Chem 2021 08; 93(30): 10627-10634.
2021Advanced In Vivo Cross-Linking Mass Spectrometry Platform to Characterize Proteome-Wide Protein Interactions., Anal Chem 2021 Feb; (): .
2021Molecular basis of the interaction of the human tyrosine phosphatase PTPN3 with the hepatitis B virus core protein., Sci Rep 2021 Jan; 11(1): 944.
2021Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus., Front Immunol 2021 ; 12(): 677798.
2020ProteoCombiner: integrating bottom-up with top-down proteomics data for improved proteoform assessment., Bioinformatics 2020 Nov; (): .
2020Optimization of a Top-Down Proteomics Platform for Closely Related Pathogenic Bacterial Discrimination., J Proteome Res 2020 Oct; (): .
2020Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape., PLoS Pathog 2020 08; 16(8): e1008822.
2020The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis., Nat Commun 2020 04; 11(1): 1941.
2020Infection-driven activation of transglutaminase 2 boosts glucose uptake and hexosamine biosynthesis in epithelial cells., EMBO J 2020 04; 39(8): e102166.
2019Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche., Cell Rep 2019 12; 29(12): 3958-3973.e7.
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