Proteomics

ProteoCombiner is a high-performance software integrating shotgun with top-down proteomic data. The tool can deal with multiple experiments and search engines. It enables rapid and easy visualization, manual validation, and comparison of the identified […]

Intracellular bacterial pathogens enter host cells to form a bacterial containing vacuole (BCV). Then, the BCV matures or ruptures leading to two distinct pathogen-dependent intracellular niches; one vacuolar and one cytosolic. The specific intracellular […]

A common phenomenon in most microbial infections is the attenuation of the host’s immediate immune response, allowing for long-term colonization. We and others have shown that many pathogens achieve this by manipulating the epigenetic […]

This project is a part of the international Infect-Era program, AMOEBAC: Entamoeba histolytica-bacterium interaction and the role of this interaction in intestinal pathogenesis (http://www.infect-era.eu/2nd-call-2014) The aim of this investigation is to understand the induced […]

CNF1 is a toxin that catalyzes the permanent activation of host RhoA, Rac1 and Cdc42 small GTPases by selective deamidation of a glutamine residue into a glutamic acid. This 1 dalton post-translational modification can […]

Cilia and flagella are essential eukaryotic organelles composed of an evolutionary conserved structure of 9 doublet microtubules that are similar but not equivalent. We propose that a tubulin post-translational modification called glutamylation could act […]

Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]

This PTR aims to gain novel insight into the nature, the expression, and the regulation of Leishmania virulence factors through the investigation of genetics, transcriptomics, and proteomics differences between (i) virulent and avirulent L. […]

Post translational modifications (PTMs) are known to play pivotal roles in cellular physiology and disease, but only a few of the over 300 currently known modifications are extensively investigated in proteomic studies. The most […]

Affinity Purification Mass Spectrometry (AP-MS) is the method of choice to study protein-protein interactions (PPI) and complexes under near physiological conditions. A protein of interest – the bait – is purified by either a […]

Within cells, proteins are the real effectors of all activities. The expression of certain proteins changes upon cell stimulation or stress (with virus, inhibitors or bacteria), or when cells differentiate or turn into a […]

Cytokinesis leads to the physical separation of daughter cells and concludes cell division. Final abscission occurs close to the midbody (or Flemming body), a prominent structure that matures at the center of the intercellular […]