Welcome to the web site of the Trypanosome Cell Biology Unit (TRYPA)!
Our lab is studying the role and the functioning of the trypanosome flagellum, with perspectives in the field of both parasitology and genetic diseases. Indeed, trypanosomes are significant parasites of man and cattle in central Africa and there are currently no efficient vaccines against them. Moreover, trypanosomes are also an excellent model to study human genetic diseases due to defects in cilia and flagella. Our lab is an international mixture with 6 nationalities and expertise in parasitology and cell biology. We are a Pasteur full research unit and are affiliated to the Department of Parasites and Insect Vectors (headed by Gerald Spaeth) but also to the Department of Cell Biology and Infection (headed by Marc Lecuit). We belong to an INSERM unit (U1201, headed by Artur Scherf) that involves two other teams working on Plasmodium and on Leishmania. We are also part of the LabEx IBEID, a consortium whose aim is to develop a structure to anticipate and tackle emerging infectious diseases. Recently we have been accepted as a new member of the Labex ParaFrap. We live on the first floor of the Nicolle building but are travelling regularly through the multiple platforms of the campus.
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VIDEO: Click here for a general presentation of sleeping sickness (in French)
01/08/2022. A little party to celebrate our “Equipe FRM” grant! Congratulations to all contributors and thank you FRM!
01/07/2022. Adeline’s image of segmented trypanosome flagella is on the front cover of Bioessays!
18/05/2021. Estefania’s paper on FLAM8 distribution during the trypanosome life cycle is now available on line: https://onlinelibrary.wiley.com/doi/10.1111/cmi.13347
17/05/2021. Superb front cover from our friend Hassan Hashimi! 01/03/2021. Seb’s paper on gene expression in parasites from the salivary glands is now available on BioRxiv. Feedback is welcome! 24/02/2021. Very happy that our FIB-SEM data could be exploited for analysis of the trypanosome mitochondrion! Link here to download the paper. 03/12/2020. Congratulations to Adeline Mallet who successfully defended her thesis yesterday!
11/11/2020 First molecular insights in the locking mechanisms now published in JCB! Great collaboration with the lab of Sue Vaughan at Oxford Brookes University! 31/08/2020. Eloïse’s paper on IFT in different life cycle stages of trypanosomes is now published in JCS. It was a real “tour de force” to deal with these wiggling parasites! This video showing FRAP to evaluate the dynamics of IFT proteins at the tip of the flagellum of short epimastigote cells is particularly stunning! Video FRAP 15/07/2020. We have received more than 100 applications for the PhD position. Many thanks to those who applied, we are going to interview candidates very soon. 30/06/2020. We said goodbye to Estefania who spent 5 years in the lab under the supervision of Brice. We thank her for the hard work and the constant enthusiasm and wish her all the best for the future. 06/04/2020. Moara’s work is now published in Parasites and Vectors. 20/01/2020. Our review on how cells deal with different flagella is now on line. We got stimulating feedback from colleagues. See our twitter account for more details! 2020. Happy New Year! 21st November. Welcome to Ramiro Tomasina from the lab of Maria Francia at @IPMontevideo who is going to spend one month in the lab studying basal body proteins with us. 6th November. Thierry was awarded “La médaille d’or d’honneur du travail” for his 35 years at Institut Pasteur! 14th October. Congratulations to Moara who is moving to a new job (permanent position!) as a research engineer in the lab of Dorit Hanein. She had great contributions on IFT kinesin, trypanosome development in the fly and in general in electron microscopy. We’ll miss her! September. Moara’s work on flagellum formation in trypanosomes within the proventriculus of tsetse flies is available on BioRxiv. It was a serendipitous but thrilling discovery: read the full story in this thread. 2nd September. We are delighted that two former lab members are opening their own research groups: Sabrina Absalon in Indianapolis and Diego Huet in Athens! 1st July. Congratulations to Brice who has been promoted Director of Research! 14th June. We are delighted that our lab has been accepted as new member of the Parafrap consortium! 30th April. Adeline’s review discussing the possible explanations for IFT train positioning is out: https://www.medecinesciences.org/fr/articles/medsci/abs/2019/05/msc190032/msc190032.html … It is a follow-up of our recent JCB paper http://jcb.rupress.org/content/217/12/4284 … and was a good exercice to get her ready for the discussion of her thesis! 25th April. Our unit is renewed for another 10-year term! 2nd April. Our work to decipher how IFT22 contributes to IFT has just been published! In a great collaboration with Esben Lorentzen, we showed unconventional nucleotide binding and identify the interface with the rest of the IFT-B complex. The paper is accessible here. 28th March. Happy to see the study of our collaborator Penny Smirlis in press. She came to the lab some time ago to work out the localisation of GSK3 and then did a nice job in developing indirubin analogues that inhibit T. brucei growth at nanomolar concentration. 11th February. Great news, the Labex IBEID is renewed for 5 years! 21st January. The IFT25 paper has been accepted for publication in Journal of Cell Science. HuetHuet-VideoS2-JCS 1st December. Our work on IFT25 is now available on BioRxiv. 15th November. The work of Eloïse and Benjamin (former postdoc) describing a new model for flagellum length control has been published in Current Biology. More details can be found on our Twitter account. 7th November. Prachee Avasthi wrote a nice spotlight in JCB on our IFT paper. 2nd November. Serge’s paper on the intriguing ODA8/LRRC56 protein is out in American Journal of Human Genetics. It was a great collaboration with the clinical teams of Eamonn Sheridan and Kym Boycott, made possible thanks to Michel Leroux. More details on our Twitter account. 27th October. We have opened our Twitter account. It can also be reached from the icon above. 16th October. We got a prize for our cycling efforts to raise funding for the Institut Pasteur! 11th October. We had the visit of Professor Liangyi Chen from Peking University. He gave an impressive talk on the recent developments he achieved in super-resolution imaging of live cells. The potential of these approaches for imaging IFT is super-exciting! 10th October. Today was the kick-off meeting for our new project on tubulin polyglutamylation with the units of Julia Chamot-Rooke and Carsten Janke. We already made a lot of progress through a deep brainstorming session! 3rd October. Serge’s paper on the function of LRRC56 in collaboration with Eamonn Sheridan’s group is accepted in American Journal of Human Genetics. It is currently available on line in BioRxiv. 2nd October. The IFT distribution paper is now available on the web site of JCB. 21st September. Our paper on the positioning of IFT in the trypanosome flagellum has been accepted in Journal of Cell Biology! It is currently available on line in bioRxiv. 20th September. Eloïse has completed her thesis defence and is a now a doctor! She had stimulating discussion with the jury composed of cilia, cytoskeleton and trypanosome people. We thank them for the excellent questions and for having stimulated such an exciting debate. Eloïse will stay with us for a short post-doc until the end of the year. 13th July. Great news, our ANR grant to study tubulin glutamylation has been funded! 23rd May 2018. Our work on the identification of two tracks for IFT in the trypanosome flagellum is now available on line in bioRxiv. 12th April. Moara won an award for best poster presentation at the departmental retreat in Maintenon! 27th March. Serge’s work on the intriguing ODA8/LRRC56 is now on line on bioRxiv. 16th February. The work of Eloïse and Benjamin (former postdoc) describing a new model for flagellum length control is now available on bioRxiv. 5th-8th February. We spent three excellent days at Strasbourg for the lab retreat. It is amazing how much work can be done when we focus about it in a tiny cosy room! While Paris was under the snow, we enjoyed dry weather. Unexpected! 2nd February. Thierry’s beautiful image makes the front cover of this month issue of Biology of the Cell!
31st January. Seb is awarded a Marie Curie postdoctoral fellowship to develop his project on single cell analysis! 15th December. Sylvain’s paper describing the structure of the transition zone by STEM has been accepted for publication and can be accessed here. It reveals two domains for the TZ and a more sophisticated organisation of the Y-links than initially thought. OTHER NEWS: see bottom of the page