© Christine Schmitt, Anubis Vega Rua, Jean-Marc Panaud
Tête de moustique femelle Aedes albopictus, vecteur du virus de la dengue et du chikungunya. Microphotographie électronique à balayage, image colorisée.
Laboratory

Arboviruses and Insect Vectors

About

The research developed in our unit “Arboviruses and Insect vectors” aims to understand the potential of arboviruses to emerge by dissecting the vectorial system of different combinations of viruses/blood-feeding insects.

The laboratory “Arboviruses and Insect Vectors” has been transformed into a Unit of Research and Expertise (URE) – Network on November 1st 2014 in the Department of Virology at the Institut Pasteur in Paris. Dr Anna-Bella Failloux is the leader of this unit.

In this context, our unit has set some of its objectives of research with the support of the International Network of Institut Pasteur (INIP). This global network of 32 institutes on 5 continents is uniquely positioned to play a major role both in furthering our understanding of vector borne diseases and the challenge of controlling them. Our unit relies on the field experience offered by our collaborators within the INIP to anchor our projects and multiple trips between the field and laboratory are necessary in order to test hypotheses and direct future research.

Arthropod-borne viruses (arboviruses) are transmitted among vertebrate hosts by hematophagous arthropod vectors such as mosquitoes. Vertebrates are “blood-sources” required by this kind of arthropods to complete their life cycle. Blood providing nutrients for ovogenesis, females must feed on host to be able to mature their eggs. In the course of this blood-meal, saliva is injected and transmission of the pathogen to the vertebrate can occur if infectious particles are present in saliva. Blood-feeding arthropods may feed several times during their life span and can ingest genetically distinct variants of a given virus species playing a key role in generating and maintaining genetic diversity and in selecting genotypes involved in epidemics.

Our laboratory hosts the collection of arthropods of the Institut Pasteur : http://www.pasteur.fr/arthropodes/

Program Projects

Projects

Publications

Poste d'ingénieur d'étude

Poste d’ingénieur d’étude au Laboratoire “Arbovirus et Insectes Vecteurs” à l’Institut Pasteur à Paris

L’Institut Pasteur, une fondation privée à but non lucratif qui se consacre principalement à l’étude des maladies infectieuses, recrute un ingénieur d’étude de niveau Bac +5. Il sera affecté à l’unité « Arbovirus et Insectes Vecteurs » dirigée par Anna-Bella FAILLOUX. Ce laboratoire étudie la transmission des arbovirus par les moustiques vecteurs. Les modèles d’étude concernent les vecteurs du chikungunya, dengue, Zika, West-Nile, fièvre jaune et fièvre de la Vallée du Rift. Ces études nécessitent des infrastructures adaptées à la manipulation des moustiques infectés. Pour plus d’information, veuillez visiter notre site : https://research.pasteur.fr/fr/team/arboviruses-and-insect-vectors/.

Description

Le candidat devra caractériser des populations du moustique tigre, Aedes albopictus qui est une espèce invasive d’origine asiatique. A la faveur de l’intensification des échanges commerciaux intercontinentaux, cette espèce s’est propagée hors de ses territoires d’origine. Il a été démontré que le génome de cette espèce présente une insertion d’un fragment présentant une forte homologie avec un flavivirus, que l’on a appelé « Cell Silent Agent (CSA) ». Cet ORF de de 1557 acides aminés est phylogénétiquement proche des séquences couvrant la région NS1-NS4A des flavivirus d’insectes. Nous nous interrogeons sur le rôle de cette séquence dans la transmission des flavivirus tels que le virus de la dengue. Le candidat devra : (i) déterminer le polymorphisme génétique de populations d’Ae. albopictus provenant de différentes zones géographiques, par génotypage au moyen de microsatellites ou autres marqueurs et (ii) définir le polymorphisme de la séquence CSA intégrée dans le génome de ces mêmes populations.

 Qualification

Le candidat devra présenter des aptitudes en biologie moléculaire, phylogénie et bio-informatique sans phobie marquée pour les moustiques.

Information complémentaire

Financement: LabEx IBEID

Début de contrat: Octobre 2016 (durée de 18 mois)

Lieu: Paris, France

Les candidats devront envoyer à anna-bella.failloux@pasteur.fr: une lettre d’intention, un CV détaillé, et le nom de deux références.

La date limite de la soumission est le 31 aout 2016.

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Anna-Bella FAILLOUX

Institut Pasteur, Department of Virology, Arboviruses and Insect Vectors

25-28 rue du Dr Roux, 75724 Paris cedex 15

Phone: 33-1-40613617 ; E-mail: anna-bella.failloux@pasteur.fr

 

 

PhD offer

OFFER FOR A PhD in the Laboratory “Arboviruses and Insect Vectors” at the Institut Pasteur in Paris

The Institut Pasteur, a private, non-profit foundation for biomedical research, is looking for a doctoral fellow in the Laboratory “Arboviruses and Insect Vectors” headed by Dr Anna-Bella FAILLOUX. The host lab provides state-of-the-art facilities including a BSL-3 dedicated to experimental infections of mosquitoes. This know-how is the heart of our projects: emergence of arboviruses due to invasive vectors, influence of environmental factors (temperature, insecticides..) on viral transmission, design of new technologies for vector control (genetically modified mosquitoes) (please visit our website: https://research.pasteur.fr/fr/team/arboviruses-and-insect-vectors/). The lab is also part of the IBEID LabEx (Laboratory of Excellence, labelled by the Ministry of Research).

Description

The insect-specific flaviviruses (ISF) have been neglected until recently due to their inability to infect vertebrate cells. However, some ISFs have been shown to modulate the replication of arboviruses of the Flavivirus genus. Interestingly, a cell silent agent (CSA) has been found integrated as a single copy in the genome of the mosquito Aedes albopictus. This sequence shown to encode a NS1-NS4A-like transcript may have been integrated in the mosquito genome due to endogenous reverse transcriptase and integrase activities. The role and impact of CSA sequences on transmission of arboviruses by mosquito vectors are still widely unexplored. Our project aims to precise the part of CSA sequences on transmission of an homologous flavivirus, dengue and the alphavirus chikungunya by Ae. albopictus knowing that superinfection in host cells with genetically related viruses can be constrained. The applicant will be involved in determining whether the presence of CSA alters the transmission of dengue and chikungunya viruses, and explore the role of CSA in priming the mosquito immune responses with consequences on transmission of arboviruses.

Qualification

The position is ideally suited for highly motivated applicants with a strong background in molecular virology. The ideal candidate should be highly motivated, curious and enthusiastic to work in a collaborative team. French language is not a requirement. Strong written and oral communication skills in English are required.

Additional Information

Funding: European Research Council

Starting date: October 2016 (duration 36 months)

Location: Paris, France

Applicants should send a letter of intent, a detailed CV, a summary of their previous research activities (≤ 1 page), and the names of two references to anna-bella.failloux@pasteur.fr

Dead line for submission: August, 31th 2016 (later applications will be considered until the position is filled).

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Anna-Bella FAILLOUX

Institut Pasteur, Department of Virology, Arboviruses and Insect Vectors

25-28 rue du Dr Roux, 75724 Paris cedex 15

Phone: 33-1-40613617 ; E-mail: anna-bella.failloux@pasteur.fr

Contact

Phone: + 33 (0)1 40 61 36 17
Email: anna-bella.failloux@pasteur.fr

Address
25-28 Rue du Docteur Roux
75015, Paris
France