Biofilms are communities of microorganisms that interact with surfaces, and are known to display unique properties that differentiate them from free-floating, individual microorganisms. While biofilms are widespread and play many positive roles in all environments, they are also difficult to eradicate when adhered to surfaces in medical and industrial settings.We use genetics, genomics, molecular biology, and various in vitro and in vivo biofilm models to better understand biofilm-associated functions used by commensal and pathogenic bacteria. We also attempt to translate our fundamental approaches into relevant anti-biofilm strategies in collaboration with clinicians and industrial partners.
Group: Christophe Beloin
BACTERIAL STRUCTURES PROMOTING BIOFILM FORMATION Commensal and pathogenic bacteria such as Escherichia coli produce various surface structures promoting their interaction with living or inert surfaces. We are particularly interested in identifying E. coli adhesins that are poorly expressed in […]
• Bacterial Interactions in in vitro BiofilmsAlthough competitive interactions between bacteria (bacterial interference) are likely to represent a key aspect of many infectious processes, the nature of these interactions within mixed biofilm communities is […]
IDENTIFICATION OF BIOFILM-SPECIFIC MOLECULES Specific metabolic changes occurring during biofilm formation were hypothesized to lead to the production of biofilm-associated molecules and biofilm-specific functions. The identification of biofilm-specific metabolites could reveal new aspects of biofilm biology, and lead to […]
Biofilm Tolerance to Antibiotics
BIOFILM-ASOCIATED TOLERANCE TO ANTIBIOTICS One of the of the hallmarks of biofilm physiology is its tolerance to a high level of antimicrobials. We are currently investigating the molecular mechanisms that lead to this transient ability to […]
Biofilm Volatile Compounds
Bacteria produce and release into their headspace a wide variety of volatile secondary metabolites. However, While detection and quantification of these volatiles as attractive or repellent odors and aromas are of great interest in food and cosmetic bioprocesses, […]
Biofilm formation on medical devices is associated with hospital-acquired (nosocomial) infections. Due to biofilms’ high tolerance to antibiotics, classical treatments lack efficacy, and removal of the biofilm-contaminated device is often the only therapeutic option. […]
Fighting Antibiotic Resistance
Antibiotics are highly efficient drugs to kill pathogenic bacteria with low side effects. For more than 70 years, the massive use of these miracle drugs has saved millions of human and animal lives. Unfortunately […]
Inception – Institut Convergence for the study of Emergence of Pathology Through Individuals and Populations
Inception Goal The Inception’s goal is to develop a core structure to mobilize data resources, numerical sciences, and fundamental experimental biology in a range of health issues. Inception program uses Integrative Biology, Social Science […]
Genetic Methods and Tools
We originally developed genetic tools to study adhesion and biofilm formation in commensal and pathogenic E. coli species. These tools are made freely available to the scientific community upon request.• pKOBEG Lambda Red plasmidsBased […]
In vivo Study of Biofilm-Associated Catheter Infections
We developed a clinically relevant in vivo model of catheter-associated biofilm infections to design new and efficient biofilm-eradication approaches applicable to contaminated totally implantable venous access port (TIVAP) catheters. Key publication• Chauhan, A., D. Lebeaux, B. Decante, I. Kriegel, M.C. […]
We designed a continuous-flow culture system in which biofilm formation can be reproducibly monitored and controlled in microfermentors handled at flow-rates avoiding accumulation of planktonic bacteria. This model is suited for biofilm macroscopic analysis, […]
2017Comparative Analysis of Bacterial Community Composition and Structure in Clinically Symptomatic and Asymptomatic Central Venous Catheter., mSphere, in press.
2017Biofilm microenvironment induces a widespread adaptive amino-acid fermentation pathway conferring strong fitness advantage in Escherichia coli, PLoS Genetics, In Press.
2017Central venous catheters and biofilms: where do we stand in 2017?, APMIS. 2017 Apr;125(4):365-375. doi: 10.1111/apm.12665..
2017Understanding biofilm formation in intravascular device-related infections, Intensive Care Medicine. Mar;43(3):443-446. doi: 10.1007. [Epub 2016 Aug 6].
2017Biological and chemical strategies for exploring inter- and intrakingdom communication mediated via bacterial volatile signals., Nat. Protoc. in press.
2016Phylogenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes, Elife. 2016 Aug 31;5. pii: e14589..
2016Functional analysis of Escherichia coli Yad fimbriae reveals their potential role in environmental persistence, Environ Microbiol. 2016 18(12);5228-5248.
2016Impact of percutaneous pulmonary valve implantation procedural steps on leaflets histology and mechanical behaviour: An in vitro study., Arch Cardiovasc Dis. 2016 Aug.-Sep;109(8-9)465-475 [Epub 2016 Jun 23].
2016Ultimate single-copy DNA detection using real-time electrochemical LAMP, ACS Sensors 1(7); 904-912.
2016Biofilms 2015: Multi-disciplinary approaches shed light into microbial life on surfaces, J Bacteriol. 2016 Mar 14. pii: JB.00156-16. [Epub ahead of print].
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