Microbiology Course Institut Pasteur
I have been appointed in 2010 then Co-director together with Dr Françoise Norel-Bozouklian in 2014 of this Microbiology course. This nine-week theorical and practical course presents the latest advances in molecular and cellular microbiology. […]
Institut Pasteur Microbiology Course
Françoise Norel has been appointed Co-director of the Institut Pasteur Microbiology Course in 2014. This course is held annually and is part of master trainings proposed by 3 universities: Pierre and Marie Curie (Paris […]
The outer membrane (OM) is an essential element in Gram-negative (diderm) bacteria. Its integrity is maintained by the control of lipids asymmetry via LPS and phospholipids transport by the Lpt and Mla systems, respectively. […]
Biofilms formed by anaerobes
Although many clinically and ecologically relevant processes are known to be performed by biofilm communities composed of strict or facultative anaerobic bacteria, biofilms were often studied in aerobic conditions. We use in vitro anaerobic culture systems […]
ANR Fir-OM: Firmicutes with an outer membrane: toward new models to study the diderm/monoderm cell envelope transition
The bacterial envelope is one of the oldest and most essential cellular components, being involved in key housekeeping functions, such as physical integrity, cell division, motility, substrate uptake and secretion, and cell-cell communication. It […]
PTR OmNega: A multi-disciplinary investigation of the Negativicutes: atypical Firmicutes with LPS-outer membranes that inhabit the human gut
The Negativicutes are a poorly studied lineage of bacteria that include common inhabitants of the human oral and gut microbiome such as the anaerobe Veillonella, and which can also develop into opportunistic pathogens. Despite […]
Commensal and pathogenic bacteria such as Escherichia coli produce various surface structures promoting their interaction with living or inert surfaces. We study the structure and regulation of E. coli adhesins and extracellular matrix polysaccharides contributing to biofilm […]
Specific metabolic changes occurring during biofilm formation were hypothesized to lead to the production of biofilm-associated molecules and biofilm-specific functions. The identification of biofilm-specific metabolites could reveal new aspects of biofilm biology, and lead to novel strategies […]
Biofilm Tolerance to Antibiotics
One of the of the hallmarks of biofilm physiology is its tolerance to a high level of antimicrobials. We are currently investigating the molecular mechanisms that lead to this transient ability to withstand antimicrobial treatments. […]
Biofilm formation on medical devices is associated with hospital-acquired (nosocomial) infections. Due to biofilms’ high tolerance to antibiotics, classical treatments lack efficacy, and removal of the biofilm-contaminated device is often the only therapeutic option. […]
Biophysics of Bacteria-Surface Interactions
Biofilm formation relies as much on bacterial adhesion properties than on surface chemistry, and many questions remain regarding biophysics of surface adhesion. In collaboration with physico-chemists and biophysicists, we used multidisciplinary approaches to investigate bacterial […]
Genetic Methods and Tools
We originally developed genetic tools to study adhesion and biofilm formation in commensal and pathogenic E. coli species. These tools are made freely available to the scientific community upon request. • pKOBEG Lambda Red […]
In vivo Study of Biofilm-Associated Catheter Infections
We developed a clinically relevant in vivo model of catheter-associated biofilm infections to design new and efficient biofilm-eradication approaches applicable to contaminated totally implantable venous access port (TIVAP) catheters. Key publications • Chauhan, A., J.M. […]
We designed a continuous-flow culture system in which biofilm formation can be reproducibly monitored and controlled in microfermentors handled at flow-rates avoiding accumulation of planktonic bacteria. This model is suited for biofilm macroscopic analysis, […]
2022An ancient divide in outer membrane tethering systems in bacteria suggests a mechanism for the diderm-to-monoderm transition., Nat Microbiol 2022 Mar; 7(3): 411-422.
2021Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress., NPJ Biofilms Microbiomes 2021 Apr; 7(1): 34.
2021On the strong connection between nanoscale adhesion of Yad fimbriae and macroscale attachment of Yad-decorated bacteria to glycosylated hydrophobic and hydrophilic surfaces, Nanoscale . 2021 Jan 14;13(2):1257-1272. doi: 10.1039/d0nr06840c. Epub 2021 Jan 6. .
2021Speciality Grand Challenge for “Biofilms”., Front Cell Infect Microbiol 2021 ; 11(): 632429.
2020Genome-wide analysis of the Firmicutes illuminates the diderm/monoderm transition., Nat Ecol Evol 2020 12; 4(12): 1661-1672.
2020Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST, Sci Rep. 2020 Sep 25;10(1):15791. doi: 10.1038/s41598-020-72498-2. .
2020Autotransporters drive biofilm formation and auto-aggregation in the diderm Firmicute Veillonella parvula, J Bacteriol . 2020 Aug 17;JB.00461-20. doi: 10.1128/JB.00461-20. Online ahead of print. .
2020Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation., mBio 2020 06; 11(3): .
2020Capsular polysaccharide cross-regulation modulates Bacteroides thetaiotaomicron biofilm formation, mBio May/June 2020 Vol.11(3): e00729-20.
2020One or two membranes? Diderm Firmicutes challenge the Gram-positive/Gram-negative divide., Mol Microbiol 2020 03; 113(3): 659-671.
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