I prepared my PhD in the laboratory of Structure and Expression of Bacterial Genome directed by Dr. Françoise Le Hégarat at the University Paris XI where I studied histone-like proteins of the Gram positive bacteria Bacillus subtilis. I obtained my PhD in 1998. The same year I joined the laboratory of Prof. Charles J. Dorman in Trinity College Dublin as a European Post-doctoral fellow. During these three years my research has been focused on the regulation of virulence gene expression in the pathogenic bacteria Shigella flexneri. In 2001, I came back to France on a postdoctoral proposition in the 5 years group recently created by Dr. Jean-Marc Ghigo. I participated to the implementation of the G5 Genetics of Biofilms Laboratory. In 2003, I was recruited at the Institut Pasteur as Assistant Professor (Chargé de Recherche) and have been promoted Associate Professor in 2014. In the Ghigo’s laboratory, my work focuses on two biofilm-specific phenomena: initial adhesion to surfaces or between bacteria, which is a key step of any colonization process, and biofilms recalcitrance towards antibiotic , a major biofilm behavior responsible for chronic and recurrent infections in clinic. Beyond the importance of a better fundamental understanding of the molecular mechanisms controlling these two biofilm-properties , these studies have also been motivated by my desire to translate, when possible, some aspects of my work into clinically relevant situations involving biofilms. Since 2011 I’m strongly involved in teaching at the Institut Pasteur and I have been appointed Co-director of the Institut Pasteur Microbiology Course in 2014.
Microbiology Course Institut Pasteur
I have been appointed in 2010 then Co-director together with Dr Françoise Norel-Bozouklian in 2014 of this Microbiology course.This nine-week theorical and practical course presents the latest advances in molecular and cellular microbiology. Training […]
Institut Pasteur Microbiology Course
Françoise Norel has been appointed Co-director of the Institut Pasteur Microbiology Course in 2014. This course is held annually and is part of master trainings proposed by 3 universities: Pierre and Marie Curie (Paris […]
ANR Fir-OM: Firmicutes with an outer membrane: toward new models to study the diderm/monoderm cell envelope transition
Firmicutes with an outer membrane: toward new models to study the diderm/monoderm cell envelope transition The bacterial envelope is one of the oldest and most essential cellular components, being involved in key housekeeping functions, […]
PTR OmNega: A multi-disciplinary investigation of the Negativicutes: atypical Firmicutes with LPS-outer membranes that inhabit the human gut
The Negativicutes are a poorly studied lineage of bacteria that include common inhabitants of the human oral and gut microbiome such as the anaerobe Veillonella, and which can also develop into opportunistic pathogens. Despite […]
BACTERIAL STRUCTURES PROMOTING BIOFILM FORMATION Commensal and pathogenic bacteria such as Escherichia coli produce various surface structures promoting their interaction with living or inert surfaces. We are particularly interested in identifying E. coli adhesins that are poorly expressed in […]
IDENTIFICATION OF BIOFILM-SPECIFIC MOLECULES Specific metabolic changes occurring during biofilm formation were hypothesized to lead to the production of biofilm-associated molecules and biofilm-specific functions. The identification of biofilm-specific metabolites could reveal new aspects of biofilm biology, and lead to […]
Biofilm Tolerance to Antibiotics
BIOFILM-ASOCIATED TOLERANCE TO ANTIBIOTICS One of the of the hallmarks of biofilm physiology is its tolerance to a high level of antimicrobials. We are currently investigating the molecular mechanisms that lead to this transient ability to […]
Biofilm formation on medical devices is associated with hospital-acquired (nosocomial) infections. Due to biofilms’ high tolerance to antibiotics, classical treatments lack efficacy, and removal of the biofilm-contaminated device is often the only therapeutic option. […]
Genetic Methods and Tools
We originally developed genetic tools to study adhesion and biofilm formation in commensal and pathogenic E. coli species. These tools are made freely available to the scientific community upon request.• pKOBEG Lambda Red plasmidsBased […]
In vivo Study of Biofilm-Associated Catheter Infections
We developed a clinically relevant in vivo model of catheter-associated biofilm infections to design new and efficient biofilm-eradication approaches applicable to contaminated totally implantable venous access port (TIVAP) catheters. Key publication• Chauhan, A., D. Lebeaux, B. Decante, I. Kriegel, M.C. […]
We designed a continuous-flow culture system in which biofilm formation can be reproducibly monitored and controlled in microfermentors handled at flow-rates avoiding accumulation of planktonic bacteria. This model is suited for biofilm macroscopic analysis, […]
2017Comparative Analysis of Bacterial Community Composition and Structure in Clinically Symptomatic and Asymptomatic Central Venous Catheter., mSphere, in press.
2017Central venous catheters and biofilms: where do we stand in 2017?, APMIS. 2017 Apr;125(4):365-375. doi: 10.1111/apm.12665..
2017Understanding biofilm formation in intravascular device-related infections, Intensive Care Medicine. Mar;43(3):443-446. doi: 10.1007. [Epub 2016 Aug 6].
2016Functional analysis of Escherichia coli Yad fimbriae reveals their potential role in environmental persistence, Environ Microbiol. 2016 18(12);5228-5248.
2016Phylogenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes, Elife. 2016 Aug 31;5. pii: e14589..
2016Impact of percutaneous pulmonary valve implantation procedural steps on leaflets histology and mechanical behaviour: An in vitro study., Arch Cardiovasc Dis. 2016 Aug.-Sep;109(8-9)465-475 [Epub 2016 Jun 23].
2016Study of in vivo catheter biofilm infections using pediatric central venous catheter implanted in rat, Nature Protocol 2016 Mar;11(3):525-41Epub 2016 Feb 18.
2015Selective propensity of bovine jugular vein material to bacterial adhesions: An in-vitro study., Int J Cardiol. 2015 Nov. 1;198:201-205. [Epub ahead of print Jul. 6].
2015In vitro activity of gentamicin, vancomycin or amikacin combined with EDTA or l-arginine as lock therapy against a wide spectrum of biofilm-forming clinical strains isolated from catheter-related infections, J. Antimicrob. Chemother. 2015 Jun;70(6):1704-12.
2015Inhibition of type 1 fimbriae-mediated Escherichia coli adhesion and biofilm formation by trimeric cluster thiomannosides conjugated to diamond nanoparticles., Nanoscale. 2015 Feb 14;7(6):2325-35.
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