I was trained as a high-school biology teacher, but I got sidetracked and, instead, I started research as a undergraduate student in 1990 with Cécile Wandersman ( In memoriam) at the Institut Pasteur, Paris, France (type 1 protein secretion and haem acquisition in Gram-negative bacteria). I then obtained my PhD in 1994. While appointed Research Assistant at the Institut Pasteur in 1995, I took a 2-year post-doctoral leave of absence in 1996 to join the laboratory of Jon Beckwith at Harvard Medical School, Boston, USA, (cell division in Escherichia coli). I went back to the Institut Pasteur in 1999 to develop an independent project investigating bacterial biofilm lifestyle at the genetic and molecular level. This led to the creation of the 5 year young investigator Genetics of Biofilms group (G5-2002 to 2007) and the subsequent creation of the Genetic of Biofilms Unit (2007-present). I am currently Professor at the Institut Pasteur and Deputy Director of the Department of Microbiology at the Institut Pasteur.
BACTERIAL STRUCTURES PROMOTING BIOFILM FORMATION Commensal and pathogenic bacteria such as Escherichia coli produce various surface structures promoting their interaction with living or inert surfaces. We are particularly interested in identifying E. coli adhesins that are poorly expressed in […]
• Bacterial Interactions in in vitro BiofilmsAlthough competitive interactions between bacteria (bacterial interference) are likely to represent a key aspect of many infectious processes, the nature of these interactions within mixed biofilm communities is […]
When Gut Bacteria Changes Brain Functions
We are interested in deciphering the relationships that link gut microbiota with the immune system and the brain. So far, the mechanisms underlying these interplay, and most importantly their consequences, remain largely unknown. Therefore […]
IDENTIFICATION OF BIOFILM-SPECIFIC MOLECULES Specific metabolic changes occurring during biofilm formation were hypothesized to lead to the production of biofilm-associated molecules and biofilm-specific functions. The identification of biofilm-specific metabolites could reveal new aspects of biofilm biology, and lead to […]
Bacteria produce hundreds of volatile compounds, important for the crafting of microbe-based foods like wine, beer, or cheese. Although the odorant chemicals emitted by bacteria were rarely characterized in the context of bacteria-bacteria or bacteria-host […]
Biofilm Volatile Compounds
Bacteria produce and release into their headspace a wide variety of volatile secondary metabolites. However, While detection and quantification of these volatiles as attractive or repellent odors and aromas are of great interest in food and cosmetic bioprocesses, […]
Biofilm formation on medical devices is associated with hospital-acquired (nosocomial) infections. Due to biofilms’ high tolerance to antibiotics, classical treatments lack efficacy, and removal of the biofilm-contaminated device is often the only therapeutic option. […]
Genetic Methods and Tools
We originally developed genetic tools to study adhesion and biofilm formation in commensal and pathogenic E. coli species. These tools are made freely available to the scientific community upon request.• pKOBEG Lambda Red plasmidsBased […]
In vivo Study of Biofilm-Associated Catheter Infections
We developed a clinically relevant in vivo model of catheter-associated biofilm infections to design new and efficient biofilm-eradication approaches applicable to contaminated totally implantable venous access port (TIVAP) catheters. Key publication• Chauhan, A., D. Lebeaux, B. Decante, I. Kriegel, M.C. […]
We designed a continuous-flow culture system in which biofilm formation can be reproducibly monitored and controlled in microfermentors handled at flow-rates avoiding accumulation of planktonic bacteria. This model is suited for biofilm macroscopic analysis, […]
2017Comparative Analysis of Bacterial Community Composition and Structure in Clinically Symptomatic and Asymptomatic Central Venous Catheter., mSphere, in press.
2017Biofilm microenvironment induces a widespread adaptive amino-acid fermentation pathway conferring strong fitness advantage in Escherichia coli, PLoS Genetics, In Press.
2017Biological and chemical strategies for exploring inter- and intrakingdom communication mediated via bacterial volatile signals., Nat. Protoc. in press.
2016Functional analysis of Escherichia coli Yad fimbriae reveals their potential role in environmental persistence, Environ Microbiol. 2016 18(12);5228-5248.
2016Ultimate single-copy DNA detection using real-time electrochemical LAMP, ACS Sensors 1(7); 904-912.
2016Biofilms 2015: Multi-disciplinary approaches shed light into microbial life on surfaces, J Bacteriol. 2016 Mar 14. pii: JB.00156-16. [Epub ahead of print].
2016Study of in vivo catheter biofilm infections using pediatric central venous catheter implanted in rat, Nature Protocol 2016 Mar;11(3):525-41Epub 2016 Feb 18.
2015Airborne bacterial interactions: Functions out of thin air?, Front. Microbiol. Dec. 2015;6:1476..
2015In memoriam: A tribute to Cecile Wandersman, Volume: 166 Issue: 5 Pages: 393-398.
2015Selective propensity of bovine jugular vein material to bacterial adhesions: An in-vitro study., Int J Cardiol. 2015 Nov. 1;198:201-205. [Epub ahead of print Jul. 6].
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