Odile Sismeiro is holding a “Diplôme Universitaire de Technologie” and a “Diplôme d’Etudes Scientifiques et Techniques” (Conservatoire des Arts et Métiers, CNAM, Paris). She joined the Institut Pasteur in 1987 and worked for 13 years on the regulation of genetic expression in bacteria in Antoine Danchin’s research unit. She then joined the PF2 platform. She firstly contributed to the development of the DNA array technology at the platform and, in 2009, she established Next Generation Sequencing. Since then, as engineer, she conducted many projects aiming at gene expression profiling, from the quality control of the nucleic acids to the generation of sequencing data. She also has important user training and technological development activities. She is also taking a very active part in communication.
Workshop: Bacterial RNAseq
We propose a skills training for PhD students on Bacterial RNAseq experiments Using total RNAs, we will i) enrich the mRNAs by depleting the rRNAs ; ii) prepare and sequence cDNA library and iii) analyse […]
Training: analysis of the transcriptome by RNAseq
The Transcriptome and Epigenome platform of the Biomics pole is organizing a one week (October 10, 2016 – October 14, 2016) training session dedicated to the analysis of the transcriptome by RNAseq. It is […]
Deciphering the virulence factors of Mycobacterium abscessus activated in the presence of environmental amoebae
Mycobacterium abscessus (M. abs) is an emerging pathogen causing lung infections in cystic fibrosis patients. Although the environmental reservoir of M. abs is currently unknown, M. abs possesses a gene pool dedicated to a […]
The role of lncRNA H19 in Thymopoiesis
“The thymus is a specialized organ that provides a unique environment for T cell development and differentiation. The study the thymus-settling progenitors (TSPs) and how they differentiate is important for the characterization of the […]
Characterization of the SigmaS network by RNA sequencing and identification of SigmaS-controlled small regulatory RNAs in Salmonella
We have used directional RNA-sequencing and complementary assays to explore the SigmaS-dependent transcriptome of Salmonella enterica serovar Typhimurium. This study confirms the large regulatory scope of SigmaS and provides insights into its physiological functions […]
Escherichia coli transcriptomic analysis upon OmrA or OmrB overexpression
OmrA and OmrB are two small regulatory RNAs (sRNAs) conserved in enterobacteria. Their transcription is activated by the EnvZ-OmpR two-component system and their levels therefore increase in response to an increase in osmolarity. They […]
Intracellular membrane proliferation in E. coli
Overproduction of membrane proteins in the E. coli strains generally induce cell death. The multiple mutant C43 (DE3) derivative of BL21 (DE3) strain was selected to resist this overproduction. In this mutant, the membrane […]
HIV signature on RIG-I-like receptors
The pathogenesis of HIV infection, and in particular the development of immunodeficiency, remains incompletely understood. During HIV infection both innate and adaptive immune responses are raised, but they appear to be insufficient or too […]
Biostatistics for data analysis
The platform provides statistical analyses of RNA-seq count data. Before any project starts, we help the user to design his experiment in order to ensure that it really addresses the biological question he wishes […]
2019Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages, PLoS Pathog. 2019 Nov;15(11):e1008069.
2019Non-specific interference of cobalt with siderophore-dependent iron uptake pathways, Metallomics 2019 Oct;.
2019Transcriptional profiling of a laboratory and clinical Mycobacterium tuberculosis strain suggests respiratory poisoning upon exposure to delamanid, Tuberculosis (Edinb) 2019 Jul;117:18-23.
2018Enzymatic synthesis of random sequences of RNA and RNA analogues by DNA polymerase theta mutants for the generation of aptamer libraries, Nucleic Acids Res. 2018 07;46(12):6271-6284.
2018Enteric bacteria boost defences against oxidative stress in Entamoeba histolytica, Sci Rep 2018 Jun;8(1):9042.
2018Expansion of the SOS regulon of Vibrio cholerae through extensive transcriptome analysis and experimental validation, BMC Genomics 2018 May;19(1):373.
2017An ApiAP2 member regulates expression of clonally variant genes of the human malaria parasite Plasmodium falciparum, Sci Rep 2017 Oct;7(1):14042.
2017An Optimized Method for Extracting Bacterial RNA from Mouse Skin Tissue Colonized by Mycobacterium ulcerans, Front Microbiol 2017;8:512.
2017The Legionella pneumophila genome evolved to accommodate multiple regulatory mechanisms controlled by the CsrA-system, PLoS Genet. 2017 Feb;13(2):e1006629.
2017Genome-Wide Transcriptional Start Site Mapping and sRNA Identification in the Pathogen Leptospira interrogans, Front Cell Infect Microbiol 2017;7:10.
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