Timeline

Laurence Mulard

Head of Structure

20 Nov 2023

publication

Expedient synthesis of l-heptose derived septacidin building blocks from l-glucose

27 Apr 2022

publication

The First-in-Human Synthetic Glycan-Based Conjugate Vaccine Candidate against Shigella.

07 Mar 2022

publication

Toward a Multivalent Synthetic Oligosaccharide-Based Conjugate Vaccine against Shigella: State-of-the-Art for a Monovalent Prototype and Challenges.

01 Feb 2022

publication

Combination of High-Resolution Multistage Ion Mobility and Tandem MS with High Energy of Activation to Resolve the Structure of Complex Chemoenzymatically Synthesized Glycans.

13 Oct 2021

publication

Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides.

15 Jul 2021

publication

Multigram synthesis of an orthogonally-protected pentasaccharide for use as a glycan precursor in a Shigella flexneri 3a conjugate vaccine: application to a ready-for-conjugation decasaccharide

01 Apr 2021

publication

Safety and immunogenicity of a synthetic carbohydrate conjugate vaccine against Shigella flexneri 2a in healthy adult volunteers: a phase 1, dose-escalating, single-blind, randomised, placebo-controlled study.

26 Mar 2021

publication

Exploratory N-Protecting Group Manipulation for the Total Synthesis of Zwitterionic Shigella sonnei Oligosaccharides.

05 Feb 2021

publication

Convergent Chemoenzymatic Strategy to Deliver a Diversity of Shigella flexneri Serotype-Specific O-Antigen Segments from a Unique Lightly Protected Tetrasaccharide Core.

28 Jan 2021

publication

Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.

01 Jan 2019

publication

Classical and novel strategies to develop a Shigella glycoconjugate vaccine: from concept to efficacy in human.

01 Dec 2018

publication

ADP-heptose is a newly identified pathogen-associated molecular pattern of Shigella flexneri.

25 Aug 2018

project

GlycoShig1

25 Aug 2018

patent

Protected tetrasaccharides, their preparation and their use in the synthesis of bacterial oligosaccharides

25 Aug 2018

patent

Glycoconjugates and their use as potential vaccines against infection by Shigella flexneri (SF6)

25 Aug 2018

patent

Glycoconjugates and use thereof as vaccines against Shigella flexneri serotype 3a and X

25 Aug 2018

patent

Glycoconjugates and their use as potential vaccines against infection by Shigella flexneri

15 Aug 2018

publication

Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens.

24 Mar 2017

publication

Dirhamnolipids secreted from Pseudomonas aeruginosa modify anjpegungal susceptibility of Aspergillus fumigatus by inhibiting β1,3 glucan synthase activity.

04 Jul 2016

publication

Detailed Investigation of the Immunodominant Role of O-Antigen Stoichiometric O-Acetylation as Revealed by Chemical Synthesis, Immunochemistry, Solution Conformation and STD-NMR Spectroscopy for Shigella flexneri 3a

01 Jul 2016

publication

Efficient Iterative Synthesis of O-Acetylated Tri- to Pentadecasaccharides Related to the Lipopolysaccharide of Shigella flexneri Type 3 a through Di- and Trisaccharide Glycosyl Donors.

07 Jun 2016

team

Group: Carbohydrates and Bioconjugates

27 Mar 2016

project

A synthetic carbohydrate-based conjugate vaccine ready for a clinical trial

27 Mar 2016

project

FlexBiVac

21 Mar 2016

publication

A Synthetic Carbohydrate Conjugate Vaccine Candidate against Shigellosis: Improved Bioconjugation and Impact of Alum on Immunogenicity

12 Sep 2015

project

CarbUniVax

04 Sep 2015

publication

Investigation on the synthesis of Shigella flexneri-specific oligosaccharides using disaccharides as potential transglucosylase acceptor substrates

08 Jul 2015

project

Synthesis of peptide and protein conjugates towards novel therapeutics and/or diagnostic tools

03 Jul 2015

project

Carbohydrates as targets of immunity: their synthesis, antibody recognition and use as key vaccine components

11 May 2015

team

Chemistry of Biomolecules

14 Feb 2015

publication

Programmed chemo-enzymatic synthesis of the oligosaccharide component of a carbohydrate-based antibacterial vaccine candidate

07 Jan 2015

publication

Computer-aided design of sequence libraries for the engineering of transglucosylase binding subsites to enable glucosylation of an unnatural disaccharide motif of relevance for bacterial antigen synthesis

11 Sep 2014

publication

Synthesis of the zwitterionic repeating unit of the O-antigen from Shigella sonnei and chain elongation at both ends

21 Aug 2014

publication

Linear synthesis of the branched pentasaccharide repeats of O-antigens from Shigella flexneri 1a and 1b demonstrating the major steric hindrance associated with type-specific glucosylation

28 Jun 2014

publication

Non-stoichiometric O-acetylation of Shigella flexneri 2a O-specific polysaccharide: synthesis and antigenicity

20 Dec 2013

publication

CGTase-catalysed cis-glucosylation of L-rhamnosides for the preparation of Shigella flexneri 2a and 3a haptens

11 Oct 2013

publication

Concise synthesis of di- and trisaccharides related to the O-antigens from Shigella flexneri serotypes 6 and 6a, based on late stage mono-O-acetylation and/or site-selective oxydation

08 May 2013

publication

Structural studies of the O-acetyl containing O-antigen from a Shigella flexneri serotype 6 strain and synthesis of oligosaccharide fragments thereof

30 Oct 2012

publication

Applying pairwise combinations of amino acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides

16 Mar 2012

publication

Design of a specific colonic mucus marker using a human commensal bacterium cell surface domain

01 Jan 2012

publication

Multidisciplinary approaches to study O-antigen:antibody recognition in support of the development of synthetic carbohydrate-based enteric vaccines

24 May 2011

publication

Dynamic aspects of antibody:oligosaccharide complexes characterized by molecular dynamics simulations and saturation transfer difference nuclear magnetic resonance

28 Oct 2010

publication

Effects of backbone substitutions on the conformational behavior of Shigella flexneri O-antigens: implications for vaccine strategy

22 Jul 2010

publication

A pH-based high-throughput screening of sucrose-utilizing transglucosidases for the development of novel enzymatic glucosylation tools

23 Jul 2009

publication

From epitope characterization to the design of semi-synthetic glycoconjugate vaccines against Shigella flexneri 2a infection

24 Jun 2009

publication

Rational design and immunogenicity of liposome-based diepitope constructs: application to synthetic oligosaccharides mimicking the Shigella flexneri 2a O-antigen

01 Jun 2009

publication

Design of alpha-transglucosidases of controlled specificity for programmed chemoenzymatic synthesis of antigenic oligosaccharides

01 Apr 2009

publication

Efficient synthesis of six tri- to hexasaccharide fragments of Shigella flexneri serotypes 3a and/or X O-antigen, including a study on acceptors containing N-trichloroacetylglucosamine versus N-acetylglucosamine

27 Mar 2009

publication

Structural mimicry of O-antigen by a peptide revealed in a complex with an antibody raised against Shigella flexneri serotype 2a

01 Mar 2009

publication

Synthesis of L-rhamnose and N-acetyl-D-glucosamine derivatives with glucansucrases

15 Feb 2009

publication

A synthetic carbohydrate-protein conjugate vaccine candidate against Shigella flexneri 2a infection

13 Oct 2008

publication

Synthesis of 2 tetra- and 4 pentasaccharide fragments of Shigella flexneri serotypes 3a and/or X O-antigens from a common tetrasaccharide intermediate

03 Sep 2008

publication

Synthesis of branched tri- to pentasaccharides representative of fragments of Shigella flexneri serotypes 3a and/or X O-antigens

23 Jul 2008

publication

Investigation towards bivalent chemically defined glycoconjugate immunogens prepared from acid-detoxified lipopolysaccharide of Vibrio cholerae O1, serotype Inaba

10 Jul 2008

publication

Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody

10 Jul 2008

publication

Vaccination against shigellosis: is it the path that is difficult or is it the difficult that is the path?

15 Jun 2008

publication

Efficient route to orthogonally protected precursors of 2-acylamino-2-deoxy-3-O-substituted--D-glucopyranose derivatives and use thereof

01 Apr 2008

publication

Acyclic phosphonate nucleotides and human adenylate kinases: impact of a borano group on alpha-P position

01 Jan 2007

publication

[Carbohydrates and vaccines: from purified polysaccharides to semi-synthetic glycoconjugate vaccines]

01 Feb 2006

publication

Characterization of functional oligosaccharide mimics of the Shigella flexneri serotype 2a O-antigen: implications for the development of a chemically defined glycoconjugate vaccine

26 Oct 2005

publication

Toward a better understanding of the basis of the molecular mimicry of polysaccharide antigens by peptides: the example of Shigella flexneri 5a

01 Sep 2005

publication

On the preparation of carbohydrate-protein conjugates using the traceless Staudinger ligation

19 Apr 2005

publication

Suppression of multidrug-resistant HIV-1 reverse transcriptase primer unblocking activity by alpha-phosphate-modified thymidine analogues

18 Feb 2005

publication

Synthesis of two linear PADRE conjugates bearing a deca- or pentadecasaccharide B epitope as potential synthetic vaccines against Shigella flexneri serotype 2a infection

01 Jan 2005

publication

Borano-nucleotides: new analogues to circumvent HIV-1 RT-mediated nucleoside drug-resistance

17 Nov 2004

publication

Mechanistic insights into the suppression of drug resistance by human immunodeficiency virus type 1 reverse transcriptase using alpha-boranophosphate nucleoside analogs

20 Jun 2004

publication

Total synthesis of a tetra- and two pentasaccharide fragments of the O-specific polysaccharide of Shigella flexneri serotype 2a

19 May 2004

publication

A structural basis for the inhibition of the NS5 dengue virus mRNA 2′-O-methyltransferase domain by ribavirin 5′-triphosphate

26 Apr 2004

publication

Preparation of synthetic glycoconjugates as potential vaccines against Shigella flexneri serotype 2a disease

20 Feb 2004

publication

Blockwise approach to fragments of the O-specific polysaccharide of Shigella flexneri serotype 2a: convergent Synthesis of a decasaccharide representative of a dimer of the branched repeating unit

18 Aug 2003

publication

Conformational studies of the O-specific polysaccharide of Shigella flexneri 5a and of four related synthetic pentasaccharide fragments using NMR and molecular modeling

01 Mar 2003

publication

Structural analysis of the activation of ribavirin analogs by NDP kinase: comparison with other ribavirin targets

16 Oct 2002

publication

Synthesis of the methyl glycoside of a branched octasaccharide fragment specific for the Shigella flexneri serotype 2a O-antigen

08 Aug 2002

publication

Improving nucleoside diphosphate kinase for antiviral nucleotide analogs activation

09 Jul 2002

publication

Convergent synthesis, NMR and conformational analysis of tetra- and pentasaccharide haptens of the Shigella flexneri 5a O-specific polysaccharide

19 Feb 2002

publication

Synthesis and NMR study of a linear pentasaccharide fragment of the Shigella flexneri 5a O-specific polysaccharide

01 Jan 2002

publication

Vaccins polysaccharidiques

01 Nov 2001

publication

Conformation of the O-specific polysaccharide of Shigella dysenteriae type 1: molecular modeling shows a helical structure with efficient exposure of the antigenic determinant alpha-L-Rhap-(1–>2)-alpha-D-Galp

08 Aug 2001

publication

MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis

01 Apr 2001

publication

Activation of anti-reverse transcriptase nucleotide analogs by nucleoside diphosphate kinase: improvement by alpha-boranophosphate substitution

27 Dec 2000

publication

The valine-to-threonine 75 substitution in human immunodeficiency virus type 1 reverse transcriptase and its relation with stavudine resistance

27 Sep 2000

publication

Linear synthesis of the methyl glycosides of tetra- and pentasaccharide fragments specific for the Shigella flexneri serotype 2a O-antigen

01 Jan 2000

publication

Linear synthesis of the methyl glycosides of tri-, tetra-, and pentasaccharide fragments of the Shigella flexneri serotype 5a O-antigen

01 Jan 2000

publication

Synthesis of the methyl glycosides of a di- and two trisaccharide fragments specific for the Shigella flexneri serotype 2a O-antigen

01 Jan 2000

publication

First synthesis of a branched pentasaccharide representative of the repeating unit of the Shigella flexneri serotype 5a O-antigen

01 Sep 1999

publication

Immunochemical characterization of an Ogawa-Inaba common antigenic determinant of Vibrio cholerae O1

01 Jan 1999

publication

Synthesis of the methyl glycosides of a tri- and tetrasaccharide fragment specific for the Shigella flexneri serotype 5a O-antigen. A reinvestigation

01 Sep 1998

publication

Synthesis of tri- and tetrasaccharide fragments of the Shigella dysenteriae type 1 O-antigen deoxygenated and fluorinated at position 3 of the methyl alpha-D-galactopyranoside terminus

01 Jul 1998

publication

Binding of modified fragments of the Shigella dysenteriae type 1 O-specific polysaccharide to monoclonal IgM 3707 E9 and docking of the immunodeterminant to its modeled Fv

01 Jan 1997

publication

Investigation by NMR spectroscopy and molecular modeling of the conformations of some modified disaccharide antigens for Shigella dysenteriae type 1

01 Sep 1995

publication

Synthesis of specifically deoxygenated disaccharide derivatives of the Shigella dysenteriae type 1 O-antigen

01 Jun 1994

publication

Synthesis of specifically monofluorinated ligands related to the O-polysaccharide of Shigella dysenteriae type 1

01 Jun 1994

publication

Fluorination at position 6 of derivatives of methyl alpha-D-galactopyranoside

01 Jan 1994

publication

Synthesis of methyl O-alpha-L-rhamnopyranosyl-(1–>2)-alpha-D-galactopyranosides specifically deoxygenated at position 3, 4, or 6 of the galactose residue

01 Jan 1993

publication

A little guide for advanced search:

Expedient synthesis of l-heptose derived septacidin building blocks from l-glucose

The First-in-Human Synthetic Glycan-Based Conjugate Vaccine Candidate against Shigella.

Toward a Multivalent Synthetic Oligosaccharide-Based Conjugate Vaccine against Shigella: State-of-the-Art for a Monovalent Prototype and Challenges.

Combination of High-Resolution Multistage Ion Mobility and Tandem MS with High Energy of Activation to Resolve the Structure of Complex Chemoenzymatically Synthesized Glycans.

Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides.

Multigram synthesis of an orthogonally-protected pentasaccharide for use as a glycan precursor in a Shigella flexneri 3a conjugate vaccine: application to a ready-for-conjugation decasaccharide

Safety and immunogenicity of a synthetic carbohydrate conjugate vaccine against Shigella flexneri 2a in healthy adult volunteers: a phase 1, dose-escalating, single-blind, randomised, placebo-controlled study.

Exploratory N-Protecting Group Manipulation for the Total Synthesis of Zwitterionic Shigella sonnei Oligosaccharides.

Convergent Chemoenzymatic Strategy to Deliver a Diversity of Shigella flexneri Serotype-Specific O-Antigen Segments from a Unique Lightly Protected Tetrasaccharide Core.

Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.

Classical and novel strategies to develop a Shigella glycoconjugate vaccine: from concept to efficacy in human.

ADP-heptose is a newly identified pathogen-associated molecular pattern of Shigella flexneri.

GlycoShig1

Protected tetrasaccharides, their preparation and their use in the synthesis of bacterial oligosaccharides

Glycoconjugates and their use as potential vaccines against infection by Shigella flexneri (SF6)

Glycoconjugates and use thereof as vaccines against Shigella flexneri serotype 3a and X

Glycoconjugates and their use as potential vaccines against infection by Shigella flexneri

Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens.

Dirhamnolipids secreted from Pseudomonas aeruginosa modify anjpegungal susceptibility of Aspergillus fumigatus by inhibiting β1,3 glucan synthase activity.

Detailed Investigation of the Immunodominant Role of O-Antigen Stoichiometric O-Acetylation as Revealed by Chemical Synthesis, Immunochemistry, Solution Conformation and STD-NMR Spectroscopy for Shigella flexneri 3a

Efficient Iterative Synthesis of O-Acetylated Tri- to Pentadecasaccharides Related to the Lipopolysaccharide of Shigella flexneri Type 3 a through Di- and Trisaccharide Glycosyl Donors.

Group: Carbohydrates and Bioconjugates

A synthetic carbohydrate-based conjugate vaccine ready for a clinical trial

FlexBiVac

A Synthetic Carbohydrate Conjugate Vaccine Candidate against Shigellosis: Improved Bioconjugation and Impact of Alum on Immunogenicity

CarbUniVax

Investigation on the synthesis of Shigella flexneri-specific oligosaccharides using disaccharides as potential transglucosylase acceptor substrates

Synthesis of peptide and protein conjugates towards novel therapeutics and/or diagnostic tools

Carbohydrates as targets of immunity: their synthesis, antibody recognition and use as key vaccine components

Chemistry of Biomolecules

Programmed chemo-enzymatic synthesis of the oligosaccharide component of a carbohydrate-based antibacterial vaccine candidate

Computer-aided design of sequence libraries for the engineering of transglucosylase binding subsites to enable glucosylation of an unnatural disaccharide motif of relevance for bacterial antigen synthesis

Synthesis of the zwitterionic repeating unit of the O-antigen from Shigella sonnei and chain elongation at both ends

Linear synthesis of the branched pentasaccharide repeats of O-antigens from Shigella flexneri 1a and 1b demonstrating the major steric hindrance associated with type-specific glucosylation

Non-stoichiometric O-acetylation of Shigella flexneri 2a O-specific polysaccharide: synthesis and antigenicity

CGTase-catalysed cis-glucosylation of L-rhamnosides for the preparation of Shigella flexneri 2a and 3a haptens

Concise synthesis of di- and trisaccharides related to the O-antigens from Shigella flexneri serotypes 6 and 6a, based on late stage mono-O-acetylation and/or site-selective oxydation

Structural studies of the O-acetyl containing O-antigen from a Shigella flexneri serotype 6 strain and synthesis of oligosaccharide fragments thereof

Applying pairwise combinations of amino acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides

Design of a specific colonic mucus marker using a human commensal bacterium cell surface domain

Multidisciplinary approaches to study O-antigen:antibody recognition in support of the development of synthetic carbohydrate-based enteric vaccines

Dynamic aspects of antibody:oligosaccharide complexes characterized by molecular dynamics simulations and saturation transfer difference nuclear magnetic resonance

Effects of backbone substitutions on the conformational behavior of Shigella flexneri O-antigens: implications for vaccine strategy

A pH-based high-throughput screening of sucrose-utilizing transglucosidases for the development of novel enzymatic glucosylation tools

From epitope characterization to the design of semi-synthetic glycoconjugate vaccines against Shigella flexneri 2a infection

Rational design and immunogenicity of liposome-based diepitope constructs: application to synthetic oligosaccharides mimicking the Shigella flexneri 2a O-antigen

Design of alpha-transglucosidases of controlled specificity for programmed chemoenzymatic synthesis of antigenic oligosaccharides

Efficient synthesis of six tri- to hexasaccharide fragments of Shigella flexneri serotypes 3a and/or X O-antigen, including a study on acceptors containing N-trichloroacetylglucosamine versus N-acetylglucosamine

Structural mimicry of O-antigen by a peptide revealed in a complex with an antibody raised against Shigella flexneri serotype 2a

Synthesis of L-rhamnose and N-acetyl-D-glucosamine derivatives with glucansucrases

A synthetic carbohydrate-protein conjugate vaccine candidate against Shigella flexneri 2a infection

Synthesis of 2 tetra- and 4 pentasaccharide fragments of Shigella flexneri serotypes 3a and/or X O-antigens from a common tetrasaccharide intermediate

Synthesis of branched tri- to pentasaccharides representative of fragments of Shigella flexneri serotypes 3a and/or X O-antigens

Investigation towards bivalent chemically defined glycoconjugate immunogens prepared from acid-detoxified lipopolysaccharide of Vibrio cholerae O1, serotype Inaba

Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody

Vaccination against shigellosis: is it the path that is difficult or is it the difficult that is the path?

Efficient route to orthogonally protected precursors of 2-acylamino-2-deoxy-3-O-substituted--D-glucopyranose derivatives and use thereof

Acyclic phosphonate nucleotides and human adenylate kinases: impact of a borano group on alpha-P position

[Carbohydrates and vaccines: from purified polysaccharides to semi-synthetic glycoconjugate vaccines]

Characterization of functional oligosaccharide mimics of the Shigella flexneri serotype 2a O-antigen: implications for the development of a chemically defined glycoconjugate vaccine

Toward a better understanding of the basis of the molecular mimicry of polysaccharide antigens by peptides: the example of Shigella flexneri 5a

On the preparation of carbohydrate-protein conjugates using the traceless Staudinger ligation

Suppression of multidrug-resistant HIV-1 reverse transcriptase primer unblocking activity by alpha-phosphate-modified thymidine analogues

Synthesis of two linear PADRE conjugates bearing a deca- or pentadecasaccharide B epitope as potential synthetic vaccines against Shigella flexneri serotype 2a infection

Borano-nucleotides: new analogues to circumvent HIV-1 RT-mediated nucleoside drug-resistance

Mechanistic insights into the suppression of drug resistance by human immunodeficiency virus type 1 reverse transcriptase using alpha-boranophosphate nucleoside analogs

Total synthesis of a tetra- and two pentasaccharide fragments of the O-specific polysaccharide of Shigella flexneri serotype 2a

A structural basis for the inhibition of the NS5 dengue virus mRNA 2′-O-methyltransferase domain by ribavirin 5′-triphosphate

Preparation of synthetic glycoconjugates as potential vaccines against Shigella flexneri serotype 2a disease

Blockwise approach to fragments of the O-specific polysaccharide of Shigella flexneri serotype 2a: convergent Synthesis of a decasaccharide representative of a dimer of the branched repeating unit

Conformational studies of the O-specific polysaccharide of Shigella flexneri 5a and of four related synthetic pentasaccharide fragments using NMR and molecular modeling

Structural analysis of the activation of ribavirin analogs by NDP kinase: comparison with other ribavirin targets

Synthesis of the methyl glycoside of a branched octasaccharide fragment specific for the Shigella flexneri serotype 2a O-antigen

Improving nucleoside diphosphate kinase for antiviral nucleotide analogs activation

Convergent synthesis, NMR and conformational analysis of tetra- and pentasaccharide haptens of the Shigella flexneri 5a O-specific polysaccharide

Synthesis and NMR study of a linear pentasaccharide fragment of the Shigella flexneri 5a O-specific polysaccharide

Vaccins polysaccharidiques

Conformation of the O-specific polysaccharide of Shigella dysenteriae type 1: molecular modeling shows a helical structure with efficient exposure of the antigenic determinant alpha-L-Rhap-(1–>2)-alpha-D-Galp

MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis