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© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : Organic letters

Synthesis of the zwitterionic repeating unit of the O-antigen from Shigella sonnei and chain elongation at both ends

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Organic letters - 11 Sep 2014

Pfister HB, Mulard LA

Link to Pubmed [PMID] – 25210812

Org. Lett. 2014 Sep;16(18):4892-5

Shigella sonnei O-antigen features a zwitterionic disaccharide repeat encompassing two rare monosaccharides. The synthesis of the AB repeat and of trisaccharides ABA’ and B’AB, which validates chain elongation at either end, is reported. All targets were synthesized using a postglycosylation oxidation strategy in combination with imidate chemistry. Precursors to residue A were obtained from L-glucose. The AAT (B) donor and acceptor were obtained from D-glucosamine. A one-step Pd(OH)2/C-mediated deprotection provided the propyl glycoside targets.

http://www.ncbi.nlm.nih.gov/pubmed/25210812