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© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : Nucleosides, nucleotides & nucleic acids

Activation of anti-reverse transcriptase nucleotide analogs by nucleoside diphosphate kinase: improvement by alpha-boranophosphate substitution

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleosides, nucleotides & nucleic acids - 01 Apr 2001

Schneider B, Meyer P, Sarfati S, Mulard L, Guerreiro C, Boretto J, Janin J, Véron M, Deville-Bonne D, Canard B

Link to Pubmed [PMID] – 11563041

Nucleosides Nucleotides Nucleic Acids 2001 Apr-Jul;20(4-7):297-306

Nucleoside activation by nucleoside diphosphate kinase and inhibition of HIV-1 reverse transcriptase were studied comparatively for a new class of nucleoside analogs with a borano (BH3-) or a thio (SH) group on the alpha-phosphate. Both the alpha-Rp-borano derivatives of AZT and d4T improved phosphorylation by NDP kinase, inhibition of reverse transcription as well as stability of alpha-borano nonophosphate derivatives in terminated viral DNA chain.

http://www.ncbi.nlm.nih.gov/pubmed/11563041