Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share
© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : Journal of Carbohydrate Chemistry

Synthesis of L-rhamnose and N-acetyl-D-glucosamine derivatives with glucansucrases

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of Carbohydrate Chemistry - 01 Mar 2009

Champion C, André I, Mulard LA, Monsan P, Remaud-Siméon M, Morel S

J. Carbohydr. Chem. 2009; 28: 142-60

Transglucosylation reactions using sucrose as glucosyl donor and either N-acetyl-Dglucosamine, L-rhamnose, or methyl α-L-rhamnopyranoside as acceptors were carried out with recombinant glucansucrases from families 70 and 13 of glycosidehydrolases. Depending on the enzyme specificity, various carbohydrate structures were synthesized and characterized including α-D-glucopyranosyl-(1→6)-N-acetyl-Dglucosamine, α-D-glucopyranosyl-(1→4)-N-acetyl-D-glucosamine, α-D-glucopyranosyl- (1→1)-β-L-rhamnopyranoside, α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→1)-β-L-rhamnopyranoside, methyl α-D-glucopyranosyl-(1→4)-α-L-rhamnopyranoside, and methyl α-D-glucopyranosyl-(1→3)-α-L-rhamnopyranoside. Disaccharides were obtained with yields going up to 64%. The structural diversity generated as well as the obtained yields appear to be related to enzyme active site architecture, which can be modulated and improved by enzyme engineering. Several of the obtained disaccharides enter in the composition of surface polysaccharides of pathogenic bacteria, among which is Shigella flexneri. Our results outline the potential of glucansucrases in the chemoenzymatic synthesis of complex carbohydrates of biological interest whose chemical synthesis may be seen as a limitation.