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© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : Tetrahedron

Total synthesis of a tetra- and two pentasaccharide fragments of the O-specific polysaccharide of Shigella flexneri serotype 2a

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Tetrahedron - 20 Jun 2004

Mulard LA, Guerreiro C

Tetrahedron 2004; 60: 2475-88

The synthesis of the methyl glycoside of the branched pentasaccharide biological repeating unit of the O-antigen of Shigella flexneri serotype 2a is described together with that of the methyl glycoside of the corresponding tetrasaccharide and frame-shifted linear pentasaccharide. All the strategies disclosed herein involve a key disaccharide corresponding to the branching point and otherwise appropriate monosaccharide building blocks activated as their trichloroacetimidate. Our data suggest partial lack of conformational
flexibility at the branched residue.