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© Valérie Choumet
Mosquitoes were orally infected with the chikungunya virus. Midguts were dissected at day 5 post-infection, fixed and permeabilised. Virus is shown in red (anti-E2 protein, cyanine 3), the actin network in green (phalloidin 548) and nuclei in blue (DAPI).
Publication : The Journal of organic chemistry

On the preparation of carbohydrate-protein conjugates using the traceless Staudinger ligation

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of organic chemistry - 01 Sep 2005

Grandjean C, Boutonnier A, Guerreiro C, Fournier JM, Mulard LA

Link to Pubmed [PMID] – 16122231

J. Org. Chem. 2005 Sep;70(18):7123-32

[reaction: see text] The nature of a linker used for preparing glycoconjugate vaccines is of utmost importance as it may lead to immunogenic biomolecules. We report the conjugation of carbohydrate haptens to protein carriers leading to potential vaccines using the traceless Staudinger ligation. The ligation relies on the selective transfer of a phosphane substituent to an azide to form a native amide bond in the final product upon release of an oxidized phosphane byproduct. We designed new phosphino-functionalized cross-linkers suitable for protein carrier derivatization. We evaluated their utility in preparing conjugates using both synthetic and purified bacterial carbohydrates. The use of a borane-protected phosphane which is deprotected at the time of the ligation reaction led to the best results observed thus far in terms of stability toward oxidation and reactivity.

http://www.ncbi.nlm.nih.gov/pubmed/16122231