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  • Permanent Researcher
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  • PhD Student
  • Physician
  • Post-doc
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  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
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  • Director of Center
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About

The Mass Spectrometry for Biology Laboratory is  a mixed Institut Pasteur/CNRS Unit  (USR 2000) and is headed by Julia Chamot-Rooke (DR1 CNRS). The lab includes the Pasteur proteomics platform and a research group, dedicated to the development of top-down proteomics and structural mass spectrometry. The laboratory is  part of the C2RT (Center or Technological Research and Ressources) and of the Structural Biology and Chemistry department. It is IBISA labeled since 2012 and ISO 9001:2015 certified since April 2018.

The platform, headed by M. Matondo, provides service and collaboration in bottom-up proteomics. It is open to external users.

The research group develops four main axes:
Top-down proteomics is an emerging technology based on the analysis of intact proteins using very high-resolution mass spectrometry. It provides the highest molecular precision for analyzing primary structures and determining post-translational modifications by examining proteins in their intact state, leading to more straightforward and reliable results than the classical bottom-up approach based on protein enzymatic digestion. Top-down proteomics holds great promise in clinical research and in particular in clinical microbiology, a major research axis of the unit.
Cross-linking combined to mass spectrometry is a powerful approach to address both the structure and composition of protein complexes. To improve the crucial stage of detection and characterization of cross-linked peptides, we have developed a new generation of cross-linking agents based on click-chemistry.
Native MS allows the structural investigation of protein complexes with the sensitivity, speed, unlimited mass range and accuracy of mass spectrometry. The exact mass of the proteins can be obtained, as well as the stoichiometry of the assembly, its conformation and topological arrangement of the subunits within the complex.
HDX (Hydrogen Deuterium Exchange) MS is based on the different exchange rates of hydrogens in proteins. Exchange rates are a function of solvent accessibility and hydrogen bonding. HDX MS can be used to monitor conformational changes in proteins, characterize protein folding pathways or protein-protein interactions. HDX MS is also widely used for epitope mapping.

For more information on these different topics, please contact Julia Chamot-Rooke

Equipments

Orbitrap Eclipse Tribrid Mass Spectrometer • Thermo Fisher • Orbitrap Eclipse™ Tribrid™ Mass Spectrometer

The Orbitrap Eclipse™ Tribrid™ mass spectrometer is cuppled witrh Neo Vanquish or an easy-nLC (Proxeon) and has been installed in January 2021. It is used for: – TMT quantification – Data Independent Acquisition (DIA) […]

Orbitrap Q-Exactive HF Mass Spectrometer • Thermo Fisher • Orbitrap Q Exactive™ HF

The Orbitrap Q-Exactive HF is equipped with an easy-nLC (Proxeon) and has been installed in 2014/2015. It is used for: Protein identification (Data Dependent and Data Independent Analyses) Post translational modification (PTM) analysis Quantitative […]

Orbitrap Fusion Lumos Mass Spectrometer • Thermo Fisher • Orbitrap Tribrid Fusion Lumos

An Orbitrap Fusion has been installed in 2013 and upgraded in Fusion Lumos in 2016. The instrument is equipped with ETD and used either with a nanoUPLC Dionex for online fragmentation or with a Nanomate for […]

Orbitrap Velos Pro Mass Spectrometer • Thermo Fisher • LTQ-Velos Orbitrap

The LTQ-Velos Orbitrap is equipped with a U3000 nanoLC (Dionex) and ETD. It is mainly used for the analysis of simple mixtures of proteins after trypsin digestion or PTM analysis using its ETD capabilities.

Orbitrap Q-Exactive Plus Mass Spectrometer • Thermo Fisher • Q-Exactive Plus

The Q-Exactive Plus is equipped with an easy-nLC (Proxeon) and has been installed in 2014/2015. It is used for: Protein identification (Data Dependent and Data Independent Analyses) Post translational modification (PTM) analysis Quantitative proteomics […]

Members

Former Members

2000
2000
Name
Position
2013
2015
Egor Vorontsov
Post-doc
2015
2020
Thomas Ottone
Undergraduate Student
2012
2017
Véronique Hourdel
Research Engineer
2013
2018
Sébastien Brier
Research Engineer
2018
2019
Helisa Wippel
PhD Student (Brazil)
2015
2019
Diogo Borges-Lima
Post-doc
2019
2019
Talyta Soares
Graduate Student (Brazil)
2012
2015
Catherine Nury
Post-doc
2012
2014
Joseph Gault
PhD Student (Ecole Polytechnique)
2019
2019
Fabiana San Martin
PhD Student (Uruguay)
2013
2022
Thibaut Chaze
Research Engineer
2021
2022
David Tabb
Scientist researcher
2020
2023
Jonathan Dhenin
PhD Student
2021
2023
Lucienne Nouchikian
PhD Student
2022
2023
Rita Azevedo
Research Engineer
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Projects

Transversal Project

Fundings

Publications

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Contact

Phone: +33 1 40 61 38 59 Email: julia.chamot-rooke@pasteur.fr Address 25-28 Rue du Docteur Roux 75015, Paris France