Les chercheurs, ingénieurs, étudiants et stagiaires post-doctoraux du département Biologie structurale et chimie mettent en œuvre un ensemble intégré d’approches pour l’étude des systèmes biologiques complexes. A travers l’observation et l’expérimentation en utilisant les outils technologiques les plus performants, nous sommes en mesure d’appréhender, à différentes échelles jusque dans leurs plus infimes détails, les mécanismes et la structure des molécules. D’une part, le département inclut une forte composante de biologie structurale, faisant notamment appel à la diffraction des rayons X, la résonance magnétique nucléaire et la cryo-microscopie électronique. Par ailleurs, la composante chimie du département se concentre en priorité sur les polysaccharides et les acides nucléiques. Plusieurs équipes étudient également les interactions et réactions moléculaires, et leur impact physiologique au niveau cellulaire, par des méthodes biochimiques, biophysiques et microscopiques. La simulation et la prédiction in silico vient compléter l’ensemble d’approches mises en œuvre au sein du Département. Toutes ces études bénéficient de l’équipement de pointe fédéré au sein d’un large ensemble de plates-formes technologiques. Le département comprend deux UMR CNRS : l’UMR 3528 « Biologie structurale des processus cellulaires et maladies infectieuses », et l’UMR 3523 « Unité de Chimie Organique (UCO) ».
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Connexions
Présentation
Équipes
Platform
Ingénierie des Anticorps
Pierre Lafaye
Laboratory
Biochimie des Interactions Macromoléculaires
Daniel Ladant
Laboratory
Chimie bioorganique des acides nucléiques
Marcel Hollenstein
Laboratory
Chimie des biomolécules
Laurence Mulard
Platform
Cristallographie
Ahmed Haouz
Platform
Biophysique moléculaire
Patrick England
Platform
Protéomique
Mariette Matondo
Laboratory
Bioinformatique structurale
Michael Nilges
Laboratory
Architecture et Dynamique des Macromolécules Biologiques
Marc Delarue
UTechS
Spectrométrie de Masse pour la Biologie (UTechS MSBio)
Julia Chamot-Rooke
Laboratory
Microbiologie structurale
Pedro Alzari
Laboratory
Chimie Biologique Epigénétique
Paola Arimondo
Platform
Nanoimagerie
Matthijn Vos
Platform
Plate-Forme Technologique de RMN Biologique et HDX-MS
Iñaki Guijarro
Platform
Plateforme de Criblage Chémogénomique et Biologique (PF-CCB)
Fabrice Agou
Platform
Plate-Forme Technologique Production et Purification de Protéines Recombinantes
Stéphane Petres
Laboratory
Systèmes transmembranaires bactériens
Nadia Izadi-Pruneyre
Laboratory
Biologie Structurale Computationnelle
Max Bonomi
Laboratory
Mécanismes du Cycle Cellulaire Bactérien
Anne Marie Wehenkel
Laboratory
Biologie Cellulaire Structurale Intégrative
Ariane Briegel
Équipes associées
Laboratory
Signalisation et dynamique des récepteurs
Pierre-Jean Corringer
Laboratory
G5
Biologie structurale des maladies infectieuses
Pablo Guardado-Calvo
Collection
Collection des Cyanobactéries
Muriel Gugger
Laboratory
Virologie structurale
Félix Rey
Gestion
Paola Arimondo
Responsable de Structure
Directeur
Anciens Responsables
Emplois
Sélection de Projets
Sélection de Publications
Télécharger-
2024High Content Screening Assay of Inhibitors of the Legionella pneumophila Histone Methyltransferase RomA in Infected Cells., Chembiochem 2024 Sep; (): e202400293.
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2023Structure and dynamic association of an assembly platform subcomplex of the bacterial type II secretion system., Structure 2023 Feb; 31(2): 152-165.e7.
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2022Morphological control enables nanometer-scale dissection of cell-cell signaling complexes., Nat Commun 2022 Dec; 13(1): 7831.
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2022FtsEX-independent control of RipA-mediated cell separation in Corynebacteriales., Proc Natl Acad Sci U S A 2022 Dec; 119(50): e2214599119.
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2022In-depth characterization of the Clostridioides difficile phosphoproteome to identify Ser/Thr kinase substrates, Mol Cell Proteomics .
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2022DNA methylome combined with chromosome cluster-oriented analysis provides an early signature for cutaneous melanoma aggressiveness., Elife 2022 Sep; 11(): .
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2022Evaluation of 3′-phosphate as a transient protecting group for controlled enzymatic synthesis of DNA and XNA oligonucleotides., Commun Chem 2022 Jun; 5(1): 68.
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2022Spindle-shaped archaeal viruses evolved from rod-shaped ancestors to package a larger genome, Cell 2022 Apr; 185(8): 1297-1307.e11.
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2022Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog, Commun Biol . 2022 Apr 13;5(1):355. .
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2022Discovery of bat coronaviruses close to SARS-CoV-2 and infectious for human cells., Nautre 604 (2022) 330.
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2022Atomic structure of Lanreotide nanotubes revealed by cryo-EM., Proc Natl Acad Sci .
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2021InDeep: 3D fully convolutional neural networks to assist in silico drug design on protein-protein interactions., Bioinformatics 2022 Feb; 38(5): 1261-1268.
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2021Actinobacteria challenge the paradigm: A unique protein architecture for a well-known, central metabolic complex., Proc Natl Acad Sci U S A 2021 Nov; 118(48): .
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2021Computational and biochemical analysis of type IV pilus dynamics and stability., Structure 2021 Dec; 29(12): 1397-1409.e6.
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2021Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA., Nat Commun 2021 08; 12(1): 4710.
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2021A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells., Adv Sci (Weinh) 2021 05; 8(9): 2003630.
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2021The actomyosin interface contains an evolutionary conserved core and an ancillary interface involved in specificity., Nat Commun 2021 03; 12(1): 1892.
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2021Advanced In Vivo Cross-Linking Mass Spectrometry Platform to Characterize Proteome-Wide Protein Interactions., Anal Chem 2021 Feb; (): .
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2021Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli., PLoS One 2021 ; 16(4): e0250610.
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2020Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker., Proc. Natl. Acad. Sci. U.S.A. 2020 Jul; (): .
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2020DNA Methylation Bisubstrate Inhibitors Are Fast-Acting Drugs Active against Artemisinin-Resistant Plasmodium falciparum Parasites., ACS Cent Sci 2020 Jan; 6(1): 16-21.
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2019Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche, Cell Reports, 2019, 29 (12), pp.3958-3973.e7. ⟨10.1016/j.celrep.2019.11.049⟩.
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2019High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture., Proc Natl Acad Sci U S A 2019 01; 116(4): 1267-1272.
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2019LGP2 binds to PACT to regulate RIG-I- and MDA5-mediated antiviral responses., Sci Signal 2019 Oct; 12(601): .
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2019Maximizing binary interactome mapping with a minimal number of assays, Nat Commun 2019 Aug;10(1):3907.
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2019Compatibility of 5-ethynyl-2’F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers, Org. Biomol. Chem. 2019, 17, 8083 - 8087.
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2019The Many Faces of Epigenetics, Oxford, December 2017, Epigenetics 2019 Apr 17:1-9.
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2019Crystal structure of the Trypanosoma cruzi EIF4E5 translation factor homologue in complex with mRNA cap-4, Nucleic Acids Res. 2019 Jun;47(11):5973-5987.
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2019The Biosynthesis of Flavin Cofactors in Listeria monocytogenes, M. Sebastián, S. Arilla-Luna, J. Bellalou, et al., The Biosynthesis of Flavin Cofactors in Listeria monocytogenes, Journal of Molecular Biology.
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2019Structural insights into the functional versatility of an FHA domain protein in mycobacterial signaling., Sci Signal 2019 05; 12(580): .
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2019Double autoinhibition mechanism of signal transduction ATPases with numerous domains (STAND) with a tetratricopeptide repeat sensor, Nucleic Acids Res. 2019 Apr;47(7):3795-3810.
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2019Orthobunyavirus spike architecture and recognition by neutralizing antibodies., Nat Commun 2019 Feb; 10(1): 879.
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2019Overall Structures of Mycobacterium tuberculosis DNA Gyrase Reveal the Role of a Corynebacteriales GyrB-Specific Insert in ATPase Activity, Structure 2019 04;27(4):579-589.e5.
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2019First-in-class allosteric inhibitors of bacterial IMPDHs, Eur J Med Chem 2019 Feb;167:124-132.
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2019Towards the enzymatic formation of artificial metal base pairs with a carboxy-imidazole-modified nucleotide, J. Inorg. Biochem. 2019, 191, 154-163.
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2018Target engagement and binding mode of an anti-tuberculosis drug to its bacterial target deciphered in whole living cells by NMR., Biochemistry. 2019 Feb 12;58(6):526-533. doi: 10.1021/acs.biochem.8b00975. .
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2018Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting, Nature Communications, 2018, 9 (1), ⟨10.1038/s41467-018-06948-x⟩.
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2018eXL-MS: An Enhanced Cross-Linking Mass Spectrometry Workflow To Study Protein Complexes., Anal Chem 2018 09; 90(18): 10707-10714.
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2018, Philos Trans R Soc Lond B Biol Sci 2018 06; 373(1748): .
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2018Infection Reveals a Modification of SIRT2 Critical for Chromatin Association., Cell Rep 2018 04; 23(4): 1124-1137.
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2018Crystal structures of a pentameric ion channel gated by alkaline pH show a widely open pore and identify a cavity for modulation, Proc. Natl. Acad. Sci. U.S.A. 2018 04;115(17):E3959-E3968.
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2017Facile immobilization of DNA using an enzymatic his-tag mimic, Chem. Commun., 2017, 53, 13031-13034.
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2017A glycerophospholipid-specific pocket in the RVFV class II fusion protein drives target membrane insertion., Science 2017 Nov; 358(6363): 663-667.
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2017Structure of the calcium-dependent type 2 secretion pseudopilus, Nat Microbiol. 2017 Oct 9. doi: 10.1038/s41564-017-0041-2.
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2017Cryo-EM structure of the bacteriophage T4 isometric head at 3.3-Å resolution and its relevance to the assembly of icosahedral viruses., PNAS 26, 2017 114 (39) E8184-E8193.
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2017Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring, Nat. Biotechnol. 2017 Oct;35(10):977-982.
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2017Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics., Sci Rep 2017 05; 7(1): 1347.
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2017Nano-scale actin-network characterization of fibroblast cells lacking functional Arp2/3 complex., J Struct Biol 2017 03; 197(3): 312-321.
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2017Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis., PLoS Biol 2017 Dec; 15(12): e2004486.
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2016Large-scale synthesis and structural analysis of a synthetic glycopeptide dendrimer as an anti-cancer vaccine candidate, Org. Biomol. Chem. 2016 Dec;15(1):114-123.
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2016Camelid single-domain antibodies: A versatile tool for in vivo imaging of extracellular and intracellular brain targets., J Control Release 2016 Dec; 243(): 1-10.
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2016Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins, Nat Commun 2016 Dec;7:13582.
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2016CD4-mimetic sulfopeptide conjugates display sub-nanomolar anti-HIV-1 activity and protect macaques against a SHIV162P3 vaginal challenge, Sci Rep 2016 Oct;6:34829.
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2016Mutations in actin used for structural studies partially disrupt β-thymosin/WH2 domains interaction, FEBS Lett. 2016 Sep;.
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2016Shared active site architecture between archaeal PolD and multi-subunit RNA polymerases revealed by X-ray crystallography., Nat Commun. 2016 Aug 22;7:12227.
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2016Structural Basis for a New Templated Activity by Terminal Deoxynucleotidyl Transferase: Implications for V(D)J Recombination, Structure 2016 09;24(9):1452-63.
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2016Structural basis of potent Zika-dengue virus antibody cross-neutralization., Nature 2016 Aug; 536(7614): 48-53.
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2016Automated structure modeling of large protein assemblies using crosslinks as distance restraints, Nat. Methods 2016 Jun;13(6):515-20.
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2016A Synthetic Carbohydrate Conjugate Vaccine Candidate against Shigellosis: Improved Bioconjugation and Impact of Alum on Immunogenicity, Bioconjug. Chem. 2016 Mar;.
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2015Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical Analysis, Structure 2016 Jan;24(1):92-104.
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2015Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion., Sci Rep 2015 Sep; 5(): 14223.
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2015Neisseria meningitidis Type IV Pili Composed of Sequence Invariable Pilins Are Masked by Multisite Glycosylation, PLoS Pathog. 2015 Sep;11(9):e1005162.
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2015Improved large-scale prediction of growth inhibition patterns using the NCI60 cancer cell line panel., Bioinformatics 2016 Jan; 32(1): 85-95.
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2015Biogenesis and structure of a type VI secretion membrane core complex, Nature 2015 Jul;523(7562):555-60.
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2015Original 2-(3-Alkoxy-1H-pyrazol-1-yl)azines Inhibitors of Human Dihydroorotate Dehydrogenase (DHODH), J. Med. Chem. 2015 Jul;58(14):5579-98.
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2015Conserved Streptococcus pneumoniae spirosomes suggest a single type of transformation pilus in competence, PLoS Pathog. 2015 Apr;11(4):e1004835.
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2015Structural basis for a novel mechanism of DNA bridging and alignment in eukaryotic DSB DNA repair, EMBO Journal, 2015, 34 (8), pp.1126-1142. ⟨10.15252/embj.201489643⟩.
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2015Programmed chemo-enzymatic synthesis of the oligosaccharide component of a carbohydrate-based antibacterial vaccine candidate, Chem. Commun. (Camb.) 2015 Feb;51(13):2581-4.
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2015A novel bio-orthogonal cross-linker for improved protein/protein interaction analysis, Anal. Chem. 2015 Feb;87(3):1853-60.
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Contact
marie-laure.goupil@pasteur.fr