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Characterisation of molecular interactions
Equilibrium affinity constants (Kd) of protein-protein or protein-ligand interactions can be determined by performing titrations, and monitoring the extrinsic or intrinsic fluorescence of partners by Fluorescence spectroscopy (intensity or anisotropy) or by Microscale thermophoresis […]
Quality control (QC) of purified proteins
This service allows you to obtain a full qualitative analysis (QC) of your purified proteins and helps you to identify conditions in which these proteins are best behaved. The basic analysis will provide you […]
Thermodynamic stability of macromolecules
Circular dichroism (CD) spectroscopy allows the determination of secondary and tertiary structures, the analysis of conformational changes and the measure of thermal and chemical stability of folding. Differential Scanning Calorimetry (DSC) gives access to […]
Secondary and tertiary structures of macromolecules
Circular dichroism (CD) allows the measurement of secondary and tertiary structures, the analyse of conformational changes, the kinetic of folding and the thermal and chemical stability of folding. Fluorescence spectroscopy gives access to the […]
Size and Shape of Macromolecules and their assemblies
PFBMI project submission form Analytical Ultracentrifugation gives access to the mass and shape of macromolecules in solution, allowing to evaluate their oligomerization state, their stoichiometry and the equilibrium constant of assemblies. Dynamic Light Scattering […]
Stoichiometry of Macromolecular Assemblies
Static Light Scattering allows to measure the mass and intrinsic viscosity of macromolecules Analytical Ultracentrifugation gives access to the oligomerization state and the stoichiometry of molecular assemblies
Équipements
Booking site
Bio-Layer Interferometry (BLI) • ForteBio • Octet Red 384
The PFBMI has a high-throughput non-microfluidic BLI instrument (Octet RED384, ForteBio) that enables the label-free analysis of 16 molecular interactions simultaneously in real time. To that avail, one of the interaction partners is immobilized […]
Analytical ultracentrifugation (AUC) • Beckman-Coulter • Optima
The PFBMI has a Optima (Beckman-Coulter) analytical ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, density […]
Mass spectrometer MALDI-TOF/TOF • Bruker • UltrafleXtrem
Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization processes due to its capacity to transfer a moderate excess of energy to the analyte, which is crucial for fragile and non-volatile compounds such as proteins. […]
Differential Scanning Fluorimetry NanoDSF • Nanotemper • Prometheus NT 48
NanoDSF measures the thermal denaturation of proteins by following the modification of the environment of their fluorescent aminoacids Tryptophanes and Tyrosines . Prometheus (Nanotemper) instrument can perform fast and sensitive simultaneous analyses of 48 […]
Infrared spectroscopy (FTIR) • Jasco • 6100
Fourier Transform Infra Red (FTIR) spectroscopy allows to analyze the secondary structure of macromolecules by measuring absorption frequencies of some specific chemical groups (notably, for proteins, that of the amide bond). Informations given by FTIR are […]
Dynamic Light Scattering (DLS) • Wyatt • Dynapro Plate Reader
Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a DynaPro […]
Microscale Thermophoresis (MST) • Nanotemper • NT115
Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]
Microcalorimetry (DSC: Differential Scanning Calorimetry) • Malvern • CAP-DSC
Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. The Capillary Differential scanning calorimeter (CAP-DSC, Malvern) is principally dedicated to the study of the thermal stability of […]
Isothermal Titration Microcalorimetry (ITC) • Malvern • ITC200
Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200) are principally dedicated to the study of molecular interactions. By measuring the […]
Analytical ultracentrifugation (AUC) • Beckman-Coulter • ProteomeLab XL-I
The PFBMI has a ProteomeLab XL-I (Beckman-Coulter) analytical ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, […]
Fluorescence Spectroscopy – Stopped Flow • TgK Scientific • KinetAsyst SF61-DX2
Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]
Fluorescence Spectroscopy • Photon Technology International • Quantamaster QM4CW
Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]
Microscale Thermophoresis (MST) LabelFree • Nanotemper • Monolith LabelFree
Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]
Surface Plasmon Resonance Biacore T200 • GE Healthcare • Biacore T200
The PFBMI has a microfluidic SPR instrument (Biacore T200, GE Healthcare) that enables the label-free analysis of molecular interactions in real time. To that avail, one of the interaction partners is immobilized on a […]
Microcalorimetry – ITC • Malvern • VP-ITC
Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200), principally dedicated to the study of molecular interactions. By measuring the reaction […]
Static Light Scattering and viscometry • Malvern • OMNISEC GPC/SEC system
The intensity of light scattered by a solution is directly proportional to the average molecular weight and to the concentration of its components. The PFBMI has a multiple detector (OMNISEC GPC/SEC, Malvern), coupled to […]
Membres
Patrick England
Responsable de Plateforme
Responsable
Groupes
Platform
Biophysique moléculaire
Patrick England
Anciens Membres
2000
2000
Name
Position
2015
2020
Pascal Lenormand
Technician
2013
2015
Jocelyne Fraysse
Administrative Staff
2016
2021
Christine Detchepare
Administrative Staff
(EN) 2021
(EN) Bruno Baron
(EN) Senior research engineer
Projets
Projet Transversal
Financements
Publications
Télécharger-
2021Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential., Nat Commun 2021 09; 12(1): 5553.
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2021SARS-CoV-2 variant prediction and antiviral drug design are enabled by RBD in vitro evolution., Nat Microbiol 2021 09; 6(9): 1188-1198.
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2021SARS-CoV-2 Nsp3 unique domain SUD interacts with guanine quadruplexes and G4-ligands inhibit this interaction., Nucleic Acids Res 2021 Jul; (): .
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2021SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system., Nat Commun 2021 06; 12(1): 3214.
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2021Quality control of protein reagents for the improvement of research data reproducibility., Nat Commun 2021 05; 12(1): 2795.
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2021A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells., Adv Sci (Weinh) 2021 05; 8(9): 2003630.
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2021Quality control of purified proteins to improve data quality and reproducibility: results from a large-scale survey., Eur Biophys J 2021 May; 50(3-4): 453-460.
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2021RNase R is associated in a functional complex with the RhpA DEAD-box RNA helicase in Helicobacter pylori., Nucleic Acids Res 2021 49:5249-5264.
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2021PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations., Methods Mol Biol 2021 ; 2256(): 89-124.
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2020Deciphering the Unexpected Binding Capacity of the Third PDZ Domain of Whirlin to Various Cochlear Hair Cell Partners., J Mol Biol 2020 11; 432(22): 5920-5937.
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Contact
Phone: 0144389522 (secretary: Christine Detchepare) Email: biophysique@pasteur.fr Address 25, Rue du Docteur Roux 75015, Paris France Booking : PPMS url : https://www.pasteur.fr/ppms/login/?pf=9