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Characterisation of molecular interactions
Equilibrium affinity constants (Kd) of protein-protein or protein-ligand interactions can be determined by performing titrations, and monitoring the extrinsic or intrinsic fluorescence of partners by Fluorescence spectroscopy (intensity or anisotropy) or by Microscale thermophoresis […]
Quality control (QC) of purified proteins
This service allows you to obtain a full qualitative analysis (QC) of your purified proteins and helps you to identify conditions in which these proteins are best behaved. The basic analysis will provide you […]
Thermodynamic stability of macromolecules
Circular dichroism (CD) spectroscopy allows the determination of secondary and tertiary structures, the analysis of conformational changes and the measure of thermal and chemical stability of folding. Differential Scanning Calorimetry (DSC) gives access to […]
Secondary and tertiary structures of macromolecules
Circular dichroism (CD) allows the measurement of secondary and tertiary structures, the analyse of conformational changes, the kinetic of folding and the thermal and chemical stability of folding. Fluorescence spectroscopy gives access to the […]
Size and Shape of Macromolecules and their assemblies
PFBMI project submission form Analytical Ultracentrifugation gives access to the mass and shape of macromolecules in solution, allowing to evaluate their oligomerization state, their stoichiometry and the equilibrium constant of assemblies. Dynamic Light Scattering […]
Stoichiometry of Macromolecular Assemblies
Static Light Scattering allows to measure the mass and intrinsic viscosity of macromolecules Analytical Ultracentrifugation gives access to the oligomerization state and the stoichiometry of molecular assemblies
Équipements
Booking site
Bio-Layer Interferometry (BLI) • ForteBio • Octet Red 384
The PFBMI has a high-throughput non-microfluidic BLI instrument (Octet RED384, ForteBio) that enables the label-free analysis of 16 molecular interactions simultaneously in real time. To that avail, one of the interaction partners is immobilized […]
Analytical ultracentrifugation (AUC) • Beckman-Coulter • Optima
The PFBMI has a Optima (Beckman-Coulter) analytical ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, density […]
Mass spectrometer MALDI-TOF/TOF • Bruker • UltrafleXtrem
Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization processes due to its capacity to transfer a moderate excess of energy to the analyte, which is crucial for fragile and non-volatile compounds such as proteins. […]
Differential Scanning Fluorimetry NanoDSF • Nanotemper • Prometheus NT 48
NanoDSF measures the thermal denaturation of proteins by following the modification of the environment of their fluorescent aminoacids Tryptophanes and Tyrosines . Prometheus (Nanotemper) instrument can perform fast and sensitive simultaneous analyses of 48 […]
Infrared spectroscopy (FTIR) • Jasco • 6100
Fourier Transform Infra Red (FTIR) spectroscopy allows to analyze the secondary structure of macromolecules by measuring absorption frequencies of some specific chemical groups (notably, for proteins, that of the amide bond). Informations given by FTIR are […]
Dynamic Light Scattering (DLS) • Wyatt • Dynapro Plate Reader
Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a DynaPro […]
Microscale Thermophoresis (MST) • Nanotemper • NT115
Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]
Microcalorimetry (DSC: Differential Scanning Calorimetry) • Malvern • CAP-DSC
Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. The Capillary Differential scanning calorimeter (CAP-DSC, Malvern) is principally dedicated to the study of the thermal stability of […]
Isothermal Titration Microcalorimetry (ITC) • Malvern • ITC200
Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200) are principally dedicated to the study of molecular interactions. By measuring the […]
Analytical ultracentrifugation (AUC) • Beckman-Coulter • ProteomeLab XL-I
The PFBMI has a ProteomeLab XL-I (Beckman-Coulter) analytical ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, […]
Fluorescence Spectroscopy – Stopped Flow • TgK Scientific • KinetAsyst SF61-DX2
Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]
Fluorescence Spectroscopy • Photon Technology International • Quantamaster QM4CW
Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]
Microscale Thermophoresis (MST) LabelFree • Nanotemper • Monolith LabelFree
Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]
Surface Plasmon Resonance Biacore T200 • GE Healthcare • Biacore T200
The PFBMI has a microfluidic SPR instrument (Biacore T200, GE Healthcare) that enables the label-free analysis of molecular interactions in real time. To that avail, one of the interaction partners is immobilized on a […]
Microcalorimetry – ITC • Malvern • VP-ITC
Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200), principally dedicated to the study of molecular interactions. By measuring the reaction […]
Static Light Scattering and viscometry • Malvern • OMNISEC GPC/SEC system
The intensity of light scattered by a solution is directly proportional to the average molecular weight and to the concentration of its components. The PFBMI has a multiple detector (OMNISEC GPC/SEC, Malvern), coupled to […]
Membres
Patrick England
Responsable de Plateforme
Responsable
Groupes
Platform
Biophysique moléculaire
Patrick England
Anciens Membres
2000
2000
Name
Position
2015
2020
Pascal Lenormand
Technician
2015
2020
Jocelyne Fraysse
Administrative Staff
Projets
Projet Transversal
Financements
Publications
Télécharger-
2021RNase R is associated in a functional complex with the RhpA DEAD-box RNA helicase in Helicobacter pylori., Nucleic Acids Res 2021 Apr; (): .
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2020Deciphering the Unexpected Binding Capacity of the Third PDZ Domain of Whirlin to Various Cochlear Hair Cell Partners., J Mol Biol 2020 11; 432(22): 5920-5937.
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2020High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics, Nat Biotechnol . 2020 Jun;38(6):715-721. doi: 10.1038/s41587-020-0466-7. Epub 2020 Mar 30..
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2020The anti-IgE mAb omalizumab induces adverse reactions by engaging Fcγ receptors, J. Clin. Invest. 2020 Mar;130(3):1330-1335.
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2020Structure of the prefusion-locking broadly neutralizing antibody RVC20 bound to the rabies virus glycoprotein., Nat Commun 2020 01; 11(1): 596.
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2019X-ray Structures of the Post-fusion 6-Helix Bundle of the Human Syncytins and their Functional Implications., J Mol Biol 2019 12; 431(24): 4922-4940.
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2019Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein., Sci Rep 2019 05; 9(1): 7438.
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2019Regulation of measles virus gene expression by P protein coiled-coil properties, Sci Adv 2019 05;5(5):eaaw3702.
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2019Structural insights into the functional versatility of an FHA domain protein in mycobacterial signaling, Sci Signal 2019 05;12(580).
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2019Histamine releasing factor and elongation factor 1 alpha secreted via malaria parasites extracellular vesicles promote immune evasion by inhibiting specific T cell responses., Cell. Microbiol. 2019 07; 21(7): e13021.
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Contact
Phone: 01 45 68 86 09
Email: biophysique@pasteur.fr
Address
25-28 Rue du Docteur Roux
75015, Paris
France