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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.

Présentation

Pour soumettre une demande de contrôle qualité, veuillez envoyer un e-mail à qcprotein@pasteur.fr. Vous pouvez télécharger et joindre un formulaire de soumission QC.

Pour tous les autres services PFBMI, veuillez envoyer un e-mail à biophysique@pasteur.fr accompagné d’un formulaire de soumission de projet complété.

To consult the English version, please follow this link: https://research.pasteur.fr/en/team/biophysics/

La PFBMI est labellisée ISO 9001.

Pour plus d’information sur la Direction de la Technologie, voir ici.

 

Services

Booking site

Characterisation of molecular interactions

Equilibrium affinity constants (Kd) of protein-protein or protein-ligand interactions can be determined by performing  titrations, and monitoring  the extrinsic or intrinsic fluorescence of partners by Fluorescence spectroscopy (intensity or anisotropy) or by Microscale thermophoresis […]

Quality control (QC) of purified proteins

This service allows you to obtain a full qualitative analysis (QC) of your purified proteins and helps you to identify conditions in which these proteins are best behaved. The basic analysis will provide you […]

Thermodynamic stability of macromolecules

Circular dichroism (CD) spectroscopy allows the determination of secondary and tertiary structures, the analysis of conformational changes and the measure of thermal and chemical stability of folding. Differential Scanning Calorimetry (DSC) gives access to […]

Secondary and tertiary structures of macromolecules

Circular dichroism (CD) allows the measurement of secondary and tertiary structures, the analyse of conformational changes, the kinetic of folding and the thermal and chemical stability of folding. Fluorescence spectroscopy gives access to the […]

Size and Shape of Macromolecules and their assemblies

Analytical Ultracentrifugation gives access to the mass and shape of macromolecules in solution, allowing to evaluate their oligomerization state, their stoichiometry and the equilibrium constant of assemblies. Dynamic Light Scattering (cuvette or microplaque) allows […]

Stoichiometry of Macromolecular Assemblies

Static Light Scattering allows to measure the mass and intrinsic viscosity of macromolecules Analytical Ultracentrifugation gives access to the oligomerization state and the stoichiometry of molecular assemblies  

Équipements

Booking site

Fluorescence spectroscopy • Jasco • FP-8550

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Taylor Dispersion & Viscometry • Malvern Panalytical • Viscosizer TD

A Taylor dispersion instrument allows the determination of the diffusion coefficient, the hydrodynamic radius and the intrinsic viscosity of molecules. The method measures the dispersion of a solute pulse injected into a flowing fluid […]

Mass Photometry • Refeyn • TwoMP

Mass Photometry is offered using the Refeyn TwoMP system. This applies the principle of interference reflection microscopy and interferometric scattering microscopy to quantify light scattered by a single molecule on a glass surface. The […]

Bio-Layer Interferometry (BLI) • ForteBio • Octet Red 384

The PFBMI has a high-throughput non-microfluidic BLI instrument (Octet RED384, ForteBio) that enables the label-free analysis of 16 molecular interactions simultaneously in real time. To that avail, one of the interaction partners is immobilized […]

Analytical ultracentrifugation (AUC) • Beckman-Coulter • Optima

The PFBMI has a Optima (Beckman-Coulter) analytical  ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, density […]

Mass spectrometer MALDI-TOF/TOF • Bruker • UltrafleXtreme

Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization processes due to its capacity to transfer a moderate excess of energy to the analyte, which is crucial for fragile and non-volatile compounds such as proteins. […]

Differential Scanning Fluorimetry NanoDSF • Nanotemper • Prometheus NT 48

NanoDSF measures the thermal denaturation of proteins by following the modification of the  environment of their fluorescent aminoacids Tryptophanes and Tyrosines . Prometheus (Nanotemper) instrument can perform fast and sensitive simultaneous analyses of 48 […]

Infrared spectroscopy (FTIR) • Jasco • 6100

Fourier Transform Infra Red (FTIR) spectroscopy allows to analyze the secondary structure of macromolecules by measuring absorption frequencies of some specific chemical groups (notably, for proteins, that of the amide bond). Informations given by FTIR are […]

Dynamic Light Scattering (DLS) • Wyatt • Dynapro Plate Reader

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a DynaPro […]

Microscale Thermophoresis (MST) • Nanotemper • NT115

Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]

Microcalorimetry (DSC: Differential Scanning Calorimetry) • Malvern • CAP-DSC

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. The Capillary Differential scanning calorimeter (CAP-DSC, Malvern) is principally dedicated to the study of the thermal stability of […]

Isothermal Titration Microcalorimetry (ITC) • Malvern • ITC200

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200) are principally dedicated to the study of molecular interactions. By measuring the […]

Analytical ultracentrifugation (AUC) • Beckman-Coulter • ProteomeLab XL-I

The PFBMI has a ProteomeLab XL-I (Beckman-Coulter) analytical  ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, […]

Fluorescence Spectroscopy – Stopped Flow • TgK Scientific • KinetAsyst SF61-DX2

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Fluorescence Spectroscopy • Photon Technology International • Quantamaster QM4CW

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Microscale Thermophoresis (MST) LabelFree • Nanotemper • Monolith LabelFree

Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]

Surface Plasmon Resonance Biacore T200 • GE Healthcare • Biacore T200

The PFBMI has a microfluidic SPR instrument (Biacore T200, Cytiva) that enables the label-free analysis of molecular interactions in real time. To that avail, one of the interaction partners is immobilized on a sensorchip, […]

Microcalorimetry – ITC • Malvern • VP-ITC

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200), principally dedicated to the study of molecular interactions. By measuring the reaction […]

Static Light Scattering and viscometry • Malvern • OMNISEC GPC/SEC system

The intensity of light scattered by a solution is directly proportional to the average molecular weight and to the concentration of its components. The PFBMI has a multiple detector (OMNISEC GPC/SEC, Malvern), coupled to […]

Dynamic Light Scattering • Wyatt • Dynapro MS800

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a cuvette […]

Circular Dichroism • JASCO • J-1500/ 450W

The PFBMI has an J-1500/ 450W instrument. Studying macromolecules (and in particular proteins) by circular dichroism allows to obtain information about their folding. For proteins, far UV (180-260 nm) and near UV (250-330 nm) […]

Anciens Membres

2000
2000
Name
Position
2015
2020
Pascal Lenormand
Technician
2013
2015
Jocelyne Fraysse
Administrative Staff
2016
2021
Christine Detchepare
Administrative Staff
2014
2021
Bruno Baron
Senior research engineer

Projets

Projet
En cours
8 Membres

Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA

Alexandre Chenal (PI)

This project aims to describe the hydrodynamic and thermodynamic properties of the CyaA toxin and the conformational changes it undergoes during its translocation process across the lipid membranes of Bordetella pertussis (secretion) and of […]
Projet
En cours
3 Membres

CarbUniVax

Laurence Mulard (PI)

At the forefront of the rising fields of Chemical and Synthetic Biology, CarbUniVax is a multidisciplinary project sitting at the frontier between Glycosciences and Vaccinology. A multidisciplinary strategy is proposed that challenges ambitious goals […]
Projet
En cours
10 Membres

Inosine-5′-monophosphate dehydrogenase

Hélène Munier-Lehmann (PI)

 Inosine-5′-monophosphate dehydrogenase (IMPDH) is ubiquitously essential for the biosynthesis of guanosine nucleotides and catalyzes the NAD-dependent oxidation of IMP to XMP. IMPDH is a major target in the design of both antitumor and immunosuppresive […]
Projet
En cours
26 Membres

CyaA secretion, folding and translocation across membrane

Alexandre Chenal (PI)

CyaA, a 1706 residue-long protein, is one of the major virulence factors produced by B. pertussis and plays an important role in the early stages of respiratory tract colonization. This toxin uses an original […]

Financements

Principales publications

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