Tapez votre recherche ici
  • Équipes
  • Membres
  • Projets
  • Événements
  • Appels
  • Emplois
  • publications
  • Logiciel
  • Outils
  • Réseau
  • Équipement

Un petit guide pour l'utilisation de la recherche avancée :

  • Tip 1. Utilisez "" afin de chercher une expression exacte.
    Exemple : "division cellulaire"
  • Tip 2. Utilisez + afin de rendre obligatoire la présence d'un mot.
    Exemple : +cellule +stem
  • Tip 3. Utilisez + et - afin de forcer une inclusion ou exclusion d'un mot.
    Exemple : +cellule -stem
e.g. searching for members in projects tagged cancer
Rechercher
Compteur
IN
OUT
Contenu 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Personnel Administratif
  • Chargé(e) de Recherche Expert
  • Directeur(trice) de Recherche
  • Assistant(e) de Recherche Clinique
  • Professeur(e)
  • Etudiant(e) M2
  • Aide technique
  • Chercheur(euse) Contractuel(le)
  • Chercheur(euse) Permanent(e)
  • Pharmacien(ne)
  • Etudiant(e) en thèse
  • Médecin
  • Post-doctorant(e)
  • Chef(fe) de Projet
  • Chargé(e) de Recherche
  • Ingénieur(e) de Recherche
  • Chercheur(euse) Retraité(e)
  • Technicien(ne)
  • Etudiant(e)
  • Vétérinaire
  • Visiteur(euse) Scientifique
  • Directeur(trice) Adjoint(e) de Centre
  • Directeur(trice) Adjoint(e) de Départment
  • Directeur(trice) Adjoint(e) de Centre National de Référence
  • Directeur(trice) Adjoint(e) de Plateforme
  • Directeur(trice) de Centre
  • Directeur(trice) de Départment
  • Directeur(trice) d'Institut
  • Directeur(trice) de Centre National de Référence
  • Chef(fe) de Groupe
  • Responsable de Plateforme
  • Responsable opérationnel et administratif
  • Responsable de Structure
  • Président(e) d'honneur de Département
  • Coordinateur(trice) du Labex
Contenu 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Personnel Administratif
  • Chargé(e) de Recherche Expert
  • Directeur(trice) de Recherche
  • Assistant(e) de Recherche Clinique
  • Professeur(e)
  • Etudiant(e) M2
  • Aide technique
  • Chercheur(euse) Contractuel(le)
  • Chercheur(euse) Permanent(e)
  • Pharmacien(ne)
  • Etudiant(e) en thèse
  • Médecin
  • Post-doctorant(e)
  • Chef(fe) de Projet
  • Chargé(e) de Recherche
  • Ingénieur(e) de Recherche
  • Chercheur(euse) Retraité(e)
  • Technicien(ne)
  • Etudiant(e)
  • Vétérinaire
  • Visiteur(euse) Scientifique
  • Directeur(trice) Adjoint(e) de Centre
  • Directeur(trice) Adjoint(e) de Départment
  • Directeur(trice) Adjoint(e) de Centre National de Référence
  • Directeur(trice) Adjoint(e) de Plateforme
  • Directeur(trice) de Centre
  • Directeur(trice) de Départment
  • Directeur(trice) d'Institut
  • Directeur(trice) de Centre National de Référence
  • Chef(fe) de Groupe
  • Responsable de Plateforme
  • Responsable opérationnel et administratif
  • Responsable de Structure
  • Président(e) d'honneur de Département
  • Coordinateur(trice) du Labex
Recherche
Revenir
Haut de page
Partagez

Présentation

La Plate Forme de Biophysique MoleculaIre, PFBMI  réunit un ensemble d’une vingtaine d’équipements de pointe destinés à la caractérisation physico-chimique in vitro des propriétés intrinsèques des macromolécules biologiques en solution et de leurs assemblages.

Le personnel de la PFBMI possède un véritable savoir-faire technique et méthodologique permettant d’aider tout scientifique à aborder des questions telles que la stœchiométrie, la stabilité ou le repliement de protéines ainsi que les propriétés de leurs interactions (paramètres thermodynamiques et cinétiques, etc…

Plus d’informations sont disponibles sur notre page web en langue anglaise.

 

Services

Booking site

Characterisation of molecular interactions

Equilibrium affinity constants (Kd) of protein-protein or protein-ligand interactions can be determined by performing  titrations, and monitoring  the extrinsic or intrinsic fluorescence of partners by Fluorescence spectroscopy (intensity or anisotropy) or by Microscale thermophoresis […]

Quality control (QC) of purified proteins

This service allows you to obtain a full qualitative analysis (QC) of your purified proteins and helps you to identify conditions in which these proteins are best behaved. The basic analysis will provide you […]

Thermodynamic stability of macromolecules

Circular dichroism (CD) spectroscopy allows the determination of secondary and tertiary structures, the analysis of conformational changes and the measure of thermal and chemical stability of folding. Differential Scanning Calorimetry (DSC) gives access to […]

Secondary and tertiary structures of macromolecules

Circular dichroism (CD) allows the measurement of secondary and tertiary structures, the analyse of conformational changes, the kinetic of folding and the thermal and chemical stability of folding. Fluorescence spectroscopy gives access to the […]

Size and Shape of Macromolecules and their assemblies

PFBMI project submission form Analytical Ultracentrifugation gives access to the mass and shape of macromolecules in solution, allowing to evaluate their oligomerization state, their stoichiometry and the equilibrium constant of assemblies. Dynamic Light Scattering […]

Stoichiometry of Macromolecular Assemblies

Static Light Scattering allows to measure the mass and intrinsic viscosity of macromolecules Analytical Ultracentrifugation gives access to the oligomerization state and the stoichiometry of molecular assemblies  

Équipements

Booking site

Bio-Layer Interferometry (BLI) • ForteBio • Octet Red 384

The PFBMI has a high-throughput non-microfluidic BLI instrument (Octet RED384, ForteBio) that enables the label-free analysis of 16 molecular interactions simultaneously in real time. To that avail, one of the interaction partners is immobilized […]

Analytical ultracentrifugation (AUC) • Beckman-Coulter • Optima

The PFBMI has a Optima (Beckman-Coulter) analytical  ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, density […]

Mass spectrometer MALDI-TOF/TOF • Bruker • UltrafleXtrem

Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization processes due to its capacity to transfer a moderate excess of energy to the analyte, which is crucial for fragile and non-volatile compounds such as proteins. […]

Differential Scanning Fluorimetry NanoDSF • Nanotemper • Prometheus NT 48

NanoDSF measures the thermal denaturation of proteins by following the modification of the  environment of their fluorescent aminoacids Tryptophanes and Tyrosines . Prometheus (Nanotemper) instrument can perform fast and sensitive simultaneous analyses of 48 […]

Infrared spectroscopy (FTIR) • Jasco • 6100

Fourier Transform Infra Red (FTIR) spectroscopy allows to analyze the secondary structure of macromolecules by measuring absorption frequencies of some specific chemical groups (notably, for proteins, that of the amide bond). Informations given by FTIR are […]

Dynamic Light Scattering (DLS) • Wyatt • Dynapro Plate Reader

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a DynaPro […]

Microscale Thermophoresis (MST) • Nanotemper • NT115

Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]

Microcalorimetry (DSC: Differential Scanning Calorimetry) • Malvern • CAP-DSC

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. The Capillary Differential scanning calorimeter (CAP-DSC, Malvern) is principally dedicated to the study of the thermal stability of […]

Isothermal Titration Microcalorimetry (ITC) • Malvern • ITC200

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200) are principally dedicated to the study of molecular interactions. By measuring the […]

Analytical ultracentrifugation (AUC) • Beckman-Coulter • ProteomeLab XL-I

The PFBMI has a ProteomeLab XL-I (Beckman-Coulter) analytical  ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, […]

Fluorescence Spectroscopy – Stopped Flow • TgK Scientific • KinetAsyst SF61-DX2

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Fluorescence Spectroscopy • Photon Technology International • Quantamaster QM4CW

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Microscale Thermophoresis (MST) LabelFree • Nanotemper • Monolith LabelFree

Microscale thermophoresis allows to monitor a great variety of molecular interactions rapidly and with a very low sample consumption, by measuring optically the mobility of macromolecules in a microscopic temperature gradient generated in a […]

Surface Plasmon Resonance Biacore T200 • GE Healthcare • Biacore T200

The PFBMI has a microfluidic SPR instrument (Biacore T200, GE Healthcare) that enables the label-free analysis of molecular interactions in real time. To that avail, one of the interaction partners is immobilized on a […]

Microcalorimetry – ITC • Malvern • VP-ITC

Microcalorimetry enables the thermodynamic characterization of molecular interactions in solution, and of biochemical reactions in general. Isothermal titration calorimeters (VP-ITC et ITC200), principally dedicated to the study of molecular interactions. By measuring the reaction […]

Static Light Scattering and viscometry • Malvern • OMNISEC GPC/SEC system

The intensity of light scattered by a solution is directly proportional to the average molecular weight and to the concentration of its components. The PFBMI has a multiple detector (OMNISEC GPC/SEC, Malvern), coupled to […]

Dynamic Light Scattering • Wyatt • Dynapro MS800

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a cuvette […]

Circular Dichroism • AVIV • 215

The PFBMI has an Aviv 215 instrument with accessories for the measurement of total fluorescence and to perform titrations. Studying macromolecules (and in particular proteins) by circular dichroism allows to obtain information about their […]

Anciens Membres

2000
2000
Name
Position
2015
2020
Pascal Lenormand
Technician
2015
2020
Jocelyne Fraysse
Administrative Staff

Projets

Financements

Publications

Télécharger

Contact

Phone: 01 45 68 86 09
Email: biophysique@pasteur.fr

Address
25-28 Rue du Docteur Roux
75015, Paris
France