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Physician
Post-doc
Project Manager
Research Associate
Research Engineer
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Technician
Undergraduate Student
Veterinary
Visiting Scientist

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Deputy Director of Department
Deputy Director of National Reference Center
Deputy Head of Facility
Director of Center
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Director of Institute
Director of National Reference Center
Group Leader
Head of Facility
Head of Operations
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Honorary President of the Departement
Labex Coordinator

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© Institut Pasteur

Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.

Member: Research Engineer

Bertrand Raynal

• Molecular Biophysics

Platform: Molecular Biophysics

Scientific Fields

Diseases

Organisms

Applications

Technique

Technique

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55 Publications

Projects

SUPERCPLX

Marco Bellinzoni (PI)

Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]

Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA

Alexandre Chenal (PI)

This project aims to describe the hydrodynamic and thermodynamic properties of the CyaA toxin and the conformational changes it undergoes during its translocation process across the lipid membranes of Bordetella pertussis (secretion) and of […]

Molecular crowding

Alexandre Chenal (PI)

We are interested in investigating the effects of molecular crowding, i.e., excluded volume effects, mimicking the effects of high concentrations of macromolecular solutes found in biological fluids on biochemical properties of proteins. In particular, […]

In solution conformation of the SigmaS subunit of RNA polymerase

Françoise Norel-Bozouklian (PI)

There is no tridimentional structure available for free sigma factors. We have recently probed the conformation of SigmaS in solution by analytical ultracentrifugation, small angle X-ray scattering and Hydrogen-Deuterium exchange coupled with mass spectrometry.

CyaA secretion, folding and translocation across membrane

Alexandre Chenal (PI)

CyaA, a 1706 residue-long protein, is one of the major virulence factors produced by B. pertussis and plays an important role in the early stages of respiratory tract colonization. This toxin uses an original […]

CV

BIOPHYSIC EXPERT ENGINEER

Expert in molecular hydrodynamic characterization

Coordinator of the protein quality control service of Institut Pasteur

Career

2007 onwards Engineer in charge of the “hydrodynamic characterization of macromolecules” section, Centre of Biophysics of Macromolecules and their Interactions (PFBMI), Institut Pasteur, Paris.

2004-2007 Postdoctoral Research associate University of (supervisors: Prof. Cay Kielty and Dr Adrian Shuttleworth) Manchester, UK Fibrillin structure and its organisation in microfibrils.

2003-2004 Postdoctoral Research associate (supervisor: Dr Dave Thornton) University of Organisation of normal and pathological mucus (cystic fibrosis, asthma…) and Manchester, UK influence of calcium.

1999-2003 Research Assistant (supervisors: Prof. John Sheehan and Prof. Tim Hardingham) University of The molecular structure, organisation and interactions in mucus gels. Manchester, UK

Teaching in the last 5 years

Since 2011 Hydrodynamic (16 hours / year) Institut Pasteur Protein Biochemistry course

Scientific workshops in the last 5 years

January 2020 FEBS INSTRUCT MOBIEU Practical course: Hydrodynamic and thermodynamic analysis of biological macromolecules and their interactions

October 2019 ARBRE-MOBIEU/INSTRUCT quality control of samples for structural biology training school

Sine 2016 Integrative structural biology course

Education

2003 PhD in biochemistry University of Manchester

1999 MSc in biochemistry, Université Paul Sabatier Toulouse III ERASMUS training period at the University of Manchester

Publications

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Published on: 06 Feb 2015 • Modified on: 24 Mar 2020