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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.

Projects

project
Ongoing
7 Members

SUPERCPLX

Marco Bellinzoni (PI)

Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]
project
Ongoing
8 Members

Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA

Alexandre Chenal (PI)

This project aims to describe the hydrodynamic and thermodynamic properties of the CyaA toxin and the conformational changes it undergoes during its translocation process across the lipid membranes of Bordetella pertussis (secretion) and of […]
project
Ongoing
15 Members

Molecular crowding

Alexandre Chenal (PI)

We are interested in investigating the effects of molecular crowding, i.e., excluded volume effects, mimicking the effects of high concentrations of macromolecular solutes found in biological fluids on biochemical properties of proteins. In particular, […]
project
Completed
5 Members

In solution conformation of the SigmaS subunit of RNA polymerase

Françoise Norel-Bozouklian (PI)

There is no tridimentional structure available for free sigma factors.   We have recently probed the conformation of SigmaS in solution by analytical ultracentrifugation, small angle X-ray scattering and Hydrogen-Deuterium exchange coupled with mass spectrometry.
project
Ongoing
26 Members

CyaA secretion, folding and translocation across membrane

Alexandre Chenal (PI)

CyaA, a 1706 residue-long protein, is one of the major virulence factors produced by B. pertussis and plays an important role in the early stages of respiratory tract colonization. This toxin uses an original […]

CV

Education

1999     MSc in biochemistry, Université Paul Sabatier Toulouse III ERASMUS training period at the University of Manchester

2003     PhD in biochemistry University of Manchester

Career

2007 onwards   Engineer in charge of the “hydrodynamic characterization of macromolecules” section, Centre of Biophysics of Macromolecules and their Interactions (PFBMI), Institut Pasteur, Paris.

2004-2007         Postdoctoral Research associate University of (supervisors: Prof. Cay Kielty and Dr Adrian Shuttleworth) Manchester, UK Fibrillin structure and its organisation in microfibrils.

2003-2004         Postdoctoral Research associate (supervisor: Dr Dave Thornton) University of Organisation of normal and pathological mucus (cystic fibrosis, asthma…) and Manchester, UK influence of calcium.

1999-2003         Research Assistant (supervisors: Prof. John Sheehan and Prof. Tim Hardingham) University of The molecular structure, organisation and interactions in mucus gels. Manchester, UK

Teaching in the last 5 years

July 2012         Centrifugation: from a purification method to an analytical method (1 day)Beckman Coulter (Villepinte)

Since 2011       Analytical ultracentrifugation (12 hours / year) Institut Pasteur Protein Biochemistry course

Organization/ chairing of scientific workshops, conferences and courses in the last 5 years

July 2011         Hydroprot 11 2nd international symposium “Macromolecular hydrodynamics, from aqueous solutions to living cells”, Institut Pasteur, Paris, France

Publications