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  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Service

Size and Shape of Macromolecules and their assemblies

Starting Date
30
Mar 2015
Status
Ongoing
Members
2
Structures
1
Internal price: 2
Academic price: 1

About

Analytical Ultracentrifugation gives access to the mass and shape of macromolecules in solution, allowing to evaluate their oligomerization state, their stoichiometry and the equilibrium constant of assemblies.

Dynamic Light Scattering (cuvette or microplaque) allows to characterise the homogeneity of macromolecular samples and to determine the hydrodynamic radius of the species in solution.

Static Light Scattering and viscosimetry give access to the mass and intrinsic viscosity of macromolecules, allowing to estimate their oligomerisation state and the stoichiometry of assemblies.

 

Access Protocol

PFBMI equipments are available for all scientists worldwide, but a priority is given to members of Institut Pasteur and the Institut Pasteur International Network.
Requests can be submitted at any time  (biophysique@pasteur.fr) attaching a project submission form.

Equipments

Analytical ultracentrifugation (AUC) • Beckman-Coulter • ProteomeLab XL-I

The PFBMI has a ProteomeLab XL-I (Beckman-Coulter) analytical  ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, […]

Dynamic Light Scattering • Wyatt • Dynapro MS800

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a cuvette […]

Static Light Scattering and viscometry • Malvern • OMNISEC GPC/SEC system

The intensity of light scattered by a solution is directly proportional to the average molecular weight and to the concentration of its components. The PFBMI has a multiple detector (OMNISEC GPC/SEC, Malvern), coupled to […]