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  • Director of Center
  • Director of Department
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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Service

Size and Shape of Macromolecules and their assemblies

Starting Date
30
Mar 2015
Status
Ongoing
Members
1
Structures
1
Internal price: 2
Academic price: 1

About

PFBMI project submission form

Dynamic Light Scattering (cuvette or microplaque) allows to quantify the diffusion speed of macromolecules in solution and than to deduce their hydrodynamic radius.diffusion-dynamique-de-la-lumiere-dls300plate reader  

Static Light Scattering and viscosimetry give access to mass and intrinsic viscosity of macromolecules, allowing to estimate the oligomerisation state and the stoechiometry of assemblies.

photo-viscotek-gpcmax-tda550

 

Analytical Ultracentrifugation give access to the mass and shape of macromolecules in solution, allowing to evaluate their oligomerization state, their stoechiometry and the equilibrium constant of assemblies. ultra300  

Access Protocol

Equipments on the PFBMI are available for the entire scientific community, national and international, but a priority is given at Institut Pasteur and Institut Pasteur International Network. The PFBMI is performing the analysis as one goes along their reception (biophysique@pasteur.fr). An inital form (upload here) must be joined.

Equipments

Analytical ultracentrifugation • Beckman-Coulter • ProteomeLab XL-I

The PFBMI has a ProteomeLab XL-I (Beckman-Coulter) analytical  ultracentrifuge with a dual UV/visible and interference detection system. The hydrodynamic behavior of a macromolecule and its sedimentation coefficient are determined by different parameters: volume, shape, […]

Dynamic Light Scattering • Wyatt • Dynapro MS800

Studying the dynamic light scattering of particles in solution allows to quantify their diffusion coefficient and to deduce their hydrodynamic radius (which depends notably on their mass and shape). The PFBMI has a cuvette […]

Static Light Scattering and viscometry • Viscotek • TDA 302

The intensity of light scattered by a solution is directly proportional to the average molecular weight and to the concentration of its components. The PFBMI has a multiple detector (Viscotek TDAmax, Malvern), coupled to […]