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  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinician Researcher
  • Department Manager
  • Full Professor
  • Graduate Student
  • Honorary Professor
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Equipment

Mass Photometry

Scientific Fields
Diseases
Organisms
Applications
Technique

Refeyn • TwoMP

Mass Photometry is offered using the Refeyn TwoMP system. This applies the principle of interference reflection microscopy and interferometric scattering microscopy to quantify light scattered by a single molecule on a glass surface. The amount of light scattered by a particle scales linearly with the particle’s volume and refractive index. The amount of light scattered by each molecule is directly correlated to its molecular mass.

Based on the above principle, the Refeyn TwoMP can monitor protein-complex stoichiometry at a single-molecule level with high-sensitivity (1-10nM) and can at the same time determine molecular weight of proteins, membrane proteins, DNA, RNA, nanodiscs etc. in a 30kDa-5MDa range and with good accuracy.