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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Equipment

Bio-Layer Interferometry (BLI)

Scientific Fields
Diseases
Organisms
Applications
Technique

ForteBio • Octet Red 384

The PFBMI has a high-throughput non-microfluidic BLI instrument (Octet RED384, ForteBio) that enables the label-free analysis of 16 molecular interactions simultaneously in real time. To that avail, one of the interaction partners is immobilized (covalently or non-covalently) on a sensor, which is then dipped into a microplate containing the other interaction partner. This technique allows to determine the rates of association (kon) and dissociation (koff) between biological macromolecules. Our instrument is entirely automatable, which allows to perform large series of highly reproducible experiments.