The Mouse Genetics Engineering Center (Centre d’Ingénierie Génétique Murine, CIGM) is the Institut Pasteur’s transgenic mouse core facility. Since its creation in 2003, the CIGM aims to generate new models of transgenic mice by classical or targeted transgenesis. Classical (additive) transgenesis, which includes lentiviral transgenesis, is based on a random insertion of an exogenous gene. Targeted transgenesis consists in a deletion or a specific modification in an endogenous locus using homologous recombination in Embryonic Stem (ES) cells or, more recently, by directly using specific nucleases. Preferentially used by the Institut Pasteur scientific community, the services of CIGM are also available to outside customers interested in new transgenic, knock-down, knock-out (KO) or knock-in (KI) mouse/rat models. The core facility also provides expert advice regarding the design of gene constructs suitable for additive/targeted transgenesis and homologous recombination experiments.
Equipments
Embryo Microinjection Station • Nikon TE2000 •
Microscope +Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system (manual at the microscope level) allow, like the ASTP station above-mentioned, the storage and recall of positions and the […]
Embryo Reimplantation Station • Nikon Stereomicroscopes SMZ800 (episcopic base/diascopic base) •
The diascopic stereomicroscope allows embryo counting and the preparation of the reimplantation pipette. The stereomicroscope coupled to a coaxial Episcopic Illuminator (Fibber Optic Light Source) offers bright illumination over the entire surface of the […]
Eukaryotic Cell Electroporator • Bio-Rad Gene Pulser X Cell Total System •
The electroporator (with the appropriated parameters) gives the necessary pulse for the entering of the gene targeting vector or recombinase-expressing plasmid into the Embryonic Stem (ES) Cells.
XY Clone Laser System • Hamilton Thorne Bioscience •
The laser module, attached just like a typical objective, permits non-contact ablation of targeted membranes. It is mainly used in transgenesis for ablation of the zona pellucida for microinjection of eight-cell and morula stage […]
Piezo Impact Micromanipulator • PRIME TECH, Dipsi Industrie •
The piezo-assisted microinjection increases success rates for transgenesis by IntraCytoplasmic Sperm Injection (ICSI), where sperm are coated with exogenous DNA and injected into oocytes. The piezo impact drive, attached to the injecting micropipette holder, […]
Embryo Microinjection Station • Leica ASTP •
Station including Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system combines the operation of both microscope and micromanipulators into one user-oriented control unit (first motorized integrated transgenic platform). […]
Members
Former Members
2000
2000
Name
Position
2013
2015
Carine Moigneu
Technician
2007
2017
Gaëlle Chauveau-Le Friec
Technician
2017
2019
Laetitia Joullie
Technician
Projects
Publications
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2022An intranasal lentiviral booster reinforces the waning mRNA vaccine-induced SARS-CoV-2 immunity that it targets to lung mucosa., Mol Ther 2022 Apr; (): .
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2021Brain cross-protection against SARS-CoV-2 variants by a lentiviral vaccine in new transgenic mice., EMBO Mol Med 2021 12; 13(12): e14459.
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2021De novo germline mutation in the dual specificity phosphatase 10 gene accelerates autoimmune diabetes., Proc Natl Acad Sci U S A 2021 11; 118(47): .
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2021The antitumoral effects of chemerin are independent from leukocyte recruitment and mediated by inhibition of neoangiogenesis., Oncotarget 2021 Sep; 12(19): 1903-1919.
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2021The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium., Nat Commun 2021 08; 12(1): 4810.
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2021Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse., Skelet Muscle 2021 Feb; 11(1): 5.
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2021The ring finger protein 213 gene (Rnf213) contributes to Rift Valley fever resistance in mice., Mamm Genome 2021 02; 32(1): 30-37.
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2021Dissection of the Fgf8 regulatory landscape by in vivo CRISPR-editing reveals extensive intra- and inter-enhancer redundancy., Nat Commun 2021 01; 12(1): 439.
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2021Lysosomal Acid Lipase Drives Adipocyte Cholesterol Homeostasis and Modulates Lipid Storage in Obesity, Independent of Autophagy., Diabetes 2021 01; 70(1): 76-90.
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2020Mitochondrial AIF loss causes metabolic reprogramming, caspase-independent cell death blockade, embryonic lethality, and perinatal hydrocephalus., Mol Metab 2020 10; 40(): 101027.
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Contact
Phone: 01 44 38 95 66 Email: francina.langa-vives@pasteur.fr Centre d’Ingénierie Génétique Murine Bâtiment François Jacob 1er étage 28, rue du Dr. Roux 75015, Paris France