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  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Director of Center
  • Director of Department
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Scientific Fields
Diseases
Organisms
Applications
Technique

About

The Mouse Genetics Engineering Center (Centre d’Ingénierie Génétique Murine, CIGM) is the Institut Pasteur’s transgenic mouse core facility. Since its creation in 2003, the CIGM aims to generate new models of transgenic mice by classical or targeted transgenesis. Classical (additive) transgenesis, which includes lentiviral transgenesis, is based on a random insertion of an exogenous gene. Targeted transgenesis consists in a deletion or a specific modification in an endogenous locus using homologous recombination in Embryonic Stem (ES) cells or, more recently, by directly using specific nucleases. Preferentially used by the Institut Pasteur scientific community, the services of CIGM are also available to outside customers interested in new transgenic, knock-down, knock-out (KO) or knock-in (KI) mouse/rat models. The core facility also provides expert advice regarding the design of gene constructs suitable for additive/targeted transgenesis and homologous recombination experiments.

Projects

project
ongoing
4 Members

Rat transgenesis

Francina Langa Vives (PI)

We have an increasing experience in modification on rat genome using lentiviral vectors and Zinc Finger Nucleases (ZFNs). Rat models are more convenient for surgery, due to their medium size, and constitute a robust […]
project
ongoing
4 Members

Genome modifications by CRISPR/cas9 system

Francina Langa Vives (PI)

The microinjection of the CRISPR/Cas9 system in one-cell embryos is actually revolutionizing the in vivo genome-engineering field because of its big efficiency for KO/ KI (when a donor DNA matrix co-injected with the CRISPR/CAS9 […]
project
ongoing
4 Members

Gene editing by specific nucleases (zinc finger nucleases-zfns, tale nucleases, CRISPR/Cas9 system)

Francina Langa Vives (PI)

The nucleases (ZFN pairs, TALE pairs, Cas9/CRISPR system) are microinjected, mainly in its mRNA form, into the pronucleus and/or the cytoplasm of one-cell mouse/rat embryos in order to get the desired targeted modification. These […]
project
ongoing
4 Members

Microinjection of “External” Mutant ES Cells And Subsequently Germ-Line Chimera Generation

Francina Langa Vives (PI)

The easy availability of modified ES cells obtained from KOMP and EUCOMM high-throughput gene-targeting mutagenesis programs (mainly JM8.N4, JM8 A3.N1 ES cell lines) has considerably increased the requests for these direct microinjections in the […]
project
ongoing
4 Members

Lentiviral Transgenesis

Francina Langa Vives (PI)

Lentiviral transgenesis is a special type of classical/additive transgenesis. The transgene, inserted in a lentiviral construct, is microinjected directly under the zona pellucida of one-cell embryos. Then viruses are able to enter directly the […]
project
ongoing
4 Members

Targeted Transgenesis By Homologous Recombination In Embryonic Stem (ES) Cells

Francina Langa Vives (PI)

A gene targeting vector is transfected into the desired mouse ES cells to modify an endogenous gene by homologous recombination. The mutant ES cells thus obtained are subsequently microinjected into mouse blastocysts for the […]
project
ongoing
4 Members

Classical/Additive Transgenesis by Microinjection

Francina Langa Vives (PI)

This type of transgenesis consists in the microinjection of the gene of interest into the pronucleus of fertilized eggs to generate transgenic mice expressing the transgene. The microinjected transgenes, which integrated randomly into the […]

Equipments

Embryo Microinjection Station • Nikon TE2000 •

Microscope +Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system (manual at the microscope level) allow, like the ASTP station above-mentioned, the storage and recall of positions and the […]

Embryo Reimplantation Station • Nikon Stereomicroscopes SMZ800 (episcopic base/diascopic base) •

The diascopic stereomicroscope allows embryo counting and the preparation of the reimplantation pipette. The stereomicroscope coupled to a coaxial Episcopic Illuminator (Fibber Optic Light Source) offers bright illumination over the entire surface of the […]

Eukaryotic Cell Electroporator • Bio-Rad Gene Pulser X Cell Total System •

The electroporator (with the appropriated parameters) gives the necessary pulse for the entering of the gene targeting vector or recombinase-expressing plasmid into the Embryonic Stem (ES) Cells.

XY Clone Laser System • Hamilton Thorne Bioscience •

The laser module, attached just like a typical objective, permits non-contact ablation of targeted membranes. It is mainly used in transgenesis for ablation of the zona pellucida for microinjection of eight-cell and morula stage […]

Piezo Impact Micromanipulator • PRIME TECH, Dipsi Industrie •

The piezo-assisted microinjection increases success rates for transgenesis by IntraCytoplasmic Sperm Injection (ICSI), where sperm are coated with exogenous DNA and injected into oocytes. The piezo impact drive, attached to the injecting micropipette holder, […]

Embryo Microinjection Station • Leica ASTP •

Station including Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system combines the operation of both microscope and micromanipulators into one user-oriented control unit (first motorized integrated transgenic platform). […]

Publications