The Mouse Genetics Engineering Center (Centre d’Ingénierie Génétique Murine, CIGM) is the Institut Pasteur’s transgenic mouse core facility. Since its creation in 2003, the CIGM aims to generate new models of transgenic mice by classical or targeted transgenesis. Classical (additive) transgenesis, which includes lentiviral transgenesis, is based on a random insertion of an exogenous gene. Targeted transgenesis consists in a deletion or a specific modification in an endogenous locus using homologous recombination in Embryonic Stem (ES) cells or, more recently, by directly using specific nucleases. Preferentially used by the Institut Pasteur scientific community, the services of CIGM are also available to outside customers interested in new transgenic, knock-down, knock-out (KO) or knock-in (KI) mouse/rat models. The core facility also provides expert advice regarding the design of gene constructs suitable for additive/targeted transgenesis and homologous recombination experiments.
Equipments
Embryo Microinjection Station • Nikon TE2000 •
Microscope +Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system (manual at the microscope level) allow, like the ASTP station above-mentioned, the storage and recall of positions and the […]
Embryo Reimplantation Station • Nikon Stereomicroscopes SMZ800 (episcopic base/diascopic base) •
The diascopic stereomicroscope allows embryo counting and the preparation of the reimplantation pipette. The stereomicroscope coupled to a coaxial Episcopic Illuminator (Fibber Optic Light Source) offers bright illumination over the entire surface of the […]
Eukaryotic Cell Electroporator • Bio-Rad Gene Pulser X Cell Total System •
The electroporator (with the appropriated parameters) gives the necessary pulse for the entering of the gene targeting vector or recombinase-expressing plasmid into the Embryonic Stem (ES) Cells.
XY Clone Laser System • Hamilton Thorne Bioscience •
The laser module, attached just like a typical objective, permits non-contact ablation of targeted membranes. It is mainly used in transgenesis for ablation of the zona pellucida for microinjection of eight-cell and morula stage […]
Piezo Impact Micromanipulator • PRIME TECH, Dipsi Industrie •
The piezo-assisted microinjection increases success rates for transgenesis by IntraCytoplasmic Sperm Injection (ICSI), where sperm are coated with exogenous DNA and injected into oocytes. The piezo impact drive, attached to the injecting micropipette holder, […]
Embryo Microinjection Station • Leica ASTP •
Station including Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system combines the operation of both microscope and micromanipulators into one user-oriented control unit (first motorized integrated transgenic platform). […]
Members
Former Members
Projects
Publications
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2018A Human Polymorphism in CHRNA5 Is Linked to Relapse to Nicotine Seeking in Transgenic Rats, Curr. Biol. 2018 Oct;28(20):3244-3253.e7.
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2017Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia, Oncotarget 2018 Jan;9(3):3779-3793.
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2017MITF – A controls branching morphogenesis and nephron endowment, PLoS Genet. 2017 12;13(12):e1007093.
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2016The circadian gene Arntl2 on distal mouse chromosome 6 controls thymocyte apoptosis, Mamm. Genome 2017 02;28(1-2):1-12.
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2016The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination, Oncotarget 2016 Aug;7(35):56083-56106.
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2016Generation of a Double KO Mouse by Simultaneous Targeting of the Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key Steps from Design to Genotyping, J Genet Genomics 2016 05;43(5):329-40.
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2016Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts, Nat Commun 2016 Jan;7:10324.
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2015Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer, Biol Open 2015 Nov;4(12):1614-24.
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2014Human hematopoietic reconstitution and HLA-restricted responses in nonpermissive alymphoid mice, J. Immunol. 2014 Aug;193(3):1504-11.
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2014A novel nodal enhancer dependent on pluripotency factors and smad2/3 signaling conditions a regulatory switch during epiblast maturation, PLoS Biol. 2014 Jun;12(6):e1001890.
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Contact
Phone: 01 44 38 95 66 Email: francina.langa-vives@pasteur.fr Centre d’Ingénierie Génétique Murine Bâtiment François Jacob 1er étage 28, rue du Dr. Roux 75015, Paris France