The Mouse Genetics Engineering Center (Centre d’Ingénierie Génétique Murine, CIGM) is the Institut Pasteur’s transgenic mouse core facility. Since its creation in 2003, the CIGM aims to generate new models of transgenic mice by classical or targeted transgenesis. Classical (additive) transgenesis, which includes lentiviral transgenesis, is based on a random insertion of an exogenous gene. Targeted transgenesis consists in a deletion or a specific modification in an endogenous locus using homologous recombination in Embryonic Stem (ES) cells or, more recently, by directly using specific nucleases. Preferentially used by the Institut Pasteur scientific community, the services of CIGM are also available to outside customers interested in new transgenic, knock-down, knock-out (KO) or knock-in (KI) mouse/rat models. The core facility also provides expert advice regarding the design of gene constructs suitable for additive/targeted transgenesis and homologous recombination experiments.
Members
Former Members
Projects
Equipments
Embryo Microinjection Station • Nikon TE2000 •
Microscope +Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system (manual at the microscope level) allow, like the ASTP station above-mentioned, the storage and recall of positions and the […]
Embryo Reimplantation Station • Nikon Stereomicroscopes SMZ800 (episcopic base/diascopic base) •
The diascopic stereomicroscope allows embryo counting and the preparation of the reimplantation pipette. The stereomicroscope coupled to a coaxial Episcopic Illuminator (Fibber Optic Light Source) offers bright illumination over the entire surface of the […]
Eukaryotic Cell Electroporator • Bio-Rad Gene Pulser X Cell Total System •
The electroporator (with the appropriated parameters) gives the necessary pulse for the entering of the gene targeting vector or recombinase-expressing plasmid into the Embryonic Stem (ES) Cells.
XY Clone Laser System • Hamilton Thorne Bioscience •
The laser module, attached just like a typical objective, permits non-contact ablation of targeted membranes. It is mainly used in transgenesis for ablation of the zona pellucida for microinjection of eight-cell and morula stage […]
Piezo Impact Micromanipulator • PRIME TECH, Dipsi Industrie •
The piezo-assisted microinjection increases success rates for transgenesis by IntraCytoplasmic Sperm Injection (ICSI), where sperm are coated with exogenous DNA and injected into oocytes. The piezo impact drive, attached to the injecting micropipette holder, […]
Embryo Microinjection Station • Leica ASTP •
Station including Transferman NK2 Micromanipulators and Femtojet, Cell Tram Air, Cell Tram Oil MIcroinjectors. The system combines the operation of both microscope and micromanipulators into one user-oriented control unit (first motorized integrated transgenic platform). […]
Publications
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2017MITF – A controls branching morphogenesis and nephron endowment, PLoS Genet. 2017 12;13(12):e1007093.
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2015Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer, Biol Open 2015 Nov;4(12):1614-24.
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2014Human hematopoietic reconstitution and HLA-restricted responses in nonpermissive alymphoid mice, J. Immunol. 2014 Aug;193(3):1504-11.
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2014Associations of HLA-A, HLA-B and HLA-C alleles frequency with prevalence of herpes simplex virus infections and diseases across global populations: implication for the development of an universal CD8+ T-cell epitope-based vaccine, Hum. Immunol. 2014 Aug;75(8):715-29.
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2014Protective role of LGP2 in influenza virus pathogenesis, J. Infect. Dis. 2014 Jul;210(2):214-23.
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2013HLA-A*01:03, HLA-A*24:02, HLA-B*08:01, HLA-B*27:05, HLA-B*35:01, HLA-B*44:02, and HLA-C*07:01 monochain transgenic/H-2 class I null mice: novel versatile preclinical models of human T cell responses, J. Immunol. 2013 Jul;191(2):583-93.
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2013Penicillin resistance compromises Nod1-dependent proinflammatory activity and virulence fitness of neisseria meningitidis, Cell Host Microbe 2013 Jun;13(6):735-45.
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2012Generation and characterization of a tamoxifen inducible Msx1(CreERT2) knock-in allele, Genesis 2013 Feb;51(2):110-9.
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2012The four and a half LIM-only protein 2 regulates liver homeostasis and contributes to carcinogenesis, J. Hepatol. 2012 Nov;57(5):1029-36.
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2012Loss of central and peripheral CD8+ T-cell tolerance to HFE in mouse models of human familial hemochromatosis, Eur. J. Immunol. 2012 Apr;42(4):851-62.
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Contact
Phone: 01 44 38 95 66
Email: francina.langa-vives@pasteur.fr
Centre d’Ingénierie Génétique Murine
Bâtiment François Jacob 1er étage
28, rue du Dr. Roux
75015, Paris
France