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  • whocc
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  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
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  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Service

Secondary and tertiary structures of macromolecules

Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
02
Apr 2015
Status
Ongoing
Members
1
Structures
1

About

Circular dichroism (CD) allows the measurement of secondary and tertiary structures, the analyse of conformational changes, the kinetic of folding and the thermal and chemical stability of folding.

aviv300

Fluorescence spectroscopy gives access to the analysis of tertiary conformations, of thermal and chemical stability, and of molecular interactions through the measure of fluorescence intensity, anisotropy and life-time.

pti

Infrared spectroscopy (FTIR) allows the measurement of secondary structures of macromolecules, in particular proteins with high ß sheet content.

ftirjasco

Access Protocol

PFBMI equipments are available for all scientists worldwide, but a priority is given to members of Institut Pasteur and the Institut Pasteur International Network.
Requests can be submitted at any time  (biophysique@pasteur.fr) attaching a project submission form.

Equipments

Circular Dichroism • AVIV • 215

The PFBMI has an Aviv 215 instrument with accessories for the measurement of total fluorescence and to perform titrations. Studying macromolecules (and in particular proteins) by circular dichroism allows to obtain information about their […]

Fluorescence Spectroscopy • Photon Technology International • Quantamaster QM4CW

Fluorescence spectroscopy is a versatile technique which allows to analyze, at steady-state or in real time, the variations of the environment of fluorescent probes, either intrinsic (for instance tryptophans in the case of proteins) […]

Infrared spectroscopy (FTIR) • Jasco • 6100

Fourier Transform Infra Red (FTIR) spectroscopy allows to analyze the secondary structure of macromolecules by measuring absorption frequencies of some specific chemical groups (notably, for proteins, that of the amide bond). Informations given by FTIR are […]