The immune system comprises a collection of specialized cells and organs that fight infections and can survey cancer cells. Immune responses to pathogens and tumors are highly coordinated events that take place in complex and specialized tissue microenvironments. An integrated view of innate and adaptive immune responses to infections and cancer requires a better understanding of how immune cells communicate and fulfill their task in vivo. In addition, it is essential to unravel how pathogens and tumor microenvironment subvert their functions. Recent progresses in intravital two-photon imaging offer a novel perspective to address some of these critical issues in physiological settings. By further developping functional in vivo imaging, we aim at identifying critical aspects of T cell and NK cell activation and function during tumor growth, during infection by an intracellular parasite and during transplantation. Elucidating the fundamental mechanisms underlying the regulation of innate and adaptive immune responses in vivo will delineate basic principles to develop or improve immunotherapeutical strategies to treat cancer and infectious diseases.
The immune system is made up of specialized cells and tissues that act together to fight microbial infections and survey the body for malignant transformation. Immune responses are highly orchestrated and dynamic events that […]
Lymphocyte activation in lymphoid organs
Adaptive immune responses are initiated in secondary lymphoid organs through cellular interactions between antigen-specific T cells and antigen-presenting dendritic cells. One of the aim of our lab is to better understand how T cells […]
Immune responses during infections
Intracellular pathogens have developed strategies to survive in infected cells and reduce their recognition by the innate and adaptive immune systems. Our lab wishes to define the mechanisms influencing the recognition of infected cells […]
Immune responses following transplantation
Transplant rejection involves a coordinated attack of donor cells by the innate and the adaptive immune system of the host. To gain insight into this dynamic process, we have developed an ear skin graft […]
Immune responses against tumors
Cytotoxic T cells and NK cells (CTLs) are key players of anti-tumor immune responses. Despite our fundamental knowledge on the interplay between the immune system and tumor microenvironments, we have a poor understanding on […]
Dynamic In Situ Cytometry
The Dynamic In Situ Cytometry (DISC) is a new approach that combines advantages of both intravital imaging and flow cytometry analysis. With the help of the custom designed DISCit software, the DISC provides the opportunity […]
2018CXCR3/CXCL10 Axis Shapes Tissue Distribution of Memory Phenotype CD8+ T Cells in Nonimmunized Mice, J. Immunol. 2018 Jan;200(1):139-146.
2017Microchannels for the Study of T Cell Immunological Synapses and Kinapses, Methods Mol. Biol. 2017;1584:347-354.
2017In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes, Methods Mol. Biol. 2017;1584:559-568.
2016The PD-1 Axis Enforces an Anatomical Segregation of CTL Activity that Creates Tumor Niches after Allogeneic Hematopoietic Stem Cell Transplantation, Immunity 2016 Jan;44(1):143-54.
2015In vivo imaging of inflammasome activation reveals a subcapsular macrophage burst response that mobilizes innate and adaptive immunity, Nat. Med. 2016 Jan;22(1):64-71.
2015Induction, Propagation, and Activity of Host Nitric Oxide: Lessons from Leishmania Infection, Trends Parasitol. 2015 Dec;31(12):653-64.
2015Signal strength regulates antigen-mediated T-cell deceleration by distinct mechanisms to promote local exploration or arrest, Proc. Natl. Acad. Sci. U.S.A. 2015 Sep;112(39):12151-6.
2014Semaphorin 3F and neuropilin-2 control the migration of human T-cell precursors, PLoS ONE 2014;9(7):e103405.
2014Collective nitric oxide production provides tissue-wide immunity during Leishmania infection, J. Clin. Invest. 2014 Apr;124(4):1711-22.
2013Visualizing how T cells collect activation signals in vivo, Curr. Opin. Immunol. 2014 Feb;26:56-62.
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