Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share
Starting Date
15
Jun 2015
Status
Ongoing
Members
5
Structures
1
Publications
23

About

Adaptive immune responses are initiated in secondary lymphoid organs through cellular interactions between antigen-specific T cells and antigen-presenting dendritic cells. One of the aim of our lab is to better understand how T cells collect activation signals in vivo. Using intravital multiphoton imaging, we are therefore studying how T cell-DC contacts are regulated and how activation signals are delivered during individual interactions. These studies help identify fundamental mechanisms shaping the efficacy of T cell activation and differentiation. Recently, we have extended these questions to natural killer cells.

activation movie 1

T cells forming stables interactions with dendritic cells in the lymph node upon antigen recognition. T cells (red) and dendritic cells (green) are imaged in the popliteal lymph node of a live mouse. Antigen is delivered to DCs at t=0.
Click here to see the movie

activation movie 2

Dynamics of the immunological synapse during T cell-B cell interactions in the lymph node. T cells expressed a LAT-GFP fusion protein. B cells are shown in red.
Click here to see the movie

activation movie 3T cell functional heterogeneity is initiated prior to the first cell division. T cells isolated from an IFN-g YFP reporter mice and labeled with ta red are imaged during their first division after activation. Yellow and green T cells have activated the IFN-g gene at the time of division, whereas red T cells have not.
Click here to see the movie

activation movie 4

In vivo imaging TCR signaling in real-time using CD62L shedding. GFP-expressing T cells (green) are labeled in vivo with fluorescent anti-CD62L Fab (red). Antigen is delivered at t=0 resulting in T cell stop and progressive decrease of CD62L staining. Intravital imaging was performed in the spleen.
Click here to see the movie

activation movie 5NK cell motility in lymph nodes. NK cells (red), T cells (blue) and DCs (green) are imaged at steady state in the lymph node.
Click here to see the movie

 

Fundings

References