Timeline

Daniel Ladant

Head of Structure

07 Feb 2024

news

Des moteurs moléculaires produisent l’énergie nécessaire pour nourrir les bactéries

20 Nov 2023

publication

B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles.

25 Sep 2023

publication

Functional and structural insights into the multi-step activation and catalytic mechanism of bacterial ExoY nucleotidyl cyclase toxins bound to actin-profilin

19 Jul 2023

publication

Animal Toxins: A Historical Outlook at the Institut Pasteur of Paris.

23 Jun 2023

publication

Immune responses and protection against Streptococcus pneumoniae elicited by recombinant Bordetella pertussis adenylate cyclase (CyaA) carrying fragments of pneumococcal surface protein A, PspA.

25 May 2023

event

The 29th Meeting on Toxinology is now being organized!

10 Feb 2023

publication

Editorial of the Special Issue “Toxins: Mr Hyde or Dr Jekyll?”.

07 Feb 2023

publication

Interaction network among de novo purine nucleotide biosynthesis enzymes in Escherichia coli.

08 Oct 2022

publication

A Robust and Sensitive Spectrophotometric Assay for the Enzymatic Activity of Bacterial Adenylate Cyclase Toxins.

08 Sep 2022

event

28th Meeting on Toxinology (RT28) – November 28th-29th, 2022

09 Aug 2022

publication

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium.

01 May 2021

publication

A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells.

22 Jan 2021

publication

Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Vaccine Development and Other Biotechnological Purposes.

23 Jul 2019

project

Detection and countermeasures against bacterial toxins

21 Jun 2019

publication

Post-translational acylation controls the folding and functions of the CyaA RTX toxin.

01 Mar 2019

publication

Editorial: Pathogens and disease special issue ETOX18

13 Feb 2019

team

UMR3528 – Structural Molecular Biology and Infectious Process

13 Feb 2019

publication

The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro.

13 Nov 2018

event

25th Meeting (RT25) of the French Society of Toxinology (SFET) : “Toxins and Health: from Molecules to Organisms”.

12 Nov 2018

publication

Differential regulation of actin-activated nucleotidyl cyclase virulence factors by filamentous and globular actin

30 Oct 2018

publication

The extreme C terminus of the effector ExoY is crucial for binding to its eukaryotic activator, F-actin

05 Sep 2018

event

Host cell chaperones during toxin translocation in cells

05 Sep 2018

event

Streptococcus pneumoniae: Development of protein-based vaccines and characterization of immune responses in animal models with high susceptibility to infection

20 Jul 2018

publication

Bioengineering of Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

01 May 2018

publication

Compartmentalized Cyclic AMP Production by the and Adenylate Cyclase Toxins Differentially Affects the Immune Synapse in T Lymphocytes

01 Jan 2018

publication

A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway Cloning

16 Dec 2017

publication

ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview

07 Dec 2017

publication

Lack of MHC class II molecules favors CD8 T-cell infiltration into tumors associated with an increased control of tumor growth

06 Apr 2017

event

ETOX18 – European Workshop on Bacterial Protein Toxins

03 Apr 2017

publication

Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase-Based Two-Hybrid (BACTH) Technique

10 Feb 2017

publication

Stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis

01 Jan 2017

publication

Protein-Protein Interaction: Bacterial Two-Hybrid

01 Jan 2017

publication

Defining Membrane Protein Topology Using pho-lac Reporter Fusions

05 Dec 2016

publication

Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins

20 Nov 2015

project

Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA

18 Nov 2015

project

Molecular crowding

16 Sep 2015

publication

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion.

15 Sep 2015

project

Clinical development of cancer vaccines

23 Aug 2015

publication

Structure and function of RTX toxins.

17 Aug 2015

project

CyaA secretion, folding and translocation across membrane

08 Jul 2015

project

Regulatory roles of signaling Nucleotides

08 Jul 2015

project

In vivo analysis of protein-protein interactions

08 Jul 2015

project

Recombinant CyaA toxin as an innovative vaccine vehicle

03 Jul 2015

project

Structure-function studies of the CyaA toxin

29 Jun 2015

publication

Rapamycin Impairs Antitumor CD8+ T-cell Responses and Vaccine-Induced Tumor Eradication

11 May 2015

team

Biochemistry of Macromolecular Interactions

11 Feb 2015

publication

Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis

31 Dec 2014

publication

Disorder-to-order transition in the CyaA toxin RTX domain: implications for toxin secretion

17 Sep 2014

publication

Calcium, acylation, and molecular confinement favor folding of Bordetella pertussis adenylate cyclase CyaA toxin into a monomeric and cytotoxic form

14 Jul 2014

publication

Antigen targeting to CD11b+ dendritic cells in association with TLR4/TRIF signaling promotes strong CD8+ T cell responses

24 Jun 2014

publication

ExoY from Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for cGMP and cUMP formation

06 Jun 2014

publication

Analysis of MreB interactors in Chlamydia reveals a RodZ homolog but fails to detect an interaction with MraY

25 Nov 2013

publication

A Gateway(®) -compatible bacterial adenylate cyclase-based two-hybrid system

24 Sep 2013

publication

Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin

31 Jul 2013

publication

Molecular crowding stabilizes both the intrinsically disordered calcium-free state and the folded calcium-bound state of a repeat in toxin (RTX) protein

10 Aug 2012

publication

The β-lactam resistance protein Blr, a small membrane polypeptide, is a component of the Escherichia coli cell division machinery

07 Jun 2012

publication

Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division

15 Apr 2012

publication

3′-5′ phosphoadenosine phosphate is an inhibitor of PARP-1 and a potential mediator of the lithium-dependent inhibition of PARP-1 in vivo

12 Jan 2012

publication

Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin

01 Jan 2012

publication

Mean net charge of intrinsically disordered proteins: experimental determination of protein valence by electrophoretic mobility measurements

01 Jan 2012

publication

Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods

22 Jul 2011

publication

Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB

15 Mar 2011

publication

Calcium-induced folding of intrinsically disordered repeat-in-toxin (RTX) motifs via changes of protein charges and oligomerization states

01 Dec 2010

publication

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin

20 Aug 2010

publication

Induction of anti-Tat neutralizing antibodies by the CyaA vector targeting dendritic cells: influence of the insertion site and of the delivery of multicopies of the dominant Tat B-cell epitope

07 Jun 2010

publication

Use of allostery to identify inhibitors of calmodulin-induced activation of Bacillus anthracis edema factor

22 Jan 2010

publication

Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin

19 Jan 2010

publication

Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin

17 Nov 2008

publication

RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion

31 Oct 2008

publication

Characterization of YmgF, a 72-residue inner membrane protein that associates with the Escherichia coli cell division machinery

15 Sep 2007

publication

Eradication of large tumors in mice by a tritherapy targeting the innate, adaptive, and regulatory components of the immune system

20 Apr 2006

publication

Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin

01 Apr 2006

publication

An increase in antimycobacterial Th1-cell responses by prime-boost protocols of immunization does not enhance protection against tuberculosis

21 Feb 2006

publication

Delivery of the HIV-1 Tat protein to dendritic cells by the CyaA vector induces specific Th1 responses and high affinity neutralizing antibodies in non human primates

01 Aug 2005

publication

Induction of neutralizing antibodies and Th1-polarized and CD4-independent CD8+ T-cell responses following delivery of human immunodeficiency virus type 1 Tat protein by recombinant adenylate cyclase of Bordetella pertussis

01 Apr 2005

publication

Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis

15 Jan 2005

publication

Eradication of established tumors by vaccination with recombinant Bordetella pertussis adenylate cyclase carrying the human papillomavirus 16 E7 oncoprotein

16 Dec 2004

publication

Bordetella pertussis adenylate cyclase delivers chemically coupled CD8+ T-cell epitopes to dendritic cells and elicits CTL in vivo

01 Mar 2004

publication

Relief of catabolite repression in a cAMP-independent catabolite gene activator mutant of Escherichia coli

01 Dec 2003

publication

Recombinant adenylate cyclase toxin of Bordetella pertussis induces cytotoxic T lymphocyte responses against HLA*0201-restricted melanoma epitopes

01 Sep 2003

publication

In vitro selection for enzymatic activity: a model study using adenylate cyclase

01 Aug 2003

publication

Human immunodeficiency virus (HIV) type 1 transframe protein can restore activity to a dimerization-deficient HIV protease variant

28 Jul 2003

publication

Interaction of Bordetella pertussis adenylate cyclase with CD11b/CD18: Role of toxin acylation and identification of the main integrin interaction domain

01 May 2003

publication

Efficient display of two enzymes on filamentous phage using an improved signal sequence

01 Nov 2002

publication

In vivo receptor-mediated delivery of a recombinant invasive bacterial toxoid to CD11c + CD8 alpha -CD11bhigh dendritic cells.

01 Jun 2002

publication

Two-hybrid systems and their usage in infection biology

01 Jan 2000

publication

Bordetella pertussis adenylate cyclase toxin: a vehicle to deliver CD8-positive T-cell epitopes into antigen-presenting cells.

15 Feb 1999

publication

A little guide for advanced search:

Des moteurs moléculaires produisent l’énergie nécessaire pour nourrir les bactéries

B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles.

Functional and structural insights into the multi-step activation and catalytic mechanism of bacterial ExoY nucleotidyl cyclase toxins bound to actin-profilin

Animal Toxins: A Historical Outlook at the Institut Pasteur of Paris.

Immune responses and protection against Streptococcus pneumoniae elicited by recombinant Bordetella pertussis adenylate cyclase (CyaA) carrying fragments of pneumococcal surface protein A, PspA.

The 29th Meeting on Toxinology is now being organized!

Editorial of the Special Issue “Toxins: Mr Hyde or Dr Jekyll?”.

Interaction network among de novo purine nucleotide biosynthesis enzymes in Escherichia coli.

A Robust and Sensitive Spectrophotometric Assay for the Enzymatic Activity of Bacterial Adenylate Cyclase Toxins.

28th Meeting on Toxinology (RT28) – November 28th-29th, 2022

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium.

A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells.

Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Vaccine Development and Other Biotechnological Purposes.

Detection and countermeasures against bacterial toxins

Post-translational acylation controls the folding and functions of the CyaA RTX toxin.

Editorial: Pathogens and disease special issue ETOX18

UMR3528 – Structural Molecular Biology and Infectious Process

The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro.

25th Meeting (RT25) of the French Society of Toxinology (SFET) : “Toxins and Health: from Molecules to Organisms”.

Differential regulation of actin-activated nucleotidyl cyclase virulence factors by filamentous and globular actin

The extreme C terminus of the effector ExoY is crucial for binding to its eukaryotic activator, F-actin

Host cell chaperones during toxin translocation in cells

Streptococcus pneumoniae: Development of protein-based vaccines and characterization of immune responses in animal models with high susceptibility to infection

Bioengineering of Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

Compartmentalized Cyclic AMP Production by the and Adenylate Cyclase Toxins Differentially Affects the Immune Synapse in T Lymphocytes

A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway Cloning

ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview

Lack of MHC class II molecules favors CD8 T-cell infiltration into tumors associated with an increased control of tumor growth

ETOX18 – European Workshop on Bacterial Protein Toxins

Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase-Based Two-Hybrid (BACTH) Technique

Stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis

Protein-Protein Interaction: Bacterial Two-Hybrid

Defining Membrane Protein Topology Using pho-lac Reporter Fusions

Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins

Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA

Molecular crowding

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion.

Clinical development of cancer vaccines

Structure and function of RTX toxins.

CyaA secretion, folding and translocation across membrane

Regulatory roles of signaling Nucleotides

In vivo analysis of protein-protein interactions

Recombinant CyaA toxin as an innovative vaccine vehicle

Structure-function studies of the CyaA toxin

Rapamycin Impairs Antitumor CD8+ T-cell Responses and Vaccine-Induced Tumor Eradication

Biochemistry of Macromolecular Interactions

Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis

Disorder-to-order transition in the CyaA toxin RTX domain: implications for toxin secretion

Calcium, acylation, and molecular confinement favor folding of Bordetella pertussis adenylate cyclase CyaA toxin into a monomeric and cytotoxic form

Antigen targeting to CD11b+ dendritic cells in association with TLR4/TRIF signaling promotes strong CD8+ T cell responses

ExoY from Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for cGMP and cUMP formation

Analysis of MreB interactors in Chlamydia reveals a RodZ homolog but fails to detect an interaction with MraY

A Gateway(®) -compatible bacterial adenylate cyclase-based two-hybrid system

Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin

Molecular crowding stabilizes both the intrinsically disordered calcium-free state and the folded calcium-bound state of a repeat in toxin (RTX) protein

The β-lactam resistance protein Blr, a small membrane polypeptide, is a component of the Escherichia coli cell division machinery

Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division

3′-5′ phosphoadenosine phosphate is an inhibitor of PARP-1 and a potential mediator of the lithium-dependent inhibition of PARP-1 in vivo

Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin

Mean net charge of intrinsically disordered proteins: experimental determination of protein valence by electrophoretic mobility measurements

Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods

Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB

Calcium-induced folding of intrinsically disordered repeat-in-toxin (RTX) motifs via changes of protein charges and oligomerization states

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin

Induction of anti-Tat neutralizing antibodies by the CyaA vector targeting dendritic cells: influence of the insertion site and of the delivery of multicopies of the dominant Tat B-cell epitope

Use of allostery to identify inhibitors of calmodulin-induced activation of Bacillus anthracis edema factor

Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin

Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin

RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion

Characterization of YmgF, a 72-residue inner membrane protein that associates with the Escherichia coli cell division machinery

Eradication of large tumors in mice by a tritherapy targeting the innate, adaptive, and regulatory components of the immune system

Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin

An increase in antimycobacterial Th1-cell responses by prime-boost protocols of immunization does not enhance protection against tuberculosis

Delivery of the HIV-1 Tat protein to dendritic cells by the CyaA vector induces specific Th1 responses and high affinity neutralizing antibodies in non human primates

Induction of neutralizing antibodies and Th1-polarized and CD4-independent CD8+ T-cell responses following delivery of human immunodeficiency virus type 1 Tat protein by recombinant adenylate cyclase of Bordetella pertussis

Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis

Eradication of established tumors by vaccination with recombinant Bordetella pertussis adenylate cyclase carrying the human papillomavirus 16 E7 oncoprotein

Bordetella pertussis adenylate cyclase delivers chemically coupled CD8+ T-cell epitopes to dendritic cells and elicits CTL in vivo

Relief of catabolite repression in a cAMP-independent catabolite gene activator mutant of Escherichia coli