On the cover: Type IV pili (T4P), long and flexible appendages at the surface of many bacteria, are critical virulence factors. Pili of enterohemorrhagic E. coli (EHEC), an important human pathogen, are polymers of the major subunit PpdD. In the quest for drug and vaccine discovery against such pathogens, unraveling the detailed organization and dynamics of PpdD pili will be instrumental. By combining NMR and cryo-EM, Bardiaux et al. (pp. 1082–1093) add a new page to the coloring book of T4P atomic models. The EHEC T4P structure revealed a large extended region of the pilin stem correlating with extremely high pilus flexibility, along with residues crucial for its assembly and stability. Image created by Benjamin Bardiaux (CNRS/Institut Pasteur, Paris).
Protein secretion in bacteria is highly specific. Substrates secreted via the Type II secretion nanomachines are fully folded prior to secretion and are specifically recognized in their native state. In some cases, they even […]
ViBrANT (Viral and Bacterial Adhesin Network Training)
The Innovative Training Network ViBrANT (Viral and Bacterial Adhesin Network Training) brings together world-leading European scientists to train 15 graduate students (early stage researchers) in interdisciplinary research into viral and bacterial adhesins. ViBrANT is […]
ARIA for hybrid structure determination on the cloud
Protein structure determination is crucial for understanding protein function, as it paves the way to the discovery of new drugs and of new approaches to control pathological biological processes. The recent advances in structural […]
A Bayesian Framework for Cellular Structural Biology Objective : “The functioning of a single cell or organism is governed by the laws of chemistry and physics. The bridge from biology to chemistry and physics […]
About Bip:Bip The project Bip:Bip promotes an integrated use of known and modelled three-dimensional structures of proteins as an aid in complete genome annotation. The architectural organization of biological macromolecules is essential for the […]
Bacterial Transmembrane Signaling
Bacteria display several mechanisms to adapt to their host-environment and successfully colonise it. One important mechanism of adaptation concerns the regulation of gene expression in response to external signals such as the presence of […]
ARIA (Ambiguous Restraints for Iterative Assignment)
Our computer program ARIA (Ambiguous Restraints for Iterative Assignment) is a software for automated NOE assignment and NMR structure calculation. It speeds up and automatizes the assignment process through the use of an iterative […]
ARIAweb: a server for automated NMR structure calculation
The ARIAweb server provides access to the main ARIA software functionalities (NMR data conversion and structure calculation). In addition, structure calculation results can be easily visualised, with a dedicated molecular viewer and graphical displays […]
Since Dec 2012, permanent research associate at CNRS UMR3528 and Unité de Bioinformatique Structurale.
2021Structural and molecular basis of cross-seeding barriers in amyloids., Proc Natl Acad Sci U S A 2021 Jan; 118(1): .
2020Aβ(1-42) tetramer and octamer structures reveal edge conductivity pores as a mechanism for membrane damage., Nat Commun 2020 Jun; 11(1): 3014.
2020ARIAweb: a server for automated NMR structure calculation., Nucleic Acids Res. 2020 May; (): .
2019Structure and function of minor pilins of type IV pili, Med. Microbiol. Immunol. 2019 Nov;.
2019Quantitative Structural Interpretation of Protein Crosslinks, Structure 2019 Nov;.
2019Dynamics of a type 2 secretion system pseudopilus unraveled by complementary approaches, J. Biomol. NMR 2019 Jul;73(6-7):293-303.
2019Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus., Structure. 2019 Apr 9. doi: 10.1016/j.str.2019.03.021..
2019Single-site phosphorylation within the His-tag sequence attached to a recombinant protein, Anal. Biochem. 2019 Feb;.
2018Biogenesis and structure of a type VI secretion baseplate, Nat Microbiol 2018 Oct;.
2018Structure and dynamics of Helicobacter pylori nickel-chaperone HypA: an integrated approach using NMR spectroscopy, functional assays and computational tools, J. Biol. Inorg. Chem. 2018 Sep;.
+View full list of publications