On the cover: Type IV pili (T4P), long and flexible appendages at the surface of many bacteria, are critical virulence factors. Pili of enterohemorrhagic E. coli (EHEC), an important human pathogen, are polymers of the major subunit PpdD. In the quest for drug and vaccine discovery against such pathogens, unraveling the detailed organization and dynamics of PpdD pili will be instrumental. By combining NMR and cryo-EM, Bardiaux et al. (pp. 1082–1093) add a new page to the coloring book of T4P atomic models. The EHEC T4P structure revealed a large extended region of the pilin stem correlating with extremely high pilus flexibility, along with residues crucial for its assembly and stability. Image created by Benjamin Bardiaux (CNRS/Institut Pasteur, Paris).
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Since Dec 2012, permanent research associate at CNRS UMR3528 and Unité de Bioinformatique Structurale.
Publications
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2019Dynamics of a type 2 secretion system pseudopilus unraveled by complementary approaches, J. Biomol. NMR 2019 May;.
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2019Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus., Structure. 2019 Apr 9. doi: 10.1016/j.str.2019.03.021..
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2019Single-site phosphorylation within the His-tag sequence attached to a recombinant protein, Anal. Biochem. 2019 Feb;.
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2018Biogenesis and structure of a type VI secretion baseplate, Nat Microbiol 2018 Oct;.
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2018Structure and dynamics of Helicobacter pylori nickel-chaperone HypA: an integrated approach using NMR spectroscopy, functional assays and computational tools, J. Biol. Inorg. Chem. 2018 Sep;.
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2018The protofilament architecture of a de novo designed coiled coil-based amyloidogenic peptide, J. Struct. Biol. 2018 May;.
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2018Minimal NMR distance information for rigidity of protein graphs, Discrete Appl Math 2019 Mar;256:91-104.
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20183D Structure Determination of Amyloid Fibrils using Solid-State NMR Spectroscopy, Methods 2018 Apr;.
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2018Ordering Protein Contact Matrices, Comput Struct Biotechnol J. 2018 Mar;16:140-56.
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2018Tuning interval Branch-and-Prune for protein structure determination, J Glob Optim. 2018 Sep; 72(1):109–27.
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