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© William Beaucardet
Reportage Laboratoire des Anticorps en Thérapie et Pathologie

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Désolé, cet article est seulement disponible en EN.

Services

Booking site
Single Cell Biomakers proposes various services to the community :
  •  Training for autonomous use of the equipment
  • Assisted use for a selection of technologies
  • Advice and technical support
  • Development of new of applications and pipelines
  • Scientific collaborations
  • Access to different data analysis software and project-tailored bioinformatics support for analysis of large-scale data generated by our technologies

Contact : biomarkers_team@pasteur.fr for more information.

Training @ Teaching

TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]

Équipements

Booking site

Single Cell Biomakers is an integrated technology platform for multidimensional cell phenotyping & molecular profiling. We propose single-cell cutting-edge technologies and data analysis pipelines. All technologies are bookable through the PPMS online equipment and training reservation site. The equipment is installed in six dedicated laboratories, with a significant part of the technologies situated within BSL2+ (biosafety level 2 +) environment allowing processing of human and infectious material. View a non-exhaustive list of pathogens currently manipulated in the laboratory.

Laboratories and equipment location

Cell phenotyping and sorting (Flow Cytometry Platform)

  •  – 3 Conventional cytometers (Symphony A5, LSRFortessa, Cytoflex for simultaneous detection of up to 28 parameters
  •  – Spectral cytometer (ID7000) for indept phenotyping (>40 parameters) and management of autofluorescence
  •  – Imaging cytometer MARKII (Luminex): spatial quantification of intra- and inter-cellular processes in cells in suspension
  •  – 7 Cell sorters (2 Astrios, 4 FACSAria Fusion, 1 FACSAria S6): simultaneous sorting of ≤ 6 cell populations, using ≤28 fluorophores in a single panel. 2 sorters  available in Bsl2+ environment for sorting of human and/or infectious material.
  •  – 2 Magnetic-based cell sorters (AutoMACS): pre-enrichment of rare cell populations.
  •  – MultiMACS: isolation of targeted cell populations from whole blood.

Cell culture and functional assays in Bsl2+ environment (Biomarkers Platform)

  • Fully-equipped cell culture facility
  • Hypoxia cabinet XVivo (Biospherix): manipulation and cell culture under controlled O2, CO2, N2, temperature and humidity
  • Seahorse (Agilent): measuring cellular glycolysis and mitochondrial respiration under controlled O2, CO2, N2, temperature and humidity
  • Incucyte S6 (Sartorius): short- and long-term live-content imaging in any conventional cell culture support
  • Amaxa nucleofector (Lonza): nucleofection

Multiplex and ultrasensitive protein quantification (immunoassays) (Biomarkers Platform)

  • Bioplex 200 (Biorad) coupled to DropArray (Curiox): quantification of ≤ 200 analytes in cell lysis, supernatants or body-fluids with pM sensitivity from reduced sample (5uL) and reagent volumes.
  • Simoa HD-x (Quanterix): digital ELISA for quantification of proteins with fM sensitivity
  • SP-X (Quanterix): ultrasensitive multiplex protein detection (10-plex)
  • Isolight  (Isoplexis): single -cell sectretome analysis (32-plex)

RNA/DNA profiling in bulk (Single Cell Platform)

  • Nanostring (Nanostring): direct multiplex RNA quantification (800-plex)
  • Bioanalyzer (Agilent): quality control of DNA, RNA, & proteins
  • Qubit: RNA-quantification

Multi-modal single cell profiling (Single Cell Platform)

  • 10xGenomics (3 instruments): single-cell transcriptomics, single-nuclei transcriptomics CITE-Seq, ATAC-Seq, TCR/BCR rearrangement. Available in Bsl2+ confinement for analysis of human and/or infectious cells.
    • Spatial transcriptomics (10xGenomics)
    • MARS-Seq
    • Biomark (Fluidigm): multiplex single cell real-time PCR (96-plex)

Data analysis

  • Schnapps –shiny application for analysis of scRNA-Seq data by biologists
  • FLowJo (BD) – a tool of choice for analysis of multidimensional flow cytometry data
  • Qlucore- user-friendly PCA tool for analysis of high-dimensional data

SP-X • •

Ultra-sensitive Simoa planar array technology. Allows multiplexing up to 10-plex. Benchtop form factor semi-automated offline assay prep.

HD-X Analyzer • •

Ultra-sensitive Simoa bead assay technology. Allows the multiplexing up to 6-plex. Floor-standing integrated system. Completely automated assay prep and detection.

IsoLight • •

The IsoLight is a hub for comprehensive proteomic profiling of each cell type across a large assay menu of single-cell and population chip and software products. With one instrument and companion analysis software, we […]

Meso QuickPlex SQ120 (MSD) • •

MSD technology combines electrochemiluminescence and MULTI‑ARRAY to provide unsurpassed sensitivity and multiplex functionality making it an exceptional detection system. MULTI-ARRAY technology is applied to MULTI-SPOT plates to enable precise quantitation of multiple analytes in […]

Intelliflex • •

The Luminex xMAP INTELLIFLEX is an advanced multiplexing platform that delivers accurate and reliable protein measurement results. It allows the multiplexing up to 500 analytes in a single sample volume. Designed for a 96- […]

Seahorse – Cell metabolism • •

Seahorse & Seahorse Mini Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well or 8-well miniplates . With four programmable ports for the addition of liquid media during active assay measurements, it is ideal […]

Incucyte – Live cell imaging • •

Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte SX5 supports HD phase-contrast, and 2 fluorescence modules (Green/Red, Green/Orange/NIR) and the FRET based Metabolism Optical Module at 4x, 10x […]

BioPlex 200 – protein quantification • •

BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]

Amaxa – nucleofector • •

Nucleofection in 96 well shuttle or in cuvette

2 • nCounter NanoString – Gene expression profiling • •

nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]

BioMark – gene expression profiling • •

BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]

2100 Bioanalyzer – RNA and DNA quality/quantity • •

Bioanalyzer  Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.

Qubit 2.0 – DNA/RNA quantification • •

Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.

ImageStreamX – Imaging cytometry • Cytek Biosciences • MarkII

ImageStreamX Mk II The Cytek® Amnis® ImageStream®X Mk II imaging flow cytometer combines the high throughput performance of flow cytometry with the imagery and functional insights of microscopy. This unique combination enables a broad […]

Membres

Cours

Pasteur Course Advanced immunology

This intensive six-week course, including practical work, lectures, and scientific analysis workshops, covers the fundamental themes of cellular and molecular immunology. For more information and program, please, visit the course webpage.

2025-11-17 09:00:00 2026-01-09 18:00:00 Europe/Paris Pasteur Course Advanced immunology This intensive six-week course, including practical work, lectures, and scientific analysis workshops, covers the fundamental themes of cellular and molecular immunology. For more information and program, please, visit the course webpage. Institut Pasteur, Rue du Docteur Roux, Paris, France

Pasteur Course Single cell gene expression and beyond

The course offers comprehensive theoretical and practical training in all stages of high-throughput single-cell profiling, from sample and library preparation to data analysis. Participants will learn to apply cutting-edge technologies for single-cell multi-omics and […]

2025-06-25 09:00:00 2025-07-02 18:00:00 Europe/Paris Pasteur Course Single cell gene expression and beyond The course offers comprehensive theoretical and practical training in all stages of high-throughput single-cell profiling, from sample and library preparation to data analysis. Participants will learn to apply cutting-edge technologies for single-cell multi-omics and […] Institut Pasteur, Rue du Docteur Roux, Paris, France

Single cell gene expression and beyond

Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […]

2022-06-30 09:00:00 2022-07-06 18:00:00 Europe/Paris Single cell gene expression and beyond Course Overview The course aims at providing theoretical and hands-on training for all steps of high-throughput single cell profiling, from single-cell sample and library preparation to data analysis, using state-of-the-art technological solutions. Program This […] 28 Rue du Docteur Roux, Paris, France

10th HKU-Pasteur Immunology Course: December 2-15, 2018

  ‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […]

2018-12-02 09:00:00 2018-12-15 18:00:00 Europe/Paris 10th HKU-Pasteur Immunology Course: December 2-15, 2018   ‘Quantitative Immunology’ will be the theme of the 10th anniversary of the HKU-Pasteur Immunology Course. We will explore the ongoing transition from reductionist studies, based on the application of genetic approaches in animal models, […] HKU-Pasteur Research Centre, Hong Kong

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Contact

Phone: 01.40.61.30.27
Email: biomarkers_team@pasteur.fr
Address : 25-28 Rue du Docteur Roux
75015 Paris France